Anti-inflammatory Mechanism of Rhein in Treating Asthma Based on Network Pharmacology

Abstract

BackgroundNetwork pharmacology was used to study Rhein -target-pathway and to clarify its anti-inflammatory mechanism in the treatment of asthma.and provide a new idea for the treatment of asthmaMethodsThis method, which allows using network pharmacology to figure out the operational mechanism of Rhein-Target-Pathway, defines the effect of anti-inflammatory in treating asthma. The platform of Traditional Chinese Medicine Molecular Mechanism Bioinformatics, a web server for network, is used to get the corresponding target of Rhein and permit molecular docking. Cytoscape3.7.1, a kind of network software, is used to construct Rhein-predicted target network and analyse network topology. Search anti-inflammatory targets in the database of TTD and then construct the PPI network as well as create protein interaction networks that are combined with the Rhein-predicted target network. The anti-inflammatory targets of Rhein should be presented. The asthma genes of human being can be attained from the database of NCBI Gene Database and construct correspondence vivo response network model. Find Anti-inflammatory targets of Rhein against asthma, screen anti-inflammatory targets of Rhein related with Pathogenesis of asthma. Enrichr database is used to analyse signal pathway from anti-inflammatory targets of Rhein KEGG.ResultsAccording to the study, Rhein corresponds to 17 target proteins, four anti-inflammatory targets of Rhein related to asthma(MAPK14, EGFR, ERBB2, TNFRSF1A) are probably the most important targets where asthma is treated by Rhein.ConclusionsThese four anti-inflammatory targets of Rhein related to asthma are probably the key targets in the treatment of asthma by using Rhein. For the purpose of preventing the occurrence as well as development of asthma and delaying the progress of the disease, one or some of the four anti-inflammatory targets of Rhein related to asthma can be controlled.