Anti-inflammatory effects of methanolic extract of green algae Caulerpa mexicana in a murine model of ulcerative colitis

Bitencourt, Mariana Angélica Oliveira; Silva, Hylarina; Abílio, Gisely Maria Freire; Miranda, George Emmanuel Cavalcanti de; Moura, Adolpho Marlon Antoniol de; Araújo-Júnior, João Xavier de; Silveira, Éricka Janine Dantas da; Santos, Bárbara V. O.; Souto, Janeusa Trindade de

doi:10.1016/j.bjp.2015.10.001

Cited by 18 publications

(16 citation statements)

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“…On the other hand, the treatment of mice with CLP at the dose of 4 mg/kg led to attenuation of clinical signs of experimental colitis ( Figure 3 A) by delaying the weight loss appearance until the sixth day ( Figure 3 B) and the onset of clinical signs until the fourth day ( Figure 3 C,D). These data are in accordance with previous studies from our group that have shown that the methanolic extract of C. mexicana ameliorates the clinical signs in animals with ulcerative colitis induced by DSS [ 30 ]. Similar to our data, the treatment of animals with the indole alkaloid Sinomenine protected the lungs of the mice against the damage caused by lipopolysaccharide in the model of acute lung injury, and this protection, according to the authors, happened due to an increased adenosine A2A (A2AR) receptor signaling, which has anti-inflammatory activity [ 31 ].…”

Section: Discussionsupporting

confidence: 93%

The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus Caulerpa, Attenuated Colon Damage in Murine Colitis Model

et al. 2018

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Caulerpin (CLP), an alkaloid from algae of the genus Caulerpa, has shown anti-inflammatory activity. Therefore, this study aimed to analyze the effect of CLP in the murine model of peritonitis and ulcerative colitis. Firstly, the mice were submitted to peritonitis to evaluate which dose of CLP (40, 4, or 0.4 mg/kg) could decrease the inflammatory infiltration in the peritoneum. The most effective doses were 40 and 4 mg/kg. Then, C57BL/6 mice were submitted to colitis development with 3% dextran sulfate sodium (DSS) and treated with CLP at doses of 40 and 4 mg/kg. The disease development was analyzed through the disease activity index (DAI); furthermore, colonic tissue samples were submitted to histological analysis, NFκB determination, and in vitro culture for cytokines assay. Therefore, CLP at 4 mg/kg presented the best results, triggering improvement of DAI and attenuating the colon shortening and damage. This dose was able to reduce the TNF-α, IFN-γ, IL-6, IL-17, and NFκB p65 levels, and increased the levels of IL-10 in the colon tissue. Thus, CLP mice treatment at a dose of 4 mg/kg showed promising results in ameliorating the damage observed in the ulcerative colitis.

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Section: Discussionsupporting

confidence: 93%

The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus Caulerpa, Attenuated Colon Damage in Murine Colitis Model

et al. 2018

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“…Also, tubastrine and orthidine F showed an inhibitory effect on neutrophil infiltration in this in vivo model. Ascidiathiazones A (28) and B (29), two thiazone-containing quinolinequinone alkaloids obtained from Aplidium spp., had a similar effect on superoxide production by PMA-stimulated neutrophils in vitro and in an in vivo murine gout model [85]. Kottamide D (30), the imidaloze-containing alkaloid obtained from the ascidian Pycnoclavella kottae, was also able to reduce superoxide synthesis by PMA and N-formylmethionyl-leucyl-phenylalanine (fMLP)-activated neutrophils in vitro [86].…”

Section: Other Invertebrate Animals As Sources Of Marine Alkaloidsmentioning

confidence: 99%

“…Caulerpa mexicana aqueous and methanolic extracts were able to reduce IL-6, IL-12, and TNF-α production by LPS-stimulated macrophages and leukocyte migration in murine zimosan-induced peritonitis and air pouch inflammation models and decreased xylene-induced ear edema [27]. Subsequently, we observed the anti-inflammatory activity of a C. mexicana methanolic extract in a murine model of dextran sulfate sodium (DSS)-induced ulcerative colitis, with the attenuation of the clinical signs of the disease and a significant reduction of IFN-γ, IL-6, IL-12, IL-17A, and TNF-α levels, together with the preservation of the morphological structure of the colon and a reduction of inflammatory tissue infiltrates [28]. In fact, in another study, different extracts of C. mexicana and Caulerpa sertularioides showed anti-inflammatory activity in a murine model of carrageenan-induced peritonitis, reducing leukocyte migration to the lesion site [94].…”

Section: Anti-inflammatory Alkaloids In Marine Algaementioning

confidence: 99%

“…In addition to these compounds, seaweed produce a number of secondary metabolites with remarkable biological activity, such as phenolic compounds, polysaccharides, carotenoids, lectins, steroids, polyketides, and many others [17]. Several studies have shown that seaweed contain a range of bioactive substances with diverse pharmacological potential, such as antiviral [18], antibiotic and antiendotoxic [19], antifungal [20], antiparasitic [21], antioxidant [22], anti-ageing [23], antinociceptive [24], anti-tumor [25], anti-diabetic [26], anti-inflammatory, and immunomodulatory [27,28] effects. Among all, the anti-inflammatory activity of compounds derived from seaweed is one of the largest bioprospecting areas in marine natural products.…”

Section: Introductionmentioning

confidence: 99%

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Marine Alkaloids with Anti-Inflammatory Activity: Current Knowledge and Future Perspectives

2020

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Alkaloids are nitrogenous compounds with various biological activities. Alkaloids with anti-inflammatory activity are commonly found in terrestrial plants, but there are few records of the identification and characterization of the activity of these compounds in marine organisms such as fungi, bacteria, sponges, ascidians, and cnidarians. Seaweed are a source of several already elucidated bioactive compounds, but few studies have described and characterized the activity of seaweed alkaloids with anti-inflammatory properties. In this review, we have gathered the current knowledge about marine alkaloids with anti-inflammatory activity and suggest future perspectives for the study and bioprospecting of these compounds.

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“…As algas têm sido intensivamente estudadas, visto que apresentam potencial para numerosas aplicações práticas (CHRISTENSON; SIMS, 2011). As pesquisas recentes envolvendo esses organismos visam principalmente objetivos comerciais, como a produção de biocombustíveis (GUCCIONE et al, 2014) e de outros produtos de valor agregado, incluindo lipídeos (LIZZUL et al, 2013), esteroides (LANG et al, 2007), pigmentos (MIZOGUCHI et al, 2011 e fármacos (BITENCOURT et al, 2015). Apesar das inúmeras aplicações econômicas, os custos de produção ainda são altos, e em grande parte devido às dificuldades da separação sólido-líquido das microalgas de seus meios de crescimento (DANQUAH et al, 2009).…”

Section: Introductionunclassified

Remoção de microalgas por pré-ozonização e flotação por ar dissolvido

Vieira¹

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Dedico este trabalho aos meus pais, Maria de Fátima e Estácio, pelo amor incondicional e apoio; ao meu irmão Ricardo, por ter sido sempre o melhor que irmão que alguém poderia ter; À Laiane, meu amor e minha companheira. AGRADECIMENTOS À Deus, por me guiar, me auxiliar e me dar forças em momentos difíceis, e pelos presentes que me deu, como os bons momentos, a família, o aprendizado, as conquistas e os amigos. Aos meus pais, Estácio e Maria de Fátima, por me darem amor incondicional, por lutarem por mim e pelo bem de nossa família. À minha avó Celita (In memoriam), que jamais me deixou em dificuldades e nunca desistiu de cuidar dos que ama. A realização do meu Mestrado é dedicada a ela, que sempre me apoiou na busca dos meus sonhos. Ao meu irmão Ricardo, que sempre foi um grande irmão e amigo, parceiro para todas as horas, que é um espelho para lutar pela vida e pelas conquistas. À Universidade de São Paulo pela oportunidade de cursar o mestrado, um motivo de muito orgulho para mim. Ao Professor Marco Antônio Penalva Reali, pelos ensinamentos, pela confiança e pela dedicação em ensinar. Por ter sido um excelente orientador, por me mostrar o que é ser um profissional da engenharia e da educação superior. À CNPq, pela bolsa concedida, e à FAPESP pelos recursos e apoio necessários à implementação do projeto temático sobre recuperação de nutrientes através de microalgas, através do processo FAPESP 2013/50351-4. Ao Professor Luiz Antônio Daniel, sempre me auxiliou em minhas tarefas, me deu conselhos e ensinamentos, foi fundamental para o andamento do meu projeto de pesquisa. Ao Professor Gustavo Ribeiro, pelo apoio no estudo das microalgas e na implementação do projeto, sempre sendo muito acessível e compreensivo. À Ana Paula Sueitt, pela ajuda extraordinária na execução da pesquisa e pelo auxílio na elaboração da dissertação. Ao Alcino, que nos auxiliou em todas as etapas de nosso trabalho com seus conhecimentos técnicos e práticos, realizando obras e consertando problemas. E que, além disso, se mostrou uma grande pessoa e amigo. À minha namorada Laiane, pelo apoio irrestrito, confiança, amor, cumplicidade. Por ter me dado força em todos os momentos, sendo bons ou maus, e por melhorar significativamente os meus dias, tornando-os mais felizes. Aos companheiros e amigos de laboratório, em especial, à Maria Teresa, especialista de laboratório, que me ensinou e me auxiliou em todas as etapas da pesquisa. Ao Thadeu Sahão, que se tornou um grande amigo, e que me auxiliou muito em minha pesquisa. Agradeço ao Gabriel Sacchi, que me ajudou muito, repassando seus conhecimentos sobre algas e por ter me deixado uma ótima estrutura de pesquisa já pronta. Agradeço muito a ajuda do Paulo dos Santos, que foi sempre um bom amigo e me ajudou quando precisei de conselhos sobre a pesquisa com algas e seus procedimentos. A todos os alunos do LATAR e BIOTACE, que proporcionaram muitos momentos de amizade e descontração

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Anti-inflammatory effects of methanolic extract of green algae Caulerpa mexicana in a murine model of ulcerative colitis

Cited by 18 publications

References 27 publications

The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus Caulerpa, Attenuated Colon Damage in Murine Colitis Model

The Bisindole Alkaloid Caulerpin, from Seaweeds of the Genus Caulerpa, Attenuated Colon Damage in Murine Colitis Model

Marine Alkaloids with Anti-Inflammatory Activity: Current Knowledge and Future Perspectives

Remoção de microalgas por pré-ozonização e flotação por ar dissolvido

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