Anti-inflammatory mechanism of simvastatin in mouse allergic asthma model

Kim, Dae Yong; Ryu, Seunghyong; Lim, Ji Eun; Lee, Yun Song; Ro, Jai Youl

doi:10.1016/j.ejphar.2006.11.027

Cited by 120 publications

(128 citation statements)

References 45 publications

Supporting

Mentioning

118

Contrasting

Order By: Relevance

“…Blood was collected by cardiac puncture and analyzed for OVA-specific IgE by ELISA [31]. Briefly, microplates coated with rat anti-mouse IgE were treated with mouse sera followed by biotinylated OVA.…”

Section: Methodsmentioning

confidence: 99%

“…BAL fluid was collected, stained and counted using methods previously described [31]. Numbers of infiltrated inflammatory cells or mast cells stained with Diff-Quick and May Grünwald-Giemsa [32], respectively, were quantified under microscopy (see additional file for details).…”

Section: Methodsmentioning

confidence: 99%

“…After BAL had been performed, lungs tissue sections embedded in paraffin (3 μm) were stained with hematoxylin and eosin (H & E) for general morphology, with periodic acid-Schiff (PAS) for identification of goblet cells [31], with van Gieson for collagen deposition [33] and with May Grünwald-Giemsa for mast cells [32] (see additional file for details). Collagen amounts were assessed by the Sircol collagen assay according to the manufacturer's instruction (see additional file for details) [34].…”

Section: Methodsmentioning

confidence: 99%

“…Immnunoblotting was conducted using a method previously described [31]. Briefly, BAL cells (1 × 10 6 cells), lung tissues (50 mg) or BMMCs (1 × 10 6 cells) were homogenized in lysis buffer, and then each protein was performed using immunoblotting (see additional file for details).…”

Section: Methodsmentioning

confidence: 99%

“…PCR assays were performed for 35 cycles. PCR products were analyzed using 1.0% agarose gel containing ethidium bromide (EtBr) [31] (see additional file for details).…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells

et al. 2011

Self Cite

View full text Add to dashboard Cite

BackgroundMany studies have found that smoking reduces lung function, but the relationship between cigarette smoke and allergic asthma has not been clearly elucidated, particularly the role of mast cells. This study aimed to investigate the effects of smoke exposure on allergic asthma and its association with mast cells.MethodsBALB/c mice were sensitized and challenged by OVA to induce asthma, and bone marrow-derived mast cells (BMMCs) were stimulated with antigen/antibody reaction. Mice or BMMCs were exposed to cigarette smoke or CSE solution for 1 mo or 6 h, respectively. The recruitment of inflammatory cells into BAL fluid or lung tissues was determined by Diff-Quik or H&E staining, collagen deposition by Sircol assay, penh values by a whole-body plethysmography, co-localization of tryptase and Smad3 by immunohistochemistry, IgE and TGF-β level by ELISA, expressions of Smads proteins, activities of signaling molecules, or TGF-β mRNA by immunoblotting and RT-PCR.ResultsCigarette smoke enhanced OVA-specific IgE levels, penh values, recruitment of inflammatory cells including mast cells, expressions of smad family, TGF-β mRNA and proteins, and cytokines, phosphorylations of Smad2 and 3, and MAP kinases, co-localization of tryptase and Smad3, and collagen deposition more than those of BAL cells and lung tissues of OVA-induced allergic mice. CSE solution pretreatment enhanced expressions of TGF-β, Smad3, activities of MAP kinases, NF-κB/AP-1 or PAI-1 more than those of activated-BMMCs.ConclusionsThe data suggest that smoke exposure enhances antigen-induced mast cell activation via TGF-β/Smad signaling pathways in mouse allergic asthma, and that it exacerbates airway inflammation and remodeling.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…PCR assays were performed for 35 cycles. PCR products were analyzed using 1.0% agarose gel containing ethidium bromide (EtBr) [31] (see additional file for details).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

Statin use protective for chronic rhinosinusitis in a nationally representative sample of the United States

et al. 2019

View full text Add to dashboard Cite

Objectives/Hypothesis Statins have long been used in the management of cardiovascular disease for their lipid‐lowering properties. However, recent research suggests that statins may also have anti‐inflammatory effects via modulation of lipid‐containing enzymes and mediators, and therefore may have therapeutic value in the treatment of chronic rhinosinusitis (CRS). Study Design Retrospective database review. Methods The 2006 to 2015 National Ambulatory Medical Care Survey (NAMCS) data were queried to analyze the relationship between statin use and rates of CRS. CRS was indicated by the presence of an International Classification of Diseases, Ninth Revision code for CRS in one of the five diagnosis variables. Statin use was indicated by the presence of a statin medication in any of the 30 medication variables using the Multum Lexicon Drug Database, with newly prescribed medications excluded. Relevant demographic, socioeconomic, and comorbid factors were included in a multivariate logistic regression model, which accounted for the complex, stratified, multistage survey design of the NAMCS. Results There were 390,538 unweighted visit records used in the weighted analysis dataset, corresponding to 9,612,613,668 weighted visits. Statin use was associated with a decreased rate of CRS in both a univariate analysis (odds ratio [OR] = 0.53, P < .001) and the multivariate logistic regression accounting for comorbid, socioeconomic, and demographic factors (OR = 0.79, P = .030). Conclusions Statin use is associated with decreased rates of CRS based on a nationally representative sample of outpatient visits in the United States. This supports research that suggests statin medications may have protective properties against CRS, and further research is warranted into their potential therapeutic value for this indication. Level of Evidence NA Laryngoscope, 130:848–851, 2020

show abstract

Evaluation of Simvastatin and Bone Marrow-Derived Mesenchymal Stem Cell Combination Therapy on Airway Remodeling in a Mouse Asthma Model

Mohammadian

Sadeghipour

Kashani

et al. 2016

Lung

View full text Add to dashboard Cite

show abstract

Anti-inflammatory mechanism of simvastatin in mouse allergic asthma model

Cited by 120 publications

References 45 publications

Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells

Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells

Statin use protective for chronic rhinosinusitis in a nationally representative sample of the United States

Evaluation of Simvastatin and Bone Marrow-Derived Mesenchymal Stem Cell Combination Therapy on Airway Remodeling in a Mouse Asthma Model

Contact Info

Product

Resources

About