Anti-inflammatory effects of conjugated linoleic acid isomers and essential fatty acids in bovine mammary epithelial cells

Dipasquale, D.; Basiricò, Loredana; Morera, P.; Primi, Riccardo; Tröscher, A.; Bernabucci, U.

doi:10.1017/s1751731117003676

Cited by 44 publications

(38 citation statements)

References 32 publications

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“…In addition, other studies have clearly marked CLA as a proinflammatory inductor [56,57], indicating that this effect could be greatly heterogeneous depending on physiological conditions, animal species and the dose of CLA. In addition, initial studies in cells showed that CLA, specifically t10,c12, presented ameliorating effects over the production of reactive oxygen species (ROS), correlating to lower gene expression of inflammatory markers [58]. Some studies in humans revealed consistent improvements in inflammation with a reduction in TNF-α (tumour necrosis factor α) and IL-1β (interleukin 1-β) levels [59], while others found increases in TNF-α values [56].…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effects of a Low-dose of Conjugated Linoleic Acid on Body Weight Gain and other Cardiometabolic Risk Factors in Cafeteria Diet-fed Rats

et al. 2020

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Conjugated linoleic acid (CLA) is a dietary supplement that has been shown to improve obesity. However, some authors have associated high doses of CLA supplementation with liver impairment and insulin resistance. The aim of this study was to assess whether the consumption of low doses of CLA maintained the beneficial effects on the main metabolic disturbances associated with metabolic syndrome (MetS) but prevented the occurrence of non-desirable outcomes associated with its consumption. Male Wistar rats, fed standard or cafeteria (CAF) diet for 12 weeks, were supplemented with three different low doses of CLA in the last three weeks. Both biochemical and H1 NMR-based metabolomics profiles were analysed in serum and liver. The consumption of 100 mg/kg CLA, but not doses of 200 and 300 mg/kg, ameliorated the increase in body weight gain as well as the serum concentrations of glucose, insulin, cholesterol, triglyceride, diglyceride, and total phospholipid induced by a CAF diet. In turn, CLA reverted the increase in lactate, alanine, and glucose concentrations in the liver of these animals, but enhanced hepatic cholesterol accumulation without any detrimental effect on liver function. In conclusion, a low dose of CLA corrected the adverse effects associated with MetS without compromising other metabolic parameters.

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Section: Discussionmentioning

confidence: 99%

Beneficial Effects of a Low-dose of Conjugated Linoleic Acid on Body Weight Gain and other Cardiometabolic Risk Factors in Cafeteria Diet-fed Rats

et al. 2020

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“…in the present study, cells in the hypoxia group showed increased excretion of conjugated linoleic acid; this is a polyunsaturated fatty acid that has been reported to stimulate the angiogenesis of placental trophoblast cells in the first trimester (56). In addition, conjugated linoleic acid suppresses roS production via the upregulation of peroxisome proliferator-activated receptor γ expression (57,58).…”

Section: Discussionmentioning

confidence: 49%

Metabolic disparities of different oxidative stress‑inducing conditions in HTR8/SVneo cells

et al. 2019

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Placental oxidative stress is present throughout the duration of pregnancy, but it is when oxidative stress exceeds the normal physiological level that complications can occur. Trophoblast cell lines are commonly utilized for oxidative stress research due to their distinct uniform cell population and easy-to-apply interventions. However, conflicting results are often reported when different oxidative stress cell models are used. in this study, the aim was to characterize the intracellular and extracellular metabolite profiles of different oxidative stress cell models commonly used in the research of pregnancy complications. HTr8/SVneo human trophoblast cell lines were treated with five different oxidative stress-inducing conditions: Hypoxia (1% oxygen); hypoxia and reoxygenation; cobalt chloride (cocl 2 ; 300 µmol/l); sodium nitroprusside (SnP; 2.5 mmol/l); and the serum of women with preeclampsia (10% v/v). intracellular metabolites were extracted from cells and extracellular metabolites were collected from spent media for metabolomic analysis via gas chromatography-mass spectrometry. The results demonstrated that there were distinct differences in the intracellular and extracellular metabolome between the different cell models. Meanwhile, treatments with exogenous drugs, such as cocl 2 and SNP, resulted in more similar metabolite profiles. These disparities between the different oxidative stress cell models will have implications for the applications of these results, and highlight the need for the standardization of oxidative stress cell models in obstetric research. reproduction and development of chinese Ministry of education, chongqing Medical university; 4 Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases, The First Affiliated Hospital of chongqing Medical university, chongqing 400016, P.r. china; 5 liggins institute, university of auckland, auckland 1023, new Zealand; 6 college of Medicine, Biological Sciences and Psychology, university of leicester, leicester, le1 9Hn, uK

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“…Culture medium and supplements were purchased from Sigma-Aldrich (Milano, Italy). For this study, BME-UV1 cells at passage number between 39 and 41 were used (Dipasquale et al, 2018). The dissociation of the cell monolayer and subcultures was carried out every 2 or 3 days.…”

Section: Cell Culturementioning

confidence: 99%

“…Reactive oxygen species were also determined in cells. The concentration 50 μM of H 2 O 2 and 20 μM of LPS were selected on a previous preliminary cytotoxicity study and on results from other published studies (Basiricò et al, 2017;Dipasquale et al, 2018;Mastrogiovanni et al, 2018).…”

Section: Experimental Designmentioning

confidence: 99%

“…The gene expression of bovine pro (tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6) and anti-inflammatory IL-10, cytokines that may play important roles in the mammary inflammatory response, were assayed in BME-UV1 cultured under the conditions described above and were carried out by real-time-PCR. All primers and probes sequences used, were previously reported by Dipasquale et al (2018).…”

Section: Rna Isolation and Real-time Pcrmentioning

confidence: 99%

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(-)-Epigallocatechin-3-gallate and hydroxytyrosol improved antioxidative and anti-inflammatory responses in bovine mammary epithelial cells

Basiricò

Morera

Dipasquale

et al. 2019

Animal

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(-)-Epigallocatechin-3-gallate (EGCG), the major phenolic compound of green tea, and hydroxytyrosol (HTyr), a phenol found in olive oil, have received attention due to their wide-ranging health benefits. To date, there are no studies that report their effect in bovine mammary gland. Therefore, the aim of this study was to evaluate the anti-oxidative and anti-inflammatory effects of EGCG and HTyr in bovine mammary epithelial cell line (BME-UV1) and to compare their antioxidant and anti-inflammatory in vitro efficacy. Sample of EGCG was obtained from a commercially available green tea extract while pure HTyr was synthetized in our laboratories. The mammary oxidative stress and inflammatory responses were assessed by measuring the oxidative stress biomarkers and the gene expression of inflammatory cytokines. To evaluate the cellular antioxidant response, glutathione (GSH/GSSH), γ-glutamylcysteine ligase activity, reactive oxygen species and malondialdehyde (MDA) production were measured after 48-h incubation of 50 µM EGCG or 50 µM of HTyr. Reactive oxygen species production after 3 h of hydrogen peroxide (50 µM H2O2) or lipopolysaccharide (20 µM LPS) exposure was quantified to evaluate and to compare the potential protection of EGCG and HTyr against H2O2-induced oxidative stress and LPS-induced inflammation. The anti-inflammatory activity of EGCG and HTyr was investigated by the evaluation of pro and anti-inflammatory interleukins (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-10) messenger RNA abundance after treatment of cells for 3 h with 20 µM of LPS. Data were analyzed by one-way ANOVA. (-)-Epigallocatechin-3-gallate or HTyr treatments induced higher concentrations of intracellular GSH compared to control cells, matched by an increase of γ-glutamylcysteine ligase activity mainly in cells treated with HTyr. Interestingly, EGCG and HTyr prevented oxidative lipid damage in the BME-UV1 cells by a reduction of intracellular MDA levels. (-)-Epigallocatechin-3-gallate and HTyr were able to enhance cell resistance against H2O2-induced oxidative stress. It was found that EGCG and HTyr elicited a reduction of the three inflammatory cytokines TNF-α, IL-1β, IL-6 and an increase of the anti-inflammatory cytokine IL-10. Hydroxytyrosol has proved to be a strong antioxidant compound, and EGCG has shown mainly an anti-inflammatory profile. These results indicated that EGCG and HTyr may provide dual protection because they were able to attenuate oxidative stress and inflammatory responses, suggesting that these phenolic compounds are potential natural alternatives to be used in dairy cattle as feed supplement for reducing the development of oxidative and inflammatory processes related to parturition or as topical treatments for the control of bovine intramammary inflammation.

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Anti-inflammatory effects of conjugated linoleic acid isomers and essential fatty acids in bovine mammary epithelial cells

Cited by 44 publications

References 32 publications

Beneficial Effects of a Low-dose of Conjugated Linoleic Acid on Body Weight Gain and other Cardiometabolic Risk Factors in Cafeteria Diet-fed Rats

Beneficial Effects of a Low-dose of Conjugated Linoleic Acid on Body Weight Gain and other Cardiometabolic Risk Factors in Cafeteria Diet-fed Rats

Metabolic disparities of different oxidative stress‑inducing conditions in HTR8/SVneo cells

(-)-Epigallocatechin-3-gallate and hydroxytyrosol improved antioxidative and anti-inflammatory responses in bovine mammary epithelial cells

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