Anti-inflammatory Effect of Ethanol Extract from Sargassum fulvellum on Lipopolysaccharide Induced Inflammatory Responses in RAW 264.7 Cells and Mice Ears

Jeong

et al. 2023

KSBB

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In this study, the anti-inflammatory effects of Monostroma crassidermum Tokida ethanol extract (MCEE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and croton oil-stimulated ICR mouse models were investigated. MCEE reduced the secretion of pro-inflammatory cytokines, including interleukin-6, IL-1β, tumor necrosis factor-α, and nitric oxide, which are non-toxic to RAW 264.7 cells. In vivo testing showed that the results in the MCEE-treated group (10-250 mg/kg. body weight) were similar to those in the prednisolone-treated group (10 mg/kg. body weight). Photomicrographs showed that the skin layer was thinned, and mast cell infiltration was reduced in the mouse-ear tissue. Therefore, these results suggested that MCEE exerts an anti-inflammatory effect, indicating the possible applications of MCEE as a natural anti-inflammatory material.

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Section: Inhibitory Effect Of No Productionsupporting

confidence: 88%

Anti-Inflammatory Effects of Monostroma crassidermum Tokida Ethanol Extract on Lipopolysaccharide-Stimulated RAW 264.7 Cells and Mice Ear Edema

Jeong

et al. 2023

KSBB

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“…Furthermore, binding of TNF-α and TNF-α receptors in the membrane of a macrophage causes the activation of NF-κB and other factors down stream during the inflammatory response [20]. In addition, IL-1β is known as a cytokine induced by caspase-1 in activated macrophages, and is critical for the response to infection as it influences the production of NO [21]. For this reason, the development of therapies targeting these pro-inflammatory cytokines is an important therapeutic strategy for treating a variety of inflammatory diseases.…”

Section: A -Fmentioning

confidence: 99%

Anti-Inflammatory Effects of Grasshopper Ketone from Sargassum fulvellum Ethanol Extract on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Cells

Jeong

Journal of Microbiology and Biotechnology

et al. 2019

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This study evaluated the anti-inflammatory potential of a grasshopper ketone (GK) isolated from the brown alga Sargassum fulvellum on lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophage cell line. GK was isolated and purified from the n-hexane fraction and its structure was verified on the basis of NMR spectroscopic data. GK up to 100 μg/ml is not cytotoxic to RAW 264.7, and is an effective inhibitor of LPS-induced NO production in RAW 264.7 cells. The production of pro-inflammatory cytokines, including IL-6, IL-1β, and TNF-α was found significantly reduced in 0.1-100 μg/ml dose ranges of GK treatment (p < 0.05). We confirmed the dose-dependent and significant inhibition of iNOS and COX-2 proteins expression. In addition, it has been shown that GK induces anti-inflammatory effects by inhibiting MAPKs (ERK, JNK, and p38) and NF-κB p65 phosphorylation. Our results show that the anti-inflammatory properties of GK may be due to the inhibition of the NF-κB and MAPKs pathways, which are associated with the attenuation of cytokine secretion.

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In vivo and in vitro inhibitory activity of an ethanolic extract of Sargassum fulvellum and its component grasshopper ketone on atopic dermatitis

International Immunopharmacology

et al. 2016