Anti-inflammatory effect of different PRGF formulations on cutaneous surface

Anitua, Eduardo; Pino, Ander; Aspe, Libe; Martínez, MaIsabel; García, A.; Goñi, Felipe; Troya, María

doi:10.1016/j.jtv.2021.02.011

Cited by 12 publications

(6 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also provide the first evidence regarding the potential of PRGF to promote macrophage transition in peripheral nerves by reducing the M1-secreted inflammatory cytokines and number of M1 macrophages (CD68-positive cells) as well as an increase in the expression of M2 macrophage (CD206). Our results agree with other PRGF applications that inhibit the TNFα and IL-1β production by using in vitro rheumatoid arthritis (RA) model [ 29 , 54 ]. The nerve conduit created a relatively close environment after suture and bridged the two ends of an injured nerve.…”

Section: Discussionsupporting

confidence: 90%

“…Therefore, researchers have developed a PRP derivative known as platelet-rich growth factor (PRGF), through single slow centrifugation of whole blood which prevents the formation of a buffy coat that contains WBCs along with platelets [ 28 ]. PRGF is beneficial in reducing the production of inflammatory cytokines, such as TNFα and IL-1β for the management of atopic dermatitis condition [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Autologous Platelet-Rich Growth Factor Reduces M1 Macrophages and Modulates Inflammatory Microenvironments to Promote Sciatic Nerve Regeneration

et al. 2022

View full text Add to dashboard Cite

The failure of peripheral nerve regeneration is often associated with the inability to generate a permissive molecular and cellular microenvironment for nerve repair. Autologous therapies, such as platelet-rich plasma (PRP) or its derivative platelet-rich growth factors (PRGF), may improve peripheral nerve regeneration via unknown mechanistic roles and actions in macrophage polarization. In the current study, we hypothesize that excessive and prolonged inflammation might result in the failure of pro-inflammatory M1 macrophage transit to anti-inflammatory M2 macrophages in large nerve defects. PRGF was used in vitro at the time the unpolarized macrophages (M0) macrophages were induced to M1 macrophages to observe if PRGF altered the secretion of cytokines and resulted in a phenotypic change. PRGF was also employed in the nerve conduit of a rat sciatic nerve transection model to identify alterations in macrophages that might influence excessive inflammation and nerve regeneration. PRGF administration reduced the mRNA expression of tumor necrosis factor-α (TNFα), interleukin-1β (IL-1β), and IL-6 in M0 macrophages. Increased CD206 substantiated the shift of pro-inflammatory cytokines to the M2 regenerative macrophage. Administration of PRGF in the nerve conduit after rat sciatic nerve transection promoted nerve regeneration by improving nerve gross morphology and its targeted gastrocnemius muscle mass. The regenerative markers were increased for regrown axons (protein gene product, PGP9.5), Schwann cells (S100β), and myelin basic protein (MBP) after 6 weeks of injury. The decreased expression of TNFα, IL-1β, IL-6, and CD68+ M1 macrophages indicated that the inflammatory microenvironments were reduced in the PRGF-treated nerve tissue. The increase in RECA-positive cells suggested the PRGF also promoted angiogenesis during nerve regeneration. Taken together, these results indicate the potential role and clinical implication of autologous PRGF in regulating inflammatory microenvironments via macrophage polarization after nerve transection.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Autologous Platelet-Rich Growth Factor Reduces M1 Macrophages and Modulates Inflammatory Microenvironments to Promote Sciatic Nerve Regeneration

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In this sense, PRGF have previously shown to modulate inflammation by the attenuation of nuclear factor-κB pathway and the reduction of pro-inflammatory cytokines such as IL-1β, IL-6, IL-8 and TNF-α. 22 GF within PRGF may also terminate inflammation by restoring local cells to a non-inflammatory phenotype. This effect could be mediated by VEGF and HGF, which additionally protect the function of the endothelial barrier and promote angiogenesis increasing nutrient supply to the hair bulb.…”

Section: Discussionmentioning

confidence: 99%

“…20 Proteins within PRGF are also involved in antiinflammatory and anti-fibrotic responses that may exert an adjunctive effect when applied in combination with conventional drugs against FFA. [21][22][23] Hence, the aim of this study was to retrospectively analyze the adjuvant use of PRGF compared to the conventional treatment in the management of FFA.…”

Section: Introductionmentioning

confidence: 99%

Plasma Rich in Growth Factors as an Adjuvant Treatment for the Management of Frontal Fibrosing Alopecia: A Retrospective Observational Clinical Study

García,

Navarro,

Ramirez

et al. 2023

J Cutan Med Surg

Self Cite

View full text Add to dashboard Cite

Background Frontal fibrosing alopecia (FFA) is a scarring alopecia in which the exact etiopathogenesis has not been completely elucidated and the available treatments are not very effective. Plasma rich in growth factors (PRGF) has shown to induce folliculogenesis in hair loss related disorders. However, the scientific evidence when facing FFA is scarce. Objectives The aim of this study was to retrospectively analyze the adjuvant use of PRGF compared to the conventional treatment in the management of FFA. Methods Participants with clinically diagnosed FFA who had been treated with either conventional therapy (Control Group) or conventional therapy combined with PRGF (PRGF Group) were identified from the center’s medical records. The clinical assessment was based on the “Frontal Fibrosing Alopecia Severity Score” (FFASS), which was fulfilled during a period of two and 4 years. Results This study included 118 patients with clinically diagnosed FFA (Control Group: 57 and PRGF Group: 61). No adverse effects related to the treatments were observed. Both treatments showed to halt the steady progression of hair loss compared to baseline. PRGF treatment also induced significant hair regrowth compared to the Control Group. The scalp inflammation was reduced in response to treatments. The FFASS score indicated that PRGF Group improved the symptoms and severity of FFA in a significant manner. Conclusions The adjuvant use of PRGF may exert long-term beneficial effects on hair loss reduction and might reduce the symptoms and severity of FFA.

show abstract

“…The implantation process is a consequence of an inflammatory and anti-inflammatory equilibrium and an imbalance could lead to cases of RIF [ 25 ]. PRP has demonstrated anti-inflammatory action in several medical fields, namely, PRGF-Endoret [ 26 ]. The positive effects of PRP on implantation and gestation should occur via its anti-inflammatory molecules in the case of undetected or silent endometritis and even working over a possible underlying infection, thanks to the anti-microbial role of PRPs [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Potential of Plasma Rich in Growth Factors (PRGF-Endoret) to Enhance the Efficacy of Assisted Reproductive Techniques in Refractory Cases

Agirregoikoa¹,

Pablo²,

Fuente³

et al. 2022

Cureus

Self Cite

View full text Add to dashboard Cite

Aim: Nowadays, infertility problems affect a high percentage of couples. This study aimed to evaluate the effect of plasma rich in growth factors (PRGF-Endoret, hereafter PRGF) as a promising coadjuvant therapy in assisted reproductive techniques and its possible role in implantation and pregnancy rates. This retrospective study included 36 PRGF cycles in 27 women with one of the following reproductive disorders: recurrent implantation failure (n = 16), repeated abortion (n = 8), and thin endometrium (n = 3).Methods: PRGF was obtained from each patient and administered as three consecutive intrauterine instillations. The endometrial thickness was measured after each PRGF infusion and a good-quality embryo transfer was performed for every patient. Endometrial thickness, biochemical pregnancy, and miscarriage rate were the primary measured outcomes.Results: PRGF increased the endometrial growth respecting the initial thickness in all cases. The biochemical pregnancy rate determined as positive beta-human chorionic gonadotropin (β-hCG) was 59%, considering the total number of patients; the ongoing pregnancy percentage was 48%. PRGF application day was relevant with a significant probability of achieving pregnancy (p < 0.01) when the first PRGF infusion was carried out beyond 6.5 days after the first day of the woman's cycle and the second one beyond 9.5 days of the menstrual cycle.Conclusions: Intrauterine autologous PRGF infusion is a safe, easily accessible, and inexpensive therapy that could collaborate in fertility treatments by optimizing the endometrium for implantation and thus favoring the crosstalk between the embryo and the uterus improving the embryo-maternal dialogue.

show abstract

Anti-inflammatory effect of different PRGF formulations on cutaneous surface

Cited by 12 publications

References 45 publications

Autologous Platelet-Rich Growth Factor Reduces M1 Macrophages and Modulates Inflammatory Microenvironments to Promote Sciatic Nerve Regeneration

Autologous Platelet-Rich Growth Factor Reduces M1 Macrophages and Modulates Inflammatory Microenvironments to Promote Sciatic Nerve Regeneration

Plasma Rich in Growth Factors as an Adjuvant Treatment for the Management of Frontal Fibrosing Alopecia: A Retrospective Observational Clinical Study

Potential of Plasma Rich in Growth Factors (PRGF-Endoret) to Enhance the Efficacy of Assisted Reproductive Techniques in Refractory Cases

Contact Info

Product

Resources

About