Anti-inflammatory efficacy and relevant SAR investigations of novel chiral pyrazolo isoquinoline derivatives: Design, synthesis, in-vitro, in-vivo, and computational studies targeting iNOS

Akhtar, Maryam; Niu, Jiabin; Zhu, Yunlong; Luo, Zhaoyi; Tian, Tao; Dong, Yuanliang; Wang, Yuan; Fareed, Muhammad Subaan; Lin, Li

doi:10.1016/j.ejmech.2023.115412

Cited by 7 publications

(1 citation statement)

References 32 publications

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“…The NF-kB/I-B pathway is triggered by NF-kB, which then enhances iNOS activity. The pathophysiology of intricate inflammatory illnesses is assumed to be significantly influenced by the overexpressed iNOS, which would lead to excessively elevated NO levels [40]. Our findings show that Cup significantly increased the levels of inflammatory mediators like IL-1, iNOS, NF-kB, and IL-17.…”

Section: Discussionmentioning

confidence: 60%

New Insight into the Possible Roles of L-Carnitine in a Rat Model of Multiple Sclerosis

Safwat,

Aboonq,

El Tohamy

et al. 2023

Brain Sciences

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Objective: We investigated the effect of L-carnitine (LC) on cuprizone (Cup) demyelinating rat model and its possible underlying mechanisms. Methods: Thirty male Sprague–Dawley (SD) rats were randomly allocated to three groups: the normal control group; the Cup group, in which Cup was administrated at a dose of 450 mg/kg per day orally via gastric gavage for 5 weeks; and the Cup + LC group, which received the same dose of Cup as the Cup group, except that the rats were treated additionally with LC 100 mg/kg/day orally for 5 weeks. The nerve conduction (NCV) in isolated sciatic nerves was measured; then, the sciatic nerves were isolated for H&E staining and electron microscope examination. The expression of myelin basic protein (MBP), IL-1β, p53, iNOS, and NF-KB by immunohistochemistry was detected in the isolated nerves. A PCR assay was also performed to detect the expression of antioxidant genes Nrf2 and HO-1. In addition, the level of IL-17 was measured by ELISA. Results: There was a significant reduction in NCV in the Cup group compared to normal rats (p < 0.001), which was significantly improved in the LC group (p < 0.001). EM and histopathological examination revealed significant demyelination and deterioration of the sciatic nerve fibers, with significant improvement in the LC group. The level of IL-17 as well as the expression of IL-1β, p53, iNOS, and NF-KB were significantly increased, with significant reduction expression of MBP in the sciatic nerves (p < 0.01), and LC treatment significantly improved the studied parameters (p < 0.01). Conclusion: The current study demonstrates a neuroprotective effect of LC in a Cup-induced demyelinating rat model. This effect might be due to its anti-inflammatory and antioxidant actions.

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Section: Discussionmentioning

confidence: 60%