Anti-Inflammatory Effects of Tongbi-san(通痺散) Extract on RAW264.7 Macrophages

Abstract

Objectives : This study is to investigate the anti-inflammatory and anti-oxidant effects of Tongbi-san extract (TS) on RAW264.7 macrophages using by cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin E 2 (PGE 2 ) production and 1,1-diphenyl-2-picryl ghdrazyl (DPPH) free radical scavenging capability.Methods : Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The production of NO was measured by Griess assay. The production of PGE 2 was measured by immunoassay. And the anti-oxidant activity was measured by the DPPH method.Results : TS did not increased significantly compared to the TS untreated group in the cell cytotoxicity. TS inhibited NO and PGE 2 production in lipopolysaccharide-stimulated RAW 264.7 cells. TS had the DPPH free radical scavenging capability. Conclusion : The anti-inflammtory and anti-oxidant effects of TS may be use for a treatment of anti-inflammatory diseases.