Anti-Inflammatory Effects of an Extract from Pseudomonas aeruginosa and Its Purified Product 1-Hydroxyphenazine on RAW264.7 Cells

Xiao, Jun; Thwe, Aye Aye; Liu, Tingting; Gong, Dafei; Lin, Wenchu; Shang, Changhua; Lu, Zujun

doi:10.1007/s00284-021-02544-3

Cited by 6 publications

(4 citation statements)

References 44 publications

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“…It has been reported that HPC has potential medicinal effects, such as anti-cancer activity against cell lines 1321N1 and A549 [ 54 , 55 ], antibacterial activity against human pathogenic bacterial strains Candida albicans , Aspergillus fumigatus , Escherichia coli , Xanthomonas campestris pv. vesicatoria [ 55 , 56 , 57 ], and anti-inflammatory activities [ 58 ]. HPC has also reportedly been used in agriculture against various fungal and bacterial strains [ 53 , 59 ].…”

Section: Resultsmentioning

confidence: 99%

Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards

et al. 2022

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α-Amylase inhibitors (aAIs) have been applied for the efficient management of type 2 diabetes. The aim of this study was to search for potential aAIs produced by microbial fermentation. Among various bacterial strains, Pseudomonas aeruginosa TUN03 was found to be a potential aAI-producing strain, and shrimp heads powder (SHP) was screened as the most suitable C/N source for fermentation. P. aeruginosa TUN03 exhibited the highest aAIs productivity (3100 U/mL) in the medium containing 1.5% SHP with an initial pH of 7–7.5, and fermentation was performed at 27.5 °C for two days. Further, aAI compounds were investigated for scaled-up production in a 14 L-bioreactor system. The results revealed a high yield (4200 U/mL) in a much shorter fermentation time (12 h) compared to fermentation in flasks. Bioactivity-guided purification resulted in the isolation of one major target compound, identified as hemi-pyocyanin (HPC) via gas chromatography-mass spectrometry and nuclear magnetic resonance. Its purity was analyzed by high-performance liquid chromatography. HPC demonstrated potent α-amylase inhibitory activity comparable to that of acarbose, a commercial antidiabetic drug. Notably, HPC was determined as a new aAI. The docking study indicated that HPC inhibits α-amylase by binding to amino acid Arg421 at the biding site on enzyme α-amylase with good binding energy (−9.3 kcal/mol) and creating two linkages of H-acceptors.

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Section: Resultsmentioning

confidence: 99%

Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards

et al. 2022

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“…However, previous research shows that secondary metabolites of Pseudomonas such as indole, amino acids, and peptides have significant biological activity, including antiinflammatory activity. For example, in vitro studies of macrophage cell lines showed that supernatants from P. aeroginosa culture broth significantly reduced the release of TNF- , one of the components of IF5, and its mRNA expression level and inhibited the LPS-induced polarization of macrophages ( 40 , 41 ). In addition, the study by Khan et al ( 41 ) reported a decreased plasma level of TNF-α with oral administration of the bacterial supernatant as well as a proline-based cyclic dipeptide, a compound identified in the bacterial supernatant, to rats followed by LPS injection ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

Association of intestinal bacteria with immune activation in a cohort of healthy adults

Riazati,

Kable,

Stephensen

2023

Microbiol Spectr

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Interactions among intestinal bacteria and the immune system contribute to the maintenance of a functional intestinal barrier in healthy individuals, and possibly to systemic immune activity. We hypothesized that intestinal bacteria would be associated with systemic biomarkers of innate and adaptive immune responses in healthy adults. 79 immune function markers were subjected to factor analysis resulting in 17 Immune Factors (IFs), each composed of 2–10 immune variables. Bacterial taxa from stool samples were identified at the family and genus levels by 16S rRNA amplicon sequence analysis and their read counts and relative abundances were utilized in a multiple linear regression model to identify microbial taxa associated with the IFs. A total of 10 significant associations were identified between bacterial taxa and IFs. The family Rikenellaceae showed a positive association with innate IF5 (including 5 chemokines, 2 cytokines, 2 adhesion molecules, and the macrophage metabolite neopterin) and a negative association with adaptive IF4 (including T-cells with activation marker HLA-DR). Additionally, Pseudomonadaceae and its genus Pseudomonas showed a negative relationship with innate IF5, and adaptive IF13 (including T-cell cytokines IL-10, IL-17, and IFN-γ) was negatively associated with Butyrivibrio and positively associated with Slackia . These associations suggest ongoing interactions between gut bacteria and the systemic immune system in healthy adults. The association of these taxa with the IFs may result from specific microbial-immune system interactions that play a role in maintenance of a healthy barrier integrity in our cohort of healthy adults. IMPORTANCE Chronic inflammation may develop over time in healthy adults as a result of a variety of factors, such as poor diet directly affecting the composition of the intestinal microbiome, or by causing obesity, which may also affect the intestinal microbiome. These effects may trigger the activation of an immune response that could eventually lead to an inflammation-related disease, such as colon cancer. Before disease develops it may be possible to identify subclinical inflammation or immune activation attributable to specific intestinal bacteria normally found in the gut that could result in future adverse health impacts. In the present study, we examined a group of healthy men and women across a wide age range with and without obesity to determine which bacteria were associated with particular types of immune activation to identify potential preclinical markers of inflammatory disease risk. Several associations were found that may help develop dietary interventions to lower disease risk.

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“…Among the phenazine compounds biosynthesized by P. aeruginosa, numerous studies have reported the bioproduction of pyocyanin and its various bioactivities; however, there have only been a few reports on the production and bioactivities of HPC [31,[50][51][52][53][54][55]. To date, HPC has been reported to have some bio-effects, such as anti-cancer [51,52], antimicrobial [31,[51][52][53][54], and anti-inflammatory effects [55]. Thus, the novel α-glucosidase inhibitory activity of HPC reported in this work may enrich the catalog of the biological activities of HPC.…”

Section: The Assessment Of α-Glucosidase Inhibitory Effectsmentioning

confidence: 99%

Bioconversion of a Peanut Oil Processing By-Product into a Novel α-Glucosidase Inhibitor: Hemi-Pyocyanin

2023

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Hemi-pyocyanin (HPC) is a heterocyclic nitrogenous compound with some reported potential medical effects. The current report aimed to investigate the potential use of organic industrial waste for the production of HPC via microbial fermentation. The novel antidiabetic activity of HPC was also accessed and reported in this work. A peanut oil processing by-product (groundnut cake) was screened as the best substrate for Pseudomonas aeruginosa TUN03 conversion to obtain high-yield HPC. This compound was further produced in a 14 L bioreactor system on a large scale (6 L per pilot) and reached higher productivity (35.1 μg/mL) in a shorter time course of cultivation (8 h) compared to fermentation on a small scale in flasks (19.5 μg/mL; 3 days of fermentation). On assessing its activity, HPC demonstrated potent inhibition against α-glucosidase, an antidiabetic enzyme, with a low IC50 value (0.572 mg/mL) and a maximum inhibition rate of 100%. In an in silico study, HPC was found to inhibit α-glucosidase with a good binding energy score (−9.0 kcal/mol) via interaction with amino acids Lys156, Leu313, and Arg315 at the active site, and three bonds (1 H-acceptor and 2 pi-H) were generated. The data from five Lipkin’s rules and ADMET-based pharmacokinetics and pharmacology revealed that HPC possesses drug-like properties and good ADMET properties within the required allotted limitations. The data obtained in the current work highlighted the potential application of groundnut cakes for the eco-friendly and scaled-up production of HPC, a new anti-α-glucosidase agent that should be further investigated for type 2 diabetes management.

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Anti-Inflammatory Effects of an Extract from Pseudomonas aeruginosa and Its Purified Product 1-Hydroxyphenazine on RAW264.7 Cells

Cited by 6 publications

References 44 publications

Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards

Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards

Association of intestinal bacteria with immune activation in a cohort of healthy adults

Bioconversion of a Peanut Oil Processing By-Product into a Novel α-Glucosidase Inhibitor: Hemi-Pyocyanin

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