Anti-Inflammatory Mechanism of Walnut Meal Peptide/ Ellagic Acid Nanoparticles on LPS-Stimulated RAW264.7 Cells by Inhibition of NF-κB and MAPK Activation

Ling, Yuxi; Feng, Yanxia; Li, Ziqi; Cheng, Le; Zhang, Xuan; Ren, Difeng

doi:10.1007/s10989-023-10571-w

Int J Pept Res Ther

2023

DOI: 10.1007/s10989-023-10571-w

|View full text |Cite|

Anti-Inflammatory Mechanism of Walnut Meal Peptide/ Ellagic Acid Nanoparticles on LPS-Stimulated RAW264.7 Cells by Inhibition of NF-κB and MAPK Activation

Yuxi Ling,

Yanxia Feng,

Ziqi Li

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Silkworm pupa protein-derived peptides alleviate LPS-induced inflammatory response in RAW264.7 macrophage cells through the NF-κB/MAPK/PI3K-AKT signaling pathway

Zhou,

Wang,

Guo

et al. 2024

Journal of Agriculture and Food Research

View full text Add to dashboard Cite

Silkworm pupa protein-derived peptides alleviate LPS-induced inflammatory response in RAW264.7 macrophage cells through the NF-κB/MAPK/PI3K-AKT signaling pathway

Zhou,

Wang,

Guo

et al. 2024

Journal of Agriculture and Food Research

View full text Add to dashboard Cite

Novel Peptides LFLLP and DFFL from Jack Bean Protein Hydrolysates Suppress the Inflammatory Response in Lipopolysaccharide-Stimulated RAW 264.7 Cells

Wijatniko,

Ishii,

Hirayama

et al. 2024

Foods

View full text Add to dashboard Cite

The production of inflammatory cytokines such as tumor necrosis factor (TNF)-α by activated macrophage cells plays an important role in the development of intestinal inflammation. The present study investigated the anti-inflammatory effect of the protein hydrolysates prepared from the jack bean (JBPHs), Canavalia ensiformis (L.) DC, using the enzyme Alcalase, in a murine macrophage model, RAW 264.7 cells, which were stimulated by lipopolysaccharides. JBPHs reduced the TNF-α expression at the protein and mRNA levels through the downregulation of cellular signaling pathways involved in nuclear factor kappa B (NF-κB), extracellular signal-regulated kinase (ERK), and p38. A combination of mass spectrometry and in silico approaches identified 10 potential anti-inflammatory peptides in the JBPHs, including LFLLP and DFFL. Interestingly, while LFLLP targeted the NF-κB pathway, DFFL targeted p38 and ERK to suppress the TNF-α production in the RAW 264.7 cells. In addition, LFLLP and DFFL were localized in the cytosol of the cells. These results demonstrated that LFLLP and DFFL were incorporated by RAW 264.7 cells and, at least in part, contributed to the reduction in TNF-α by JBPHs. These peptides isolated from JBPHs may well be utilized as new alternatives to alleviate intestinal inflammation.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Anti-Inflammatory Mechanism of Walnut Meal Peptide/ Ellagic Acid Nanoparticles on LPS-Stimulated RAW264.7 Cells by Inhibition of NF-κB and MAPK Activation

Cited by 2 publications

References 37 publications

Silkworm pupa protein-derived peptides alleviate LPS-induced inflammatory response in RAW264.7 macrophage cells through the NF-κB/MAPK/PI3K-AKT signaling pathway

Silkworm pupa protein-derived peptides alleviate LPS-induced inflammatory response in RAW264.7 macrophage cells through the NF-κB/MAPK/PI3K-AKT signaling pathway

Novel Peptides LFLLP and DFFL from Jack Bean Protein Hydrolysates Suppress the Inflammatory Response in Lipopolysaccharide-Stimulated RAW 264.7 Cells

Contact Info

Product

Resources

About