Anti-Inflammatory Effects of Licochalcone A on IL-1β-Stimulated Human Osteoarthritis Chondrocytes

Jia, Ting; Jian, Qiao; Guan, Di; Chen, Tianxin

doi:10.1007/s10753-017-0630-5

Cited by 71 publications

(52 citation statements)

References 33 publications

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“…As the component of HSDTT, Atractylodes lancea (Thumb. )DC not only reduce the expression of in ammatory cytokines in serum, but also decreases the expression of beclin-1 protein in synovial tissue of rat [28].Moreover, Radix Angelicae pubescentis and licorice all have a great effect on alliviating in ammtory ,and Radix Angelicae pubescentis has been shown to relieve pain by inhibiting the expression of TNF-α and IL-1β [29,30]. Angelicae Sinensis Radix inhibits apoptosis of chondrocyte via suppressing JNK and p38MAPK pathways [31].…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology Approach and Experimental Verification of Huashi Dingtong Decoction Against Knee Osteoarthritis

Haiya

Lai-Jun

Zhang

et al. 2020

Preprint

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Background: The aim of this study is to clarify the ingredients and targets of HSDTT against KOA by network pharmacology,and to verify the mechanism of HSDTT in treatment of KOA in vivo.Methods: Ingredient-target network for HSDTT and KOA was created to identify the potential targets, protein-protein interaction network was used to find the key targets of HSDTT in treatment for KOA, GO enrichment and KEGG pathway was conducted to illuminate the pathway related to KOA treat by HSDTT. Rat model of KOA was established by joint injection in papain.The morphology of cartilage were assessed by H&E. ELISA was used to detect the contents of inflammation cytokines in synovial fluid and synovium. The expression of the pathway protein were assessed by PCR.Results: The results of network pharmacology demonstrate that there are 440 ingredients of HSDTT against knee osteoarthritis by 478 targets.The KEGG enrichment analysis showed that PI3K-Akt signaling pathway, MAPK signaling pathway were the key pathways for HSDTT to treat KOA. Morphology of cartilage was improved in the HSDTT group when compared with the model group. Our experiment show that HSDTT can reduced the expressions of p38 and p53 in cartilage, increased the expression of collagenⅡ. The contents of IL-1β and TNF-α in the synovium and COX-2 and PGE-2 in synovial fluid were decreased significantly in the HSDTT group when compared with the model group.Conclusions: Our study indicadites that HSDTT is capable to alleviate inflammation and delay the progression of KOA by p38MAPK signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Network Pharmacology Approach and Experimental Verification of Huashi Dingtong Decoction Against Knee Osteoarthritis

Haiya

Lai-Jun

Zhang

et al. 2020

Preprint

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“…Furthermore, although studies associated with the biological effects of Lico-E have reported lower efficacy than that of Lico-A, the biological effects of both licochalcones exhibit similar properties. According to recently reported studies associated with Lico-A, various biological effects such as neuroprotective [26], chondroprotective [27,28], antithrombotic [29], antimicrobial [30], and antiviral activities [30] have been reported. In addition, recent studies have also reported reduction in chronic allergic contact dermatitis as well as antidiabetic, anti-inflammatory, and antimicrobial effects.…”

Section: Discussionmentioning

confidence: 99%

Anti-tumor effect of licochalcone-E is mediated by caspase-dependent apoptosis through extrinsic and intrinsic apoptotic signaling pathways in KB cancer cells

et al. 2017

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·············································································································································································This study investigates the anti-cancer effects of licochalcone-E (Lico-E), a phenolic chalconoid derived from the genus Glycyrrhiza, in the human KB squamous cancer cell. Concentration-dependent cytotoxicity in KB cells increased following 24 hours of treatment with 12.5, 25, or 50 µg/ml Lico-E, with an estimated IC 5 0value of approximately 25 µg/ml. Chromatin condensation, a typical apoptotic phenomenon, was observed in KB cells treated with Lico-E. Consistent with this finding, Lico-E increased caspase-3 activity in KB cells. FasL, a death ligand associated with extrinsic apoptotic signaling pathways, was significantly up-regulated by Lico-E treatment. Subsequently, the pro-apoptotic factor caspase-8, a part of the extrinsic apoptotic signaling pathway, was activated in a concentrationdependent manner by Lico-E treatments. Expression of anti-apoptotic factors such as Bcl-2 and Bcl-xL, components of the mitochondriadependent apoptotic signaling pathway, significantly decreased following Lico-E treatment. Conversely, expression of pro-apoptotic factors such as Bax, Bad, Apaf-1, and caspase-9 increased with Lico-E concentrations. Finally, Lico-E activated caspase-3 and Poly (ADP-ribose) polymerase (PARP) to induce cell death. Z-VAD-fmk significantly inhibited cell death through suppression of caspase-3 expression in KB cells treated with Lico-E. Taken together, Lico-E induces KB cell death through death receptor and mitochondriadependent apoptotic signaling pathways.

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“…Licochalcone A reduced LPS-induced NF-κB transactivation by inhibiting phosphorylation of p65 at serine 276 and interaction of p65 with p300 [72]. e compound not only indicated an anti-inflammatory effect on IL-1β-stimulated chondrocytes by activating the Nrf2 signaling pathway [73] but also activated Keap1-Nrf2 signaling to inhibit arthritis by enhancing phosphorylation and expression of p62 at serine 349 [74]. ERK and p38 signaling pathways might play a role in attenuation of allergic airway inflammation by licochalcone A [75].…”

Section: Anti-inflammatory Activitymentioning

confidence: 91%

Natural Chalcones in Chinese Materia Medica: Licorice

Wang

Liang

Zhang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Licorice is an important Chinese materia medica frequently used in clinical practice, which contains more than 20 triterpenoids and 300 flavonoids. Chalcone, one of the major classes of flavonoid, has a variety of biological activities and is widely distributed in nature. To date, about 42 chalcones have been isolated and identified from licorice. These chalcones play a pivotal role when licorice exerts its pharmacological effects. According to the research reports, these compounds have a wide range of biological activities, containing anticancer, anti-inflammatory, antimicrobial, antioxidative, antiviral, antidiabetic, antidepressive, hepatoprotective activities, and so on. This review aims to summarize structures and biological activities of chalcones from licorice. We hope that this work can provide a theoretical basis for the further studies of chalcones from licorice.

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Anti-Inflammatory Effects of Licochalcone A on IL-1β-Stimulated Human Osteoarthritis Chondrocytes

Cited by 71 publications

References 33 publications

Network Pharmacology Approach and Experimental Verification of Huashi Dingtong Decoction Against Knee Osteoarthritis

Network Pharmacology Approach and Experimental Verification of Huashi Dingtong Decoction Against Knee Osteoarthritis

Anti-tumor effect of licochalcone-E is mediated by caspase-dependent apoptosis through extrinsic and intrinsic apoptotic signaling pathways in KB cancer cells

Natural Chalcones in Chinese Materia Medica: Licorice

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