Anti-Inflammatory Effect of Unsaturated Fatty Acids and Ergosta-7,22-dien-3-ol from Marthasterias glacialis: Prevention of CHOP-Mediated ER-Stress and NF-κB Activation

Pereira, David M.; Correia-da-Silva, Georgina; Valentão, Patrı́cia; Teixeira, Natércia; Andrade, Paula B.

doi:10.1371/journal.pone.0088341

Cited by 63 publications

(45 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In fact, marine organisms present a huge diversity and live in complex habitats exposed to extreme conditions becoming, in this way, a unique source of bioactive compounds, which cannot be found in other organisms. 3 Among them we can highlight the presence of long-chain polyunsaturated fatty acids (PUFA), especially those with three or more double bonds, which are a class of lipids characteristic of marine organism with important bioactivities. 2 For example, research developed by our group showed that the echinoderm Marthasterias glacialis (spiny sea-star) contains a big amount of fatty acids, being an interesting source of anti-inammatory molecules.…”

Section: Introductionmentioning

confidence: 99%

Fatty acids from edible sea hares: anti-inflammatory capacity in LPS-stimulated RAW 264.7 cells involves iNOS modulation

et al. 2015

View full text Add to dashboard Cite

The inclusion of marine organisms in the diet is gaining importance due to their richness in health beneficial nutrients. This study evaluated the fatty acid composition and anti-inflammatory potential of lipophilic extracts of two edible sea hares, Aplysia fasciata Poiret and Aplysia punctata Cuvier. Twenty-five fatty acids were determined, nine of them not yet reported in these species. They revealed similar antiinflammatory properties in RAW 264.7 cells stimulated with lipopolysaccharide, as ascertained by the decreased levels of cNO in the culture medium. A decrease of L-citrulline was also observed, indicating that the compounds may act by modulation of inducible nitric oxide synthase (iNOS). A. punctata was most effective as lipoxygenase inhibitor, probably because it contains more polyunsaturated fatty acids (PUFA) that can compete with linoleic acid for the active site, decreasing enzyme activity. The antiinflammatory potential of A. fasciata and A. punctata is reported here for the first time.

show abstract

Section: Introductionmentioning

confidence: 99%

Fatty acids from edible sea hares: anti-inflammatory capacity in LPS-stimulated RAW 264.7 cells involves iNOS modulation

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Marine-derived molecules have been a prolific source of new anti-inflammatory drugs [11,12,13], reason for which we have conducted screening studies on the anti-inflammatory activity of the aqueous extracts of both sea anemones and their major metabolite, the alkaloid homarine.…”

Section: Resultsmentioning

confidence: 99%

“…In this situation, macrophages present a number of biochemical traits, such as increased production of NO via inducible nitric oxide synthase (iNOS) [11] and increased levels of intracellular ROS.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Anti-Inflammatory and Cytotoxic Effects of Aqueous Extracts from the Edible Sea Anemones Anemonia sulcata and Actinia equina

Silva

Andrade

Paiva‐Martins

et al. 2017

IJMS

Self Cite

View full text Add to dashboard Cite

Marine invertebrates have been attracting the attention of researchers for their application in nutrition, agriculture, and the pharmaceutical industry, among others. Concerning sea anemones (Cnidaria), little is known regarding their metabolic profiles and potential value as a source of pharmacologically-active agents. In this work, the chemical profiles of two species of sea anemones Actinia equina and Anemonia sulcata, were studied by high-performance liquid chromatography with diode-array detection (HPLC-DAD) and its impact upon immune and gastric cells was evaluated. In both species, the methylpyridinium alkaloid homarine was the major compound in aqueous extracts. The extracts were effective in reducing lipopolysaccharide (LPS)-induced levels of nitric oxide (NO) and intracellular reactive oxygen species (ROS) in a macrophage model of inflammation. Both the extracts and the alkaloid homarine were effective in inhibiting phospholipase A 2 (PLA 2 ), a pivotal enzyme in the initial steps of the inflammatory cascade. In order to mimic the oral consumption of these extracts; their effect upon human gastric cells was evaluated. While no caspase-9 activation was detected, the fact that the endoplasmic reticulum-resident caspase-4, and also caspase-3, were activated points to a non-classical mechanism of apoptosis in human gastric cells. This work provides new insights on the toxicity and biological potential of sea anemones increasingly present in human nutrition.

show abstract

“…Cell viability was evaluated by the MTT reduction assay . After incubation (24 h), MTT (final concentration 0.5 mg/ml) was added to each well and the plate was incubated for 2 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Exploratory Studies on the in Vitro Anti‐inflammatory Potential of Two Herbal Teas (Annona muricata L. and Jasminum grandiflorum L.), and Relation with Their Phenolic Composition

Oliveira

Sá

Pereira

et al. 2017

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

The need of new anti-inflammatory drugs has led to the search for safer and more potent molecules in distinct sources, such as natural products. This work aimed to explore the anti-inflammatory potential of aqueous extracts from two herbal teas (Annona muricata L. and Jasminum grandiflorum L.) in RAW 264.7 macrophages cells and in cell-free assays. Furthermore, the phenolic composition of both extracts and of their hydrolysates was characterized by HPLC-DAD, in order to establish possible relationships with the biological activity. In a general way, A. muricata displayed a stronger capacity to inhibit nitric oxide (NO) production and the activity of phospholipase A (PLA ), displaying an IC value of 142 μg/ml against this enzyme. A deeper look at phenolic compounds revealed that aglycones had more capacity to inhibit NO and PLA than their corresponding glycosides, quercetin being clearly the most potent one (IC = 7.47 and 1.36 μm, respectively). In addition, 5-O-caffeoylquinic acid, at 1.56 μm, could also inhibit PLA (ca. 35%). Our findings suggest that the consumption of both herbal teas may be a preventive approach to inflammatory disorders.

show abstract

Anti-Inflammatory Effect of Unsaturated Fatty Acids and Ergosta-7,22-dien-3-ol from Marthasterias glacialis: Prevention of CHOP-Mediated ER-Stress and NF-κB Activation

Cited by 63 publications

References 24 publications

Fatty acids from edible sea hares: anti-inflammatory capacity in LPS-stimulated RAW 264.7 cells involves iNOS modulation

Fatty acids from edible sea hares: anti-inflammatory capacity in LPS-stimulated RAW 264.7 cells involves iNOS modulation

In Vitro Anti-Inflammatory and Cytotoxic Effects of Aqueous Extracts from the Edible Sea Anemones Anemonia sulcata and Actinia equina

Exploratory Studies on the in Vitro Anti‐inflammatory Potential of Two Herbal Teas (Annona muricata L. and Jasminum grandiflorum L.), and Relation with Their Phenolic Composition

Contact Info

Product

Resources

About