Anti-inflammatory effects of benfotiamine are mediated through the regulation of the arachidonic acid pathway in macrophages

Mohammed, Sabira; Ramana, Kota V.

doi:10.1016/j.freeradbiomed.2011.10.444

Cited by 58 publications

(46 citation statements)

References 58 publications

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“…Besides the central effects in lowering nociception, thiamine may reduce the inflammatory pain by lowering inflammation as it has been suggested that anti-inflammatory effect of this vitamin may be mediated through regulation of arachidonic acid pathway in macrophage, where it can block the expression of any of the enzymes (cycloxygenase-2, lipoxygenase-2, thromboxane B synthase, prostacycline synthase) as well as transcription factor nuclear factor kappa 54 . Moreover, this vitamin may also decrease the oxidative stress and production of free radicals 55 .…”

Section: Discussionmentioning

confidence: 99%

Analgesic Effects of Thiamine in Male Long Evans Rats

Ainan

Begum

Ali

2017

J Bangladesh Soc Physiol

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Background: The concept of analgesic effects of thiamine along with other B vitamins has been supported since long by various clinical and experimental evidences, though effects of individual thiamine on pain are yet to be clearly demonstrated. Objective: To assess the effects of increasing doses of thiamine supplementation on pain. Methods: Forty-eight (48) male Long Evans rats (200±20 gm) were given thiamine (100, 200, 250, mg/kg/day; experimental) or normal saline (5 ml/kg/day; control) intraperitonealy (i.p) for 7 consecutive days. The analgesic activity was evaluated by three experimental pain models, hot (52±0.5 0 C) water tail immersion test, the interphase (6 th -15 th minutes) of formalin (50 µl, 2.5%, subcutaneous) test and acetic acid (2%, i.p) induced writhing test. Statistical analysis was done by ANOVA followed by Bonferroni post hoc test and p≤0.05 was considered as significant. Results: In tail immersion test, %MPE significantly increased after 200 (p≤0.05) and 250 (p≤0.001) mg/kg of thiamine. In the formalin test, thiamine significantly lowered the jerking frequency (p≤0.05, p≤0.001, p≤0.001, respectively) and duration of flexing and licking (p≤0.001, in all doses), compared to control. In addition, in writhing test, significant increment in latency of appearance of 1 st writhe (p≤0.001, in higher 2 doses) and significant decrement in frequency of writhes (p≤0.01, p≥0.001, p≤0.001, respectively, in all doses) were observed. Conclusion: The results of this study conclude that, repetitive administration of thiamine may cause alleviation of pain through central as well as peripheral inhibitory mechanisms, which is dose dependent as well.

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Section: Discussionmentioning

confidence: 99%

Analgesic Effects of Thiamine in Male Long Evans Rats

Ainan

Begum

Ali

2017

J Bangladesh Soc Physiol

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“…Moreover, thiamine supplementation may also increase the availability or effectiveness of serotonin as well as noradrenaline in the CNS 24,26,27 . Besides the central effects in lowering nociception, it has been proposed that peripheral anti-inflammatory effect of this vitamin may be mediated through regulation of arachidonic acid pathway in macrophage, where it can block the expression of any of the enzymes (cycloxygenase-2, lipoxygenase-2, thromboxane B synthase, prostacycline synthase) as well as transcription factor nuclear factor kappa 28 . In addition, this vitamin may also decrease the oxidative stress and production of free radicals 29 .…”

Section: Discussionmentioning

confidence: 99%

“…Here, subcutaneous injection of formalin at hindpaw induces inflammation, which leads to a response characterized by jerking, flexing followed by licking of the affected hindlimb 23,25,26 . Pain intensity is measured by converting these behavioral responses into numerical values 24,25,26,27, assessed in early phase (first 5-7 minutes, results from direct chemical stimulation of the nociceptive afferent fibers mainly C fibers) 28 , while the late phase (last 40-45 minutes, results from the action of locally released inflammatory mediators and also by the facilitation of synaptic transmission in thespinalcord) 29 .…”

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confidence: 99%

Antinociceptive and anti-inflammatory effects of thiamine in Long Evans rats

Ainan

Begum

Ali

2016

J Bangladesh Soc Physiol

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Background: Thiamine along with other B vitamins has been prescribed since long for treatment of various painful conditions, though individual effects of thiamine on nociception and inflammation are yet to be clearly demonstrated. Objective: To assess the effects of increasing doses of thiamine supplementation against pain and inflammation. Methods: This experimental study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from March 2014 to February 2015. Total twenty four male Long Evans rats weighing (200±20 gm) were treated with three different doses of thiamine hydrochloride (THCL 100, 200, 250, mg/kg/day; experimental) or normal saline (5 ml/kg/day; control) intraperitoneally for 7 consecutive days. To evaluate the thiamine's effect on nociceptive pain, early phase (0-5 minutes) and on inflammatory pain, late phase (16-60 minutes) of the formalin test, were observed. In both phases, total frequency of jerking and total duration of flexing and licking of the right hind paw were counted after administration of subcutaneous formalin (50 µl, 2.5%) injection. After formalin test, all the rats were also subjected to formalin induced paw edema test using a water plethysmometer to observe the antiinflammatory effect of thiamine. Statistical analysis was done by ANOVA, followed by Bonferroni post hoc test. In the interpretation of results, p≤0.05 was considered as significant. Results: In formalin test, thiamine lowered frequency of jerking (after all 3 doses, in both phases) and duration of flexing and licking (200 mg/kg -p<0.001, in late phases; 250 mg/kg (p<0.001), in both phases) significantly. Additionally, thiamine lowered paw edema significantly (p<0.001) in higher 2 doses. Conclusion: This study concludes that, thiamine may have dose dependent antinociceptive and anti-inflammatory effects.

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“…Benfotiamine has also been reported to have anti-inflammatory effects. 76,77 Ang-2 is not expressed by pericytes; pericytes express Ang-1 (and not Ang-2), which promote EC survival signalling, maintenance of endothelial barrier and vascular maturation and quiescence [78][79][80][81] and reduces VEGF-stimulated leukocyte adhesion to ECs. 82 Taurine has been reported to inhibit high glucose-induced apoptosis in retinal pericytes through increased Tie-2 expression and downregulation of Ang-2.…”

Section: Angiopoietin (Ang)-2 Blockagementioning

confidence: 99%

A review of therapies for diabetic macular oedema and rationale for combination therapy

Amoaku

Saker

Stewart

2015

Eye

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Diabetic macular oedema (DMO) is responsible for significant visual impairment in diabetic patients. The primary cause of DMO is fluid leakage resulting from increased vascular permeability through contributory anatomical and biochemical changes. These include endothelial cell (EC) death or dysfunction, pericyte loss or dysfunction, thickened basement membrane, loss or dysfunction of glial cells, and loss/change of EC Glycocalyx. The molecular changes include increased reactive oxygen species, pro-inflammatory changes: advanced glycation end products, intracellular adhesion molecule-1, Complement 5-9 deposition and cytokines, which result in increased paracellular permeability, tight junction disruption, and increased transcellular permeability. Laser photocoagulation has been the mainstay of treatment until recently when pharmacological treatments were introduced. The current treatments for DMO target reducing vascular leak in the macula once it has occurred, they do not attempt to treat the underlying pathology. These pharmacological treatments are aimed at antagonising vascular endothelial growth factor (VEGF) or non-VEGF inflammatory pathways, and include intravitreal injections of anti-VEGFs (ranibizumab, aflibercept or bevacizumab) or steroids (fluocinolone, dexamethasone or triamcinolone) as single therapies. The available evidence suggests that each individual treatment modality in DMO does not result in a completely dry macula in most cases. The ideal treatment for DMO should improve vision and improve morphological changes in the macular (eg, reduce macular oedema) for a significant duration, reduced adverse events, reduced treatment burden and costs, and be well tolerated by patients. This review evaluates the individual treatments available as monotherapies, and discusses the rationale and potential for combination therapy in DMO.

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Anti-inflammatory effects of benfotiamine are mediated through the regulation of the arachidonic acid pathway in macrophages

Cited by 58 publications

References 58 publications

Analgesic Effects of Thiamine in Male Long Evans Rats

Analgesic Effects of Thiamine in Male Long Evans Rats

Antinociceptive and anti-inflammatory effects of thiamine in Long Evans rats

A review of therapies for diabetic macular oedema and rationale for combination therapy

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