Anti-Inflammatory Effects of Conditioned Medium of Periodontal Ligament-Derived Stem Cells on Chondrocytes, Synoviocytes, and Meniscus Cells

Huang, Chun Yuh; Vesvoranan, Oraya; Yin, Xue; Montoya, Amanda Kay; Londono, Valeria; Sawatari, Yoh; Garcı́a-Godoy, Franklin

doi:10.1089/scd.2021.0010

Cited by 19 publications

(13 citation statements)

References 70 publications

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“…Some cytokines that have not been detected previously, including morphogenetic protein 2 (BMP2), parathyroid hormone‐related protein (PTHrP), fibroblast growth factors 2 (FGF2), platelet‐derived growth factors (PDGF‐BB), and stromal cell‐derived factor 1 (SDF1), were still not detected in this study. However, some proteins with anti‐inflammatory and immunomodulatory properties, that is, MFG‐E8, PTX3, and THBS1, [ 38,39 ] were detected in both CM groups. Nevertheless, the protein compositions for the two groups are different.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Anti‐inflammatory Capacity of the Conditioned Medium Derived from Periodontal Ligament Stem Cells Modified with an Iron‐Based Nanodrug

Wang,

Sun,

Qin

et al. 2023

Advanced Biology

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Cell‐free therapy using conditioned medium (CM) from mesenchymal stem cells takes full advantage of the bioactive factors secreted by the cells while avoiding disadvantages such as immune rejection and tumor formation due to cell transplantation. In this study, human periodontal ligament stem cells (PDLSCs) are modified with the superparamagnetic iron oxide nanoparticle (SPION)‐based nanodrug ferumoxytol (PDLSC‐SPION). Compared with PDLSCs, PDLSC‐SPION showed good cell viability and better osteogenic differentiation ability. Cell‐free CM is collected and the anti‐inflammatory capacity of PDLSC CM and PDLSC‐SPION CM is assessed by treatment of lipopolysaccharide‐stimulated macrophages and IL‐17‐stimulated human gingival fibroblasts. Both CMs inhibited the expression of proinflammatory cytokines in cells, and the therapeutic effect is more distinct for PDLSC‐SPION CM than PDLSC CM, which may be due to their different proteomic compositions. Therefore, modification of PDLSCs with ferumoxytol enhances the anti‐inflammatory capacity of its CM, making it more potentially useful for the treatment of inflammatory diseases such as periodontitis.

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Section: Discussionmentioning

confidence: 99%

Enhanced Anti‐inflammatory Capacity of the Conditioned Medium Derived from Periodontal Ligament Stem Cells Modified with an Iron‐Based Nanodrug

Wang,

Sun,

Qin

et al. 2023

Advanced Biology

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“…Secretome has been shown to contain transcriptional regulatory factors that drive bone remodeling and growth factors that facilitate osteoblastic differentiation and bone regeneration [ 37 ]. Furthermore, studies have shown that MSC/secretome can significantly reduce the expression of inflammation-related agents in the course of OA [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Attenuation of osteoarthritis progression through intra-articular injection of a combination of synovial membrane-derived MSCs (SMMSCs), platelet-rich plasma (PRP) and conditioned medium (secretome)

et al. 2022

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Purpose Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is the most common joint disease characterized by reduction of joint cartilage thickness, demolition of cartilage surface and new bone formation. To overcome these problems, the purpose of the current research was to evaluate and compare the in vivo effects of synovial membrane-derived mesenchymal stem cell (SMMSCs), platelet-rich plasma (PRP) and conditioned medium (secretome) on collagenase II-induced rat knee osteoarthritis (KOA) remedy. Methods For the first step, SMMSCs were isolated and characterized. Also, secretome was collected from SMMSCs culture. Furthermore, PRP was collect from the rat heart venous blood. Second, two injection of collagenase II with an interval of 3 days was performed in the knee intra-articular space to induce osteoarthritis. Two weeks later, animals were randomly divided into 6 groups. Control group without treatment, positive group: taken an intra-articular sodium hyaluronate injection (0.1 ml), treatment groups taken an intra-articular injection of; treatment 1: SMMSCs (5 × 106), treatment 2: SMMSCs (5 × 106)/secretome (50 µl), treatment 3: SMMSCs (5 × 106)/PRP (50 µl), and treatment 4: SMMSCs (5 × 106)/ secretome (50 µl)/ PRP (50 µl). Three months later, rats were killed and the following assessments were executed: radiography, histopathology, and immunohistochemistry. Results Our findings represented that a combination of the SMMSCs/secretome/PRP had a considerable effect on glycosaminoglycans (GAGs) and collagen II contents, articular cartilage preservation, compared with other groups. In addition, combination of the SMMSCs with PRP and secretome showed the lowest expression of mmp3, while SOX9 had the highest expression in comparison with other groups. Also, SMMSCs-injected groups demonstrated better results compared with positive and control groups. Conclusions Injecting a combination of the SMMSCs/secretome/PRP resulted in better efficacy in terms of joint space width, articular cartilage surface continuity and integrity, sub-chondral bone and ECM constituents such as collagen II. Indeed, transplantation of this combination could be considered as a preliminary therapy for clinical trial study in the future.

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“…Exosomes derived from UC-MSCs, which contained a high level of LncRNA H19, promoted chondrocyte proliferation and inhibited apoptosis in vitro; the exosomes also improved macroscopic assessment and relieved pain levels in a rat model of cartilage defect [42]. Furthermore, exosomes extracted from the conditioned medium of PDLSCs showed anti-inflammatory effects on chondrocytes, synoviocytes, and meniscus cells, mediating the inflammatory processes in various tissues in the joint [63]. AFSC-derived exosomes were found to increase pain tolerance and induce the restoration of hyaline cartilage with good surface regularity in an MIA-induced OA model [46].…”

Section: Exosomes Derived From Different Types Of Mscsmentioning

confidence: 96%

“…In addition to BMSC, embryonic stem cell-derived MSCs (ESC-MSCs) [40], synovial MSCs (SMSC) [37,38], adipose-derived MSCs (ADSC) [21,62], umbilical cord mesenchymal stem cells (UC-MSCs) [42], periodontal ligament-derived stem cells (PDLSCs) [63], amniotic fluid stem cells (AFSCs) [46], and IPFP-MSCs [44,64,65] are other important origins of MSC-derived exosomes in OA treatment [38]. IA injection of ESC-MSC-derived exosomes facilitated the repair of osteochondral defects, maintained the chondrocyte phenotype, promoted cartilage formation, reduced matrix degradation, and impeded cartilage destruction both in vitro and in the destabilization of medial meniscus (DMM)-induced OA model in mice [40,66].…”

Section: Exosomes Derived From Different Types Of Mscsmentioning

confidence: 99%

Exosomes in the Pathogenesis, Progression, and Treatment of Osteoarthritis

Fan

2022

Bioengineering

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Osteoarthritis (OA) is a prevalent and debilitating age-related joint disease characterized by articular cartilage degeneration, synovial membrane inflammation, osteophyte formation, as well as subchondral bone sclerosis. OA drugs at present are mainly palliative and do not halt or reverse disease progression. Currently, no disease-modifying OA drugs (DMOADs) are available and total joint arthroplasty remains a last resort. Therefore, there is an urgent need for the development of efficacious treatments for OA management. Among all novel pharmaco-therapeutical options, exosome-based therapeutic strategies are highly promising. Exosome cargoes, which include proteins, lipids, cytokines, and various RNA subtypes, are potentially capable of regulating intercellular communications and gene expression in target cells and tissues involved in OA development. With extensive research in recent years, exosomes in OA studies are no longer limited to classic, mesenchymal stem cell (MSC)-derived vesicles. New origins, structures, and functions of exosomes are constantly being discovered and investigated. This review systematically summarizes the non-classic origins, biosynthesis, and extraction of exosomes, describes modification and delivery techniques, explores their role in OA pathogenesis and progression, and discusses their therapeutic potential and hurdles to overcome in OA treatment.

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Anti-Inflammatory Effects of Conditioned Medium of Periodontal Ligament-Derived Stem Cells on Chondrocytes, Synoviocytes, and Meniscus Cells

Cited by 19 publications

References 70 publications

Enhanced Anti‐inflammatory Capacity of the Conditioned Medium Derived from Periodontal Ligament Stem Cells Modified with an Iron‐Based Nanodrug

Enhanced Anti‐inflammatory Capacity of the Conditioned Medium Derived from Periodontal Ligament Stem Cells Modified with an Iron‐Based Nanodrug

Attenuation of osteoarthritis progression through intra-articular injection of a combination of synovial membrane-derived MSCs (SMMSCs), platelet-rich plasma (PRP) and conditioned medium (secretome)

Exosomes in the Pathogenesis, Progression, and Treatment of Osteoarthritis

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