Anti-inflammatory effects of Ciwujianoside C3, extracted from the leaves of Acanthopanax henryi (Oliv.) Harms, on LPS-stimulated RAW 264.7 cells

Kang, Dahye; Kang, Ok‐Hwa; Li, Zhi; Mun, Su‐Hyun; Seo, Yun‐Soo; Kong, Ryong; Zhou, Tian; Liu, Xiangqian; Kwon, Dong‐Yeul

doi:10.3892/mmr.2016.5710

Cited by 12 publications

(7 citation statements)

References 41 publications

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“…For example, a hydrophilic compound, morphine-6-glucuronide are considered to be actively transported in the brain by unknown transporter [20]. There might be something transport system for ciwujianoside C3, eleutheroside M and ciwujianoside B. Pharmacological effects of ciwujianoside C3, eleutheroside M and ciwujianoside B were hardly reported except for following two reports using damaged cell conditions; ciwujianoside C3 inhibits proinflammatory cytokine levels in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells [21], ciwujianoside B reduces DNA damage in bone marrow cells exposed to radiation [22]. No studies reported effects of these three compounds on neuronal function in healthy condition.…”

Section: Discussionmentioning

confidence: 99%

Memory Enhancement by Oral Administration of Extract of Eleutherococcus senticosus Leaves and Active Compounds Transferred in the Brain

Yamauchi

Yoshimatsu

et al. 2019

Nutrients

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The pharmacological properties of Eleutherococcus senticosus leaf have not been clarified although it is taken as a food item. In this study, the effects of water extract of Eleutherococcus senticosus leaves on memory function were investigated in normal mice. Oral administration of the extract for 17 days significantly enhanced object recognition memory. Compounds absorbed in blood and the brain after oral administration of the leaf extract were detected by LC-MS/MS analyses. Primarily detected compounds in plasma and the cerebral cortex were ciwujianoside C3, eleutheroside M, ciwujianoside B, and ciwujianoside A1. Pure compounds except for ciwujianoside A1 were administered orally for 17 days to normal mice. Ciwujianoside C3, eleutheroside M, and ciwujianoside B significantly enhanced object recognition memory. These results demonstrated that oral administration of the leaf extract of E. senticosus enhances memory function, and that active ingredients in the extract, such as ciwujianoside C3, eleutheroside M, and ciwujianoside B, were able to penetrate and work in the brain. Those three compounds as well as the leaf extract had dendrite extension activity against primary cultured cortical neurons. The effect might relate to memory enhancement.

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Section: Discussionmentioning

confidence: 99%

Memory Enhancement by Oral Administration of Extract of Eleutherococcus senticosus Leaves and Active Compounds Transferred in the Brain

Yamauchi

Yoshimatsu

et al. 2019

Nutrients

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“…In addition, it has been reported that this plant exhibits diverse pharmacological activities due to the wide variety of chemical constituents. For example, metabolites of the leaves of A. henryi have strong antioxidant and antiacetyl cholinesterase activities [6], and the 80% methanol fraction of root bark and ciwujianoside C3, which was isolated from leaves of this plant, have significant anti-inflammatory effect in lipopolysaccharide- (LPS-) induced RAW264.7 macrophage cells [5, 11]. Moreover, some glycosides from the leaves of A. henryi have antiadipogenic effect, decreasing lipid accumulation through the inhibition of proliferator-activated receptor gamma (PPAR γ ) and CCAAT/enhancer-binding protein alpha (C/EBP α ) in 3T3-L1 cells [12].…”

Section: Introductionmentioning

confidence: 99%

Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi

Kim

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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The phytochemical investigation on the roots of Acanthopanax henryi (Araliaceae) resulted in the discovery of twenty compounds whose chemical structures were elucidated by the analysis of 1D-, 2D-NMR, mass spectrometry data, other physicochemical properties, and a comparison of the spectral data with the literature. They were identified as (-)-sesamin (1), helioxanthin (2), savinin (3), taiwanin C (4), 6-methoxy-7-hydroxycoumarin (5), behenic acid (6), 3-O-caffeoyl-quinic acid (7), 5-O-caffeoyl-quinic acid (8), 1,3-di-O-caffeoyl-quinic acid (9), 1,4-di-O-caffeoyl-quinic acid (10), 1,5-di-O-caffeoyl-quinic acid (11), (+)-threo-(7R,8R)-guaiacylglycerol-β-coniferyl aldehyde ether (12), (+)-erythro-(7S,8R)-guaiacylglycerol-β-coniferyl aldehyde ether (13), ferulic acid (14), caffeic acid (15), stigmasterol (16), β-sitosterol (17), adenosine (18), syringin (19), and trans-coniferin (20). Among these isolates, compound 3 showed inhibitory activity against lipopolysaccharide- (LPS-) induced nitric oxide (NO) and prostaglandin E2 (PGE2) production with IC50 values of 2.22 ± 0.11 and 2.28 ± 0.23 μM, respectively. The effects of compound 3 were associated with the suppression of LPS-induced expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein. Furthermore, compound 3 negatively regulated the production of interleukin- (IL-) 1β and tumor-necrosis factor- (TNF-) α at the transcriptional level in LPS-stimulated BV2 microglial cells. These antineuroinflammatory effects of compound 3 were mediated by p38 mitogen-activated protein kinase (MAPK).

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“…Moreover, HMGB1 is an important ligand of TLR4 that induces the release of proinflammatory cytokines (55,56). HMGB1-TLR4 signaling induces the activation of MAPK and NF-κB (57), and the activation of NF-κB leads to the increased release of various proinflammatory cytokines, including IL-1β, IL-6 and TNF-α (58–60). The release of proinflammatory cytokines can lead to the recruitment of inflammatory cells into the pulmonary microvasculature (61,62).…”

Section: Discussionmentioning

confidence: 99%

Proinflammatory Effects of Respiratory Syncytial Virus–Induced Epithelial HMGB1 on Human Innate Immune Cell Activation

Rayavara

Kurosky

Stafford

et al. 2018

The Journal of Immunology

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High mobility group box 1 (HMGB1) is a multifunctional nuclear protein that translocates to the cytoplasm, and is subsequently released to the extracellular space during infection and injury. Once released it acts as a damage-associated molecular pattern and regulates immune and inflammatory responses. Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory tract infections in infants and elderly, for which no effective treatment or vaccine is currently available. This study investigated the effects of HMGB1 on cytokine secretion, as well as the involvement of NF-κB and TLR4 pathways in RSV-induced HMGB1 release in human airway epithelial cells (AECs) and its proinflammatory effects on several human primary immune cells. Purified HMGB1 was incubated with AECs (A549 and small alveolar epithelial cells) and various immune cells, and measured the release of proinflammatory mediators, and the activation of NF-κB and p38 MAPK. HMGB1 treatment significantly increased the phosphorylation of NF-κB and p38 MAPK but did not induce the release of cytokines/chemokines from AECs. However, addition of HMGB1 to immune cells did significantly induce the release of cytokines/chemokines and activated the NF-κB and p38 MAPK pathways. We found that activation of NF-κB accounted for RSV-induced HMGB1 secretion in AECs in a TLR4-dependent manner. These results indicated that HMGB1 secreted from AECs can facilitate the secretion of proinflammatory mediators from immune cells in a paracrine mechanism, thus promoting the inflammatory response that contributes to RSV pathogenesis. Therefore, blocking the proinflammatory function of HMGB1 may be an effective approach for developing novel therapeutics.

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Anti-inflammatory effects of Ciwujianoside C3, extracted from the leaves of Acanthopanax henryi (Oliv.) Harms, on LPS-stimulated RAW 264.7 cells

Cited by 12 publications

References 41 publications

Memory Enhancement by Oral Administration of Extract of Eleutherococcus senticosus Leaves and Active Compounds Transferred in the Brain

Memory Enhancement by Oral Administration of Extract of Eleutherococcus senticosus Leaves and Active Compounds Transferred in the Brain

Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi

Proinflammatory Effects of Respiratory Syncytial Virus–Induced Epithelial HMGB1 on Human Innate Immune Cell Activation

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