Anti-inflammatory effect of garlic 14-kDa protein on LPS-stimulated-J774A.1 macrophages

Rabe, Shahrzad Zamani Taghizadeh; Ghazanfari, Tooba; Siadat, Zahra Dana; Rastin, Maryam; Rabe, Shahin Zamani Taghizadeh; Mahmoudi, Mahmoud

doi:10.3109/08923973.2015.1005229

Cited by 20 publications

(7 citation statements)

References 29 publications

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“…Zamani Taghizadeh Rabe et al found that 14-kDa protein in allicin affected the expressions of inflammatory cytokines such as NO, TNF-α, and interleukin (IL) 1β by inhibiting LPS-stimulated J774A in the NF-κB signaling pathway. 63 This is also consistent with the anti-inflammatory, antibacterial, and vascular repair functions of the H 2 S donor allicin in this study.…”

Section: Discussionsupporting

confidence: 90%

Allicin-Loaded Intelligent Hydrogel Coating Improving Vascular Implant Performance

Han,

Lu,

Zou

et al. 2023

ACS Appl. Mater. Interfaces

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Coronary atherosclerosis is closely related to inflammation and oxidative stress. Owing to poor biocompatibility, lack of personalized treatment, and late toxic side effects, traditional drug-eluting stent intervention, releasing antiproliferative drugs, can delay endothelial repair and cause late thrombosis. The inflammation caused by atherosclerosis results in an acidic microenvironment and oxidative stress, which can be considered as triggers for precise and intelligent treatment. Here, we used catechol hyaluronic acid (C-HA) and cystamine (Cys) to prepare C-HA-Cys hydrogel coatings by amide reaction. The H 2 S-releasing donor allicin was loaded in the hydrogel to form an intelligent biomimetic coating. The disulfide bond of Cys made the crosslinked network redox-responsive to the inflammation and oxidative stress in the microenvironment by releasing the drug and H 2 S intelligently to combat the side effects of stent implantation. This study evaluated the hemocompatibility, anti-inflammatory capacity, vascular wall cytocompatibility, and in vivo histocompatibility of this intelligent hydrogel coating. Furthermore, the effect of H 2 S released from the coating on atherosclerosis-related signaling pathways such as CD31 and cystathionine γ-lyase (CSE), CD36, and ACAT-1 was investigated. Our results indicate that the C-HA-Cys-Allicin hydrogel coating could be manufactured on the surface of vascular interventional devices to achieve a precise response to the microenvironment of the lesion to release drug, which can attain the purpose of prevention of in-stent restenosis and ensure the effectiveness and safety of the application of interventional devices.

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Section: Discussionsupporting

confidence: 90%

Allicin-Loaded Intelligent Hydrogel Coating Improving Vascular Implant Performance

Han,

Lu,

Zou

et al. 2023

ACS Appl. Mater. Interfaces

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“…Kim et al (2017) claimed that after the treatment of RAW 264.7 cells with black A. sativum bulb-derived components, their cell viabilities did not significantly change [24]. A 14 kD protein derived A. sativum bulb had no impact on J774A.1 macrophage viability at 5-30 µg/mL [25]. According to Lee et al A. sativum bulbderived organosulfur compound of Diallyl Trisulfide (DATS) had no effect on murine RAW 264.7 macrophage cell viability at lower concentrations (10-30 µM) [26].…”

Section: Discussionmentioning

confidence: 99%

The Immunoregulatory Effects of Four Allium Species on Macrophages and Lymphocytes Viability

et al. 2018

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Background: Immune cells perform unique functions against infections and imbalances in immunity associated with various diseases. Applying natural products may control immune responses. Among herbals, much attention has been paid to the immunoregulatory functions of Allium sativum. However, the effects of other Allium species on the immune system have remained undiscovered. Therefore, the current study investigated the effects of A. sativum, A. Iranicum, A. elburzense, and A. asarense bulb extracts on macrophage and lymphocyte viability. Materials and Methods: The different concentrations of aqueous extracts of the aged bulb samples were prepared and used for the incubation of examined immune cells. Macrophages and lymphocytes were isolated and cultured in the presence of different concentrations of bulb extracts of Allium species. Macrophages and lymphocytes viability was followed by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. Results: The applied Allium species had stimulatory or inhibitory effects on the examined immune cells at different concentrations. Allium sativum demonstrated the highest effects on macrophage viability indices at 1 mg/mL. The similar effects were found for A. Iranicum extract on macrophage viability indices at 0.01 mg/mL. The bulb extract of A. sativum at the most used concentrations stimulated lymphocyte viability indices. The bulb extracts of all 4 Allium species inhibited viability indices of lymphocytes at 1 mg/mL. The bulb extracts of A. elburzense, at all of the applied concentrations, slightly affected viability indices of the macrophage and lymphocyte. Conclusion: Besides A. sativum, wild growing Allium species could be introduced to study their immunomodulatory effects on various diseases.

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“…In a study, the ethyl linoleate in garlic reduced the production of nitric oxide (NO) and prostaglandin E-2 by down-regulating the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX2) in lipopolysaccharide-stimulated RAW 264.7 macrophages [45]. Another study revealed that the garlic 14-kDa protein inhibited the inflammatory mediators including NO, TNF-α, and interleukin (IL) -1β by inhibiting the transcription factor nuclear factor-kappa B (NF-κB) signaling pathway in lipopolysaccharide-stimulated J774A.1 macrophages [46]. In addition, AGE inhibited inflammation in apolipoprotein E-knockout mice.…”

Section: Biological Functions Of Garlicmentioning

confidence: 99%

Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.)

Shang

Cao

et al. 2019

Foods

571

360

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Garlic (Allium sativum L.) is a widely consumed spice in the world. Garlic contains diverse bioactive compounds, such as allicin, alliin, diallyl sulfide, diallyl disulfide, diallyl trisulfide, ajoene, and S-allyl-cysteine. Substantial studies have shown that garlic and its bioactive constituents exhibit antioxidant, anti-inflammatory, antibacterial, antifungal, immunomodulatory, cardiovascular protective, anticancer, hepatoprotective, digestive system protective, anti-diabetic, anti-obesity, neuroprotective, and renal protective properties. In this review, the main bioactive compounds and important biological functions of garlic are summarized, highlighting and discussing the relevant mechanisms of actions. Overall, garlic is an excellent natural source of bioactive sulfur-containing compounds and has promising applications in the development of functional foods or nutraceuticals for the prevention and management of certain diseases.

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Anti-inflammatory effect of garlic 14-kDa protein on LPS-stimulated-J774A.1 macrophages

Cited by 20 publications

References 29 publications

Allicin-Loaded Intelligent Hydrogel Coating Improving Vascular Implant Performance

Allicin-Loaded Intelligent Hydrogel Coating Improving Vascular Implant Performance

The Immunoregulatory Effects of Four Allium Species on Macrophages and Lymphocytes Viability

Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.)

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