Cnidii Rhizoma is a perennial herb belonging to the Umbelliferae family. Its origin is China, and it is distributed in Korea and Japan. It has been reported that phthalide and phenolic compounds are the main ingredients. To confirm changes in metabolites from Gyeongbuk production area and harvested time, metabolite profiling and multivariate statistical analysis techniques were performed. As a result, a total of thirty-two compounds including sixteen of phthalide, phenolic acid, three of coumarin, one of aromatic glycoside, flavonoid, and steroid, two of phenylpropanoid, and unknown compounds were identified. In addition, the s-plot model of Orthogonal Partial Least Squares-Discriminant Analysis was applied to identify chemical markers that contribute to discrimination between groups by production area and harvest time. The clustering tendency of the 2019 and 2020 groups in Gyeongbuk-Bonghwa and Gyeongbuk-Yeongyang areas was confirmed. The chemical markers which were peaks No. 4, 25, and 28 identified were phenolic acid, phenylpropanoid, and phthalide glycoside.
Keywords Cnidii Rhizoma• Metabolites profiling • Multivariate statistical analysis • Phenolic compounds • Phthalide compounds • Ultra-performance liquid chromatography-quadrupole time-offlight mass spectrometry 서 론 대사체학(Metabolomics)은 생물학적 시스템상에서 모든 대사산 물을 종합적으로 분석하는 학문이며, 이는 전사체학(transcriptomics) 및 단백체학(proteomics)을 보완하고 저분자의 분석 물질(<1,800 Da)을 정량적 및 정성적 평가를 제공할 수 있다[1]. 최근 대사체학에는 핵자기 공명 분석(Nuclear Magnetic Resonance), 질량분석기 (Mass spectrometry)와 결합된 기체크로마토그래피 (Gas chromatography) 또는 고성능 액체크로마토그래피(High performance liquid chromatography) 및 초고성능 액체크로마토