Anti-Inflammatory Effects of Thyme Essential Oil in Mice

Juhás, Štefan; Bujňáková, Dobroslava; Rehák, P; Číkoš, Štefan; Czikková, S.; Veselá, Jarmila; Il'ková, Gabriela; Koppel, Juraj

doi:10.2754/avb200877030327

Cited by 41 publications

(25 citation statements)

References 33 publications

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“…In the present study, acute inflammation was produced by an injection of carrageenan in the right hind paw of the mice [52]. During injection, inflammatory response produced increase in vascular permeability and cellular infiltration leading to edema formation (an increase of paw volume), as a result of extravasation response in mice.…”

Section: Discussionmentioning

confidence: 88%

Anti-inflammatory and analgesic activities of solvent fractions of the leaves of Moringa stenopetala Bak. (Moringaceae) in mice models

Tamrat

Nedi

Assefa

et al. 2017

BMC Complement Altern Med

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BackgroundMany people still experience pain and inflammation regardless of the available drugs for treatments. In addition, the available drugs have many side effects, which necessitated a quest for new drugs from several sources in which medicinal plants are the major one. This study evaluated the analgesic and anti- inflammatory activity of the solvent fractions of Moringa stenopetala in rodent models of pain and inflammation.MethodsSuccessive soxhlet and maceration were used as methods of extractions using solvents of increasing polarity; chloroform, methanol and water. Swiss albino mice models were used in radiant tail flick latency, acetic acid induced writhing and carrageenan induced paw edema to assess the analgesic and anti-inflammatory activities. The test groups received different doses (100 mg/kg, 200 mg/kg and 400 mg/kg) of the three fractions (chloroform, methanol and aqueous). The positive control groups received morphine (20 mg/kg) or aspirin (100 mg/kg or 150 mg/kg) based on the respective models. The negative control groups received the 10 ml/kg of vehicles (distilled water or 2% Tween 80).ResultsIn all models, the chloroform fraction had protections only at a dose of 400 mg/kg. However, the methanol and aqueous fraction at all doses have shown significant central and peripheral analgesic activities with a comparable result to the standards. The aqueous and methanol fractions significantly reduced carrageenan induced inflammation in a dose dependent manner, in which the highest reduction of inflammation was observed in aqueous fraction at 400 mg/kg.ConclusionThis study provided evidence on the traditionally claimed uses of the plant in pain and inflammatory diseases, and Moringa stenopetala could be potential source for development of new analgesic and anti-inflammatory drugs.

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Section: Discussionmentioning

confidence: 88%

Anti-inflammatory and analgesic activities of solvent fractions of the leaves of Moringa stenopetala Bak. (Moringaceae) in mice models

Tamrat

Nedi

Assefa

et al. 2017

BMC Complement Altern Med

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“…The dietary effect of oregano oil has already been studied in vitro (Burt 2004) and in vivo (Botsoglou et al 2002;Garcia et al 2007) conditions. Numerous works have been devoted to the antioxidant effect (Botsoglou et al 2002), anti-inflammatory activity (Juhás et al 2008), antibacterial properties (Burt et al 2005), and anticoccidial activities (Giannenas et al 2003). Mechanism for the interaction of plants including Oregano spp.…”

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confidence: 99%

Intestinal mucin dynamic and leukocytic responses of chickens infected with Eimeria acervulina and fed oregano supplemented diet

Major¹,

Révajová

Levkut

et al. 2011

Acta Vet. Brno

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We studied the effect of oregano supplemented diet on mucin dynamics in small intestine, peripheral blood leukocytes, and jejunal immunocompetent cells in ROSS 308 hybrid broilers infected with Eimeria acervulina chickens' oocyst. From the day of hatching chicks of groups 1 and 2 were fed a commercial diet without anticoccidial drug, the diet of group 3 was supplemented with oregano (0.707g·kg -1 ), and that of group 4 with anticoccidial drug (Robenidin hydrochloride -33 mg·kg -1 ). Chickens of groups 2, 3, and 4 were inoculated with E. acervulina oocysts (25.10 3 ) on day 12. The samples were collected on 3, 10, 17 days post infection (dpi). In blood on 3 dpi significant increase of leukocytes was found in group 3 compared to groups 1, 2, and 4, higher density of IgM+ cells in group 3 than group 2, and on 10 dpi phagocytic activity of group 3 was higher than group 1. Number of jejunal CD4+ and CD8+ cells in group 3 was consistent with values in group 4, despite higher density of E. acervulina meronts on 10 dpi. The quantity of jejunal mucin adherent layer of group 3 was similar to that in group 4. Counts of oocysts in faeces were lower in group 3 than group 2. Results suggest that dietary supplementation of oregano to chickens infected with E. acervulina has a modulating effect on some blood indicators and functions of phagocytes. The beneficial effect of oregano components on jejunal mucin quantity and its turnover is the first finding published in relation to oregano and coccidia.

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“…Carrageenan-induced mouse paw oedema has been widely used for analysis of the antiinflammatory effects of plant extracts and essential oils (Maruyama et al 2006;Fernandes et al 2007;Juhás et al 2008a). Experimental colitis in mice and rats induced by the administration of various chemical agents is frequently used to study various compounds as possible therapeutic agents for inflammatory bowel disease (Ikeda et al 2008).…”

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confidence: 99%

“…These alternative treatments include herbal therapy consisting of anti-oxidant, anti-inflammatory and antimicrobial plants administration, too. We have described very recently that thyme essential oil in the diet was able to attenuate murine TNBS-induced colitis depending on the concentration used (Juhás et al 2008a).…”

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confidence: 99%

Anti-Inflammatory Effects of Rosmarinus officinalis Essential Oil in Mice

et al. 2009

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Essential oils are plant secondary metabolites possessing various pharmacological properties, primarily anti-oxidative, antimicrobial or immunomodulative, but they can exhibit toxic and allergic effects as well. The aim of this study was to compare the effects of Rosmarinus officinalis essential oil dietary administration in carrageenan paw oedema and trinitrobenzene sulfonic acid (TNBS) colitis. ICR mice received rosemary essential oil at three concentrations (1 250, 2 500 and 5 000 ppm) in the standard laboratory diet starting two weeks before the experiments. The inflammation of paws induced by carrageenan application was evaluated by measurement of paw swelling, paw weight and myeloperoxidase activity. In the TNBS model the mice were killed by cervical dislocation 3 days after colitis induction and the mortality, changes in the body weight of mice, colon weight : body weight ratio, macroscopical scores and myeloperoxidase activity were analysed. Furthermore, IL-1β and IL-6 cytokine levels in colonic tissue were quantified using ELISA assay. Dietary supplementation with 5 000 ppm of rosemary essential oil initially after 2 h increased but after 24 h suppressed the extent of paw oedema. The same dose in the TNBS model exhibited protective effects on colonic mucosa and significantly decreased macroscopic scores for colonic inflammation. On the other hand, in colon samples from mice fed the diet with 1 250 ppm of rosemary essential oil we detected decreased myeloperoxidase activity and significantly lower levels of IL-6 compared to TNBS control animals.Our results indicate that rosemary essential oil is able to influence several variables of murine experimental inflammatory models depending on the concentration used. We conclude that the anti-inflammatory effects of rosemary essential oil should be interpreted carefully due to its timeand dose-related effects.

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Anti-Inflammatory Effects of Thyme Essential Oil in Mice

Cited by 41 publications

References 33 publications

Anti-inflammatory and analgesic activities of solvent fractions of the leaves of Moringa stenopetala Bak. (Moringaceae) in mice models

Anti-inflammatory and analgesic activities of solvent fractions of the leaves of Moringa stenopetala Bak. (Moringaceae) in mice models

Intestinal mucin dynamic and leukocytic responses of chickens infected with Eimeria acervulina and fed oregano supplemented diet

Anti-Inflammatory Effects of Rosmarinus officinalis Essential Oil in Mice

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