Anti-Inflammatory Effects of Resveratrol and Oligostilbenes from Vitis thunbergii var. taiwaniana against Lipopolysaccharide-Induced Arthritis

Abstract

Vitis thunbergii Sieb. and Zucc. var. taiwaniana Lu is an endemic plant in Taiwan used as a dietary supplement for bone health. In this study, human chondrocytes were induced to produce COX-2, MMP-3, -13, and PGE(2) by LPS. An (18)F-FDG microPET imaging system was used to evaluate the anti-inflammatory arthritic effects in vivo. Six stilbenes, resveratrol (1), (+)-ε-viniferin (2), ampelopsin C (3), ampelopsin A (4), (-)-vitisin B (5), and (+)-vitisin A (6), were isolated from the stem part of V. thunbergii, which displayed the strongest PGE(2) inhibition. Among these compounds, 1 significantly decreased COX-2 activity, PGE(2), MMP-3, and -13 production in vitro, and (18)F-FDG uptake and serum PGE(2) in rabbits in vivo. Anti-inflammatory effects were enhanced through the combined usage of 1 and other oligostilbenes. Taken together, the synergistic effects of 1 and oligostilbenes resulted in stem part extracts with lower 1 content displaying the better anti-inflammatory arthritis effects.

Anti-Inflammatory Effects of Vitis thunbergii var. taiwaniana on Knee Damage Associated with Arthritis

“…First, the microPET imaging system was helpful in measuring acute inflammatory arthritis in our previous study. 4 SUV values calculated by the ASIPro VM TM analysis software were used to quantify the parameter in the micro-PET image. As shown in Figure 3, the VT-SR obviously decreased the LPS-induced inflammatory arthritic status in the microPET analysis and lowered the serum PGE 2 concentration.…”

“…4 PHCs were incubated at 37°C in a humidified atmosphere of 5% CO 2 using DMEM and confirmed by a Safranin O solution (data not shown). Experiments were performed with cells of about the third passage.…”

“…The assay protocol and data calculation of standard uptake value (SUV), the most popular quantitation parameter of PET, were modified from those of our previous study. 4 We used a Concorde microPET R4 scanner (Concorde Microsystems, Knoxville, TN, USA) for the PET analysis. Rabbits received an average of 38.0 MBq of FDG by an intravenous bolus injection into one of the ear veins.…”

“…3 Quercetin and resveratrol significantly alleviated inflammatory responses and inflammation-related arthritis. 4,5 Vitis thunbergii Sieb. et Zucc.…”

“…In addition, resveratrol in VT had synergistic PGE 2 -inhibitory effects with other oligostilbenes, such as (+)-e-viniferin, in LPS-induced primary human chondrocytes. 4 Hence, the goal of this study was to determine the anti-inflammatory knee damage-associated anti-arthritic effects of VT. Different fractions of the VT water extract were screened for antioxidative and antiinflammatory effects in vitro.…”