Anti-inflammatory effect of lentil hull (Lens culinaris) extract via MAPK/NF-κB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture

P, Li; Guo, Fanghua; Pei, Minjia; Tsao, Rong; Wang, Xiaoya; Jiang, Li; Sun, Yong; Xiong, Hua

doi:10.1016/j.jff.2022.105044

Cited by 23 publications

(16 citation statements)

References 40 publications

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“…60 Previous studies showed that chronic inflammation upregulates iNOS and COX-2 enzymes, precursors for NO and PGE2 synthesis, two pro-inflammatory factors. 61,62 In the current study, the inflammatory challenge increased the pro-inflammatory cytokines and chemokines (iNOS, COX-2, IL-8, and TNF-α) mRNA levels in the Caco-2 cells. Similar results were found elsewhere.…”

Section: Food and Function Papermentioning

confidence: 52%

“…The inhibition of the NF-kB pathway is recognized as a potential strategy to reduce intestinal inflammation. 46,62,73 Previous studies have shown that plant-derived polysaccharides alleviate gut inflammation by modulating the intestinal immune status through inhibition of the NF-kB pathway. 3,74,75 In the current study, SMSP2 treatment reduced NF-kBp65 protein phosphorylation in the Caco-2 cells.…”

Section: Food and Function Papermentioning

confidence: 99%

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Senegalia macrostachya seed polysaccharides attenuate inflammation-induced intestinal epithelial barrier dysfunction in a Caco-2 and RAW264.7 macrophage co-culture model by inhibiting the NF-κB/MLCK pathway

et al. 2022

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Section: Food and Function Papermentioning

confidence: 52%

Section: Food and Function Papermentioning

confidence: 99%

Senegalia macrostachya seed polysaccharides attenuate inflammation-induced intestinal epithelial barrier dysfunction in a Caco-2 and RAW264.7 macrophage co-culture model by inhibiting the NF-κB/MLCK pathway

et al. 2022

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“…The role of polyphenols in the anti-inflammatory capacity of lentil hulls was reported also by Peng et al, by testing their digestive products on the Caco-2 cell monolayer and Caco-2/RAW264.7. In this case, the protocatechuic acid glycoside derivative was found to have the highest content in the lentil hulls digestive product [ 54 ]. These investigations suggest the key role of the phenolics that are contained in the lentil hulls on the beneficial effects that are exerted on biological systems.…”

Section: Resultsmentioning

confidence: 99%

Microwave-Assisted Extraction of Bioactive Compounds from Lentil Wastes: Antioxidant Activity Evaluation and Metabolomic Characterization

et al. 2022

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The recovery of industrial by-products is part of the zero-waste circular economy. Lentil seed coats are generally considered to be a waste by-product. However, this low-value by-product is rich in bioactive compounds and may be considered an eco-friendly source of health-promoting phytochemicals. For the first time, a sustainable microwave-assisted extraction technique was applied, and a solvent screening was carried out to enhance the bioactive compound content and the antioxidant activity of green and red lentil hull extracts. With respect to green lentil hull extracts that were obtained with different solvents, the aqueous extract of the red lentil seed coats showed the highest total phenolic and total flavonoid content (TPC = 28.3 ± 0.1 mg GAE/g dry weight, TFC = 1.89 ± 0.01 mg CE/100 mg dry weight, respectively), as well as the highest antioxidant activity, both in terms of the free radical scavenging activity (ABTS, 39.06 ± 0.73 mg TE/g dry weight; DPPH, IC50 = 0.39 μg/mL) and the protection of the neuroblastoma cell line (SH-SY5Y, IC50 = 10.1 ± 0.6 μg/mL), the latter of which has never been investigated so far. Furthermore, a metabolite discovery analysis was for the first time performed on the aqueous extracts of both cultivars using an HPLC separation which was coupled with an Orbitrap-based high-Resolution Mass Spectrometry technique.

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“…The method of the in vitro digestion process used was as described by Brodkorb et al 25 Simulated digestive fluids for the oral, gastric, and intestinal stages were prepared before the experiment began and the detailed parameters are as shown in our previous study. 26 Air was purged with nitrogen before each stage of digestion. All experiments were performed under dark conditions.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Free and Bound Phenolics of Laird Lentil (Lens culinaris) Hulls and the Anti-inflammatory Activity of their Digestive Products via Crosstalk between NF-κB and Keap1-Nrf2 Signaling Pathways in HT-29 Cells

Guo

Xiong

et al. 2022

J. Agric. Food Chem.

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Polyphenol-rich Laird lentil hulls are a byproduct of lentil processing. In the present study, free and bound polyphenols in lentil hulls were analyzed with UHPLC-LTQ-OrbiTrap-MS2, and the anti-inflammatory mechanism of their digestive products was explored based on the NF-κB and Keap1-Nrf2 signaling pathways in the HT-29 cell model. In summary, a total of 27 polyphenols and 5 nonphenolic constituents were identified in free and bound fractions, and among them, catechin glucoside, kaempferol tetraglucoside, procyanidin dimer, and dihydroxybenzoic acid-O-dipentoside were the main polyphenols in the digestive products. These digestive products could reduce inflammatory mediators and exert anti-inflammatory activity by inhibiting NF-κB and activating Keap1-Nrf2 signaling pathways, and there was crosstalk between them, which was a mutual inhibition effect. The results show that polyphenols in lentil hulls are a good source of anti-inflammatory ingredients and have a promising development potential.

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Anti-inflammatory effect of lentil hull (Lens culinaris) extract via MAPK/NF-κB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture

Cited by 23 publications

References 40 publications

Senegalia macrostachya seed polysaccharides attenuate inflammation-induced intestinal epithelial barrier dysfunction in a Caco-2 and RAW264.7 macrophage co-culture model by inhibiting the NF-κB/MLCK pathway

Senegalia macrostachya seed polysaccharides attenuate inflammation-induced intestinal epithelial barrier dysfunction in a Caco-2 and RAW264.7 macrophage co-culture model by inhibiting the NF-κB/MLCK pathway

Microwave-Assisted Extraction of Bioactive Compounds from Lentil Wastes: Antioxidant Activity Evaluation and Metabolomic Characterization

Free and Bound Phenolics of Laird Lentil (Lens culinaris) Hulls and the Anti-inflammatory Activity of their Digestive Products via Crosstalk between NF-κB and Keap1-Nrf2 Signaling Pathways in HT-29 Cells

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