Anti-inflammatory Effect of Glycoprotein Isolated fromCudrania TricuspidataBureau: Involvement of MAPK/NF-κB Signaling

Abstract

The aim of this study was to evaluate inhibitory effect of glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) on allergic responses. We evaluated the activities of mitogen-activated protein kinase (MAPK), transcriptional factor, and production of immunoglobulin (Ig)E and interleukin (IL)-4 in RBL-2H3 cells and BALB/c mice. Our results showed that CTB glycoprotein inhibited the production of IgE and IL-4 in serum from ovalbumin (OVA)-treated BALB/c mice. We also found that CTB glycoprotein inhibited the phosphorylation of p38 MAPK, and transcriptional activation of nuclear factor (NF)-κB in RBL-2H3 cells. The activation of NF-κB was effectively blocked by treatment with p38 MAPK inhibitor (SKF86002). The results from these experiments indicate that the CTB glycoprotein inhibits release of β-hexosaminidase, and production of IgE and IL-4 via down regulation of MAPK/ NF-κB on the stage of mast cell degranulation. In conclusion, we suggest that the CTB glycoprotein might be a potent preventive agent in allergic responses.

Application of Cudrania tricuspidata leaf extract as a washing agent to inactivate Listeria monocytogenes on fresh‐cut romaine lettuce and kale

“…Cudrania tricuspidata of the Mulberry family is cultivated in Korea, China, Japan and Russia (Song et al, 2017), and it is commonly called silkworm thorn (Kim & Lee, 2015). Anthocyanin, flavonoids and polyphenols present in the leaves, berries and roots of Cudrania tricuspidata are known to have pharmacological functions, such as anti-inflammation (Oh & Lim, 2011), anti-obesity (Jo et al, 2015) and antioxidant activities (Lee et al, 2009). However, studies on the antimicrobial activity of Cudrania tricuspidata leaf extract (CTLE) have not been conducted yet, and CTLE has never been studied as a washing agent for fresh-cut vegetables.…”