Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema

Tae, Jin; Han, Sung-Hyun; Yoo, Jae Young; Kim, Jina; Kang, Ok‐Hwa; Baek, Ok-Seon; Lim, Jong-Pil; Kim, Dae‐Ki; Kim, Young‐Ho; Bae, KiHwan; Lee, Young-Mi

doi:10.1016/s0009-8981(03)00017-2

Cited by 48 publications

(32 citation statements)

References 25 publications

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“…Fresh DPPH stock solution was prepared by dissolving DPPH in methanol on each day of analysis and kept at 48C, in a volumetric flask protected from light. Reference compounds, including chlorogenic acid (1), caffeic acid (4), sweroside (6), rutin (7), isoquercitrin (8), luteolin-7-O-glucoside (9), lonicerin (10), 3,5-O-dicaffeoylquinic acid (diCQA) (12), 3,4-O-diCQA (14), centauroside (15), and luteolin (17) (Fig. 1), were isolated from several species of Lonicera in our laboratory and their identities were confirmed by UV, IR, 1 H-and 13 C-NMR, and MS analysis [21 -25].…”

Section: Reagents and Chemicalsmentioning

confidence: 99%

“…The reported antioxidant properties of the components might also be responsible for some medicinal claims such as the anti-inflammatory activities that are associated with free radicals [32]. In addition, the quantitative analysis of some components (compounds 1, 2, 4 -5, 11 -14, and 16) in different FLJ extracts were carried out according to the method described in the literature [19], and the results (listed in Table 3) showed that 3-CQA (1), caffeic acid (4), 3,5-diCQA (12) and 3,4-diCQA (14), were the major natural antioxidants in FLJ extract. Compounds 6 and 15, with no activity to DPPH free radicals, could also be unequivocally identified as sweroside and centauroside by comparison with the reference compounds under identical analytical conditions, respectively.…”

Section: Identification Of Free Radical Scavenging Components In the mentioning

confidence: 99%

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Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH‐HPLC‐DAD‐TOF/MS

Tang

Chen

et al. 2008

J of Separation Science

114

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A rapid and simple method has been developed for the screening and identification of natural antioxidants of Flos Lonicerae Japonicae (FLJ), derived from the flower buds of Lonicera japonica. The hypothesis is that upon reaction with 1,1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas (PAs) of compounds with potential antioxidant effects in the HPLC chromatograms will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS hyphenated technique. Using the proposed approach, about 14 compounds in the FLJ extract were found to possess a potential antioxidant activity. They were identified as chlorogenic acid (1), 1-O-caffeoylquinic acid (1-O-CQA, 2), caffeic acid (4), 4-O-CQA (5), rutin (7), isoquercitrin (8), luteolin-7-O-glucoside (9), lonicerin (10), 4,5-O-dicaffeoylquinic acid (4,5-O-diCQA, 11), 3,5-O-diCQA (12), 1,3-O-diCQA (13), 3,4-O-diCQA (14), 1,4-O-diCQA (16), and luteolin (17). In addition, the free radical scavenging capacities of the available identified compounds were also investigated by HPLC assay. The results indicated that the compounds with PAs significantly decreasing were natural antioxidants, whereas those with PAs not changing presented no activities, which accordingly indicated that this newly proposed method could be widely applied for rapid screening and identification of natural antioxidants from complex matrices including Chinese herbal medicines.

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Section: Reagents and Chemicalsmentioning

confidence: 99%

Section: Identification Of Free Radical Scavenging Components In the mentioning

confidence: 99%

Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH‐HPLC‐DAD‐TOF/MS

Tang

Chen

et al. 2008

J of Separation Science

114

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“…Previous studies have shown that LJ could act as an anti-inflammatory agent through regulation of NF-B activation, since it inhibits the appearance of nuclear NF-B p65 and the action of I-B in the liver of lipopolysaccharide (LPS) challenged rats (Lee et al, 2001). LJ has also been found to inhibit LPS induced inflammatory responses such as induction of tumor necrosis factor (TNF-␣) and inducible nitric oxide synthase as well as by inhibiting PAR2 agonist induced myeloperoxidase activity in paw tissue (Tae et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Trifunctional inhibition of COX-2 by extracts of Lonicera japonica: Direct inhibition, transcriptional and post-transcriptional down regulation

Oliverson

Simmons

2007

Journal of Ethnopharmacology

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“…is a safe and mild antiinflammatory agent for treating various inflammatory disorders. Its different extracts can inhibit arachidonic acid ear oedema, rat cotton pellet granuloma, and pro-D E inflammatory cytokines expression (12,29) in inflammatory model in vitro or in vivo. These findings suggest that JYT has potential benefits for TLR4 signalling of LPS-induced MECs.…”

Section: Discussionmentioning

confidence: 99%

Chinese herbal Jin-Ying-Tang attenuates the inflammatory response by inhibiting the activation of TLR4/MyD88/TRAF-6/NIK pathway at the mRNA level in LPS-stimulated mouse mammary epithelial cells

Qin¹,

et al. 2016

Journal of Veterinary Research

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Introduction: The effects of Jin-Ying-Tang (JYT) on Toll-like Receptor 4 (TLR4) signalling transduction of lipopolysaccharide (LPS)-stimulated mouse mammary epithelial cells (MECs) in vitro were examined. Material and Methods: The cytotoxicity of JYT (0.06-62.50 mg/mL) on mouse MECs was determined by MTT assay. The MECs were co-cultured with LPS in the presence or absence of JYT (39.10 μg/mL, 391 μg/mL, 3910 μg/mL). The concentrations of interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) in the culture supernatants were detected by ELISA. The mRNA expression of TLR4 and downstream TLR4 signalling molecules such as myeloid differentiation factor 88 (MyD88), tumour necrosis factor receptor associated factor 6 (TRAF-6), inhibitor κB (IκB), and nuclear factor κB inducing kinase (NIK) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Results: The results showed that the IC50 of JYT on MECs was 12.25 mg/mL and JYT could significantly decrease the concentrations of IL-6 and TNF-α in LPS-stimulated MECs (P < 0.05). The mRNA expression of TLR4, MyD88, TRAF-6, IκB, and NIK was also significantly decreased when the LPS-stimulated MECs were cocultured at appropriate concentrations of JYT (P < 0.05, P < 0.01). Conclusion: These observations indicate a potential mechanism through which JYT attenuates the systemic inflammatory response to LPS-stimulated mouse mammary epithelial cells by inhibiting the activation of TLR4/MyD88/ TRAF-6/NIK pathway at the mRNA level.

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Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema

Cited by 48 publications

References 25 publications

Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH‐HPLC‐DAD‐TOF/MS

Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH‐HPLC‐DAD‐TOF/MS

Trifunctional inhibition of COX-2 by extracts of Lonicera japonica: Direct inhibition, transcriptional and post-transcriptional down regulation

Chinese herbal Jin-Ying-Tang attenuates the inflammatory response by inhibiting the activation of TLR4/MyD88/TRAF-6/NIK pathway at the mRNA level in LPS-stimulated mouse mammary epithelial cells

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