Anti-inflammatory Effect of Zostera marina Ethanolic Extract on LPS-induced RAW264.7 Cells and Mouse Model

Abstract

The Zostera marina ethanolic extract (ZMEE) was tested in this study to investigate the anti-inflammatory activity in LPS-induced RAW 264.7 cells and mouse model. Nitric oxide production and inducible nitiric oxide synthase expression in cells treated with ZMEE was reduced significantly in a dose-dependent manner. Similarly, the secretion of pro-inflammatory cytokines such as interleukin (IL)-6, IL-1β, and TNF-α was inhibited markedly. In addition, the expression of nuclear factor kappa B (NF-κB) and the phosphorylation of JNK, ERK, and p38 MAPKs was suppressed by ZMEE as well. In vivo test, ZMEE attenuated the croton oil-induced mouse ear edema and there were no mortalities in mice administered 5,000 mg/kg body weight of ZMEE during the observation periods. The results in photomicrograph of mice ear tissue showed the reduction of dermal thickness and the number of infiltrated mast cells. These results indicate that ZMEE inhibits the production of LPS-induced pro-inflammatory mediators, suggesting that ZMEE may be a potential material for anti-inflammatory therapies.

Anti-Inflammatory Effects of Monostroma crassidermum Tokida Ethanol Extract on Lipopolysaccharide-Stimulated RAW 264.7 Cells and Mice Ear Edema

“…To confirm the anti-inflammatory effect of MCEE in the mouseear edema model, ear edema measurements and histopathological analyses were conducted using the methods of Kim et al [15] and Saraiva et al [16]. MCEE (200 μL) was orally administered to ICR mice at 10, 50 and 250 mg/kg body weight.…”

Anti-Inflammatory Effect of Chondrus ocellatus Holmes Ethanol Extract on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells

“…한편, 해조류는 일상생활에서 섭취 가능한 천연물의 하나 로 항산화 [29], 고지혈증 개선 [22], 항아토피 [11], 콜레스테롤 침착 방지 [15], 항균 [27], 항염증 [16] 관 생성을 유도하는 데 관여한다 [7]. NF-κB는 염증반응과 관 련된 유전자의 promoter에 결합하여 iNOS, COX-2의 발현 에 관여하는 것으로 알려져 있다 [5].…”

Anti-Inflammatory Effects of Ethanol Extracts from Ulva pertusa Kjellman on LPS-induced RAW 264.7 Cells and Mouse Model

“…MAPKs의 활성 은 대식세포에서 일어나며 extra cellular signal-regulated kinase (ERK), c-jun NH 2 -terminal kinase (JNK), p-38과 같은 주요 3개의 인자가 존재한다. 이러한 signaling pathway 의 활성을 억제함으로써 염증성 cytokine의 분비량을 억제 하고자 하며 유효물질을 천연물로부터 확인하고자 현재 많 은 관련 연구들이 진행되고 있다 [10]. 본 연구에서 사용된 구 멍갈파래(Ulva pertusa Kjellman)는 녹조류의 하나로 단독 혹은 2−3가닥으로 뭉쳐서 자라며 10−30 cm 또는 그 이상에 달한다.…”

“…구멍갈파래는 특히 황산기를 함유한 다당을 다량 함유하고 있어 이로 인한 항 종양성 [23], 항 바이러스성 [2], 면역 증진의 효과 [20], 혈액의 항 응고작 용 [17] 비를 촉진한다 [12]. 결과를 보였으며 [10]…”