Anti-inflammatory potential of baicalein combined with silk fibroin protein in a zebrafish model (Danio rerio)

Abstract

Baicalein (BA) is a flavonoid with wideranging pharmacological activity. However, its biological evaluation is hampered by its low solubility in aqueous medium, making forms of incorporation that improve its solubility necessary. In the present study, BA was combined with a solution of silk fibroin protein (SF), a biomaterial used too as a drug carrier, to evaluate the anti-inflammatory potential of this combination, in vivo, in an experimental model, zebrafish (Danio rerio). Baicalein-silk fibroin (BASF) improved the DPPH (2,2-diphenyl-1-picrylhydrazyl-hydrate) free radical scavenging rate (95%) in comparison with BA in solution. The acute toxicity study and histopathological analysis in zebrafish showed that BASF has low cytotoxic potential, except for the maxim dose of 2000 mg/kg. The use of BA in combination with SF enhanced the anti-inflammatory effect of flavonoids by inducing inflammatory peritoneal edema through carrageenan and achieved 77.6% inhibition of abdominal edema at a dose of 75 mg/kg. The results showed that the BASF, significantly increases the bioavailability and therapeutic effect of flavonoids and several results observed in this study may help in the development of new drugs.

Larvicidal properties of terpenoid-based nanoemulsions against the dengue vector Aedes aegypti L. and their potential toxicity against non-target organism

“…These alterations may lead to changes in body weight, food consumption, behavior, blood circulation, and reproductive functions [65]. Everds et al (2013) state however that in toxicity trials, it is common to observe signs of stress in animals [66] and these histological alterations are considered normal, and not able to alter the normal functioning of the fish organs [67,68]. Although it is unlikely that pcymene and myrcene pose a significant hazard to non-target organisms, there is a need to assess their potential toxicity in simulated field conditions using the doses that may be applied in the field.…”

Diclofenac, ibuprofen, and paracetamol biodegradation: overconsumed non-steroidal anti-inflammatories drugs at COVID-19 pandemic

“…In most cases, the inflammation resolution occurs and the body returns to homeostasis after elimination. However, persistence of inflammatory cause and inefficient resolution can culminate in an exacerbated and prolonged inflammatory response that plays a key role in the development of multiple diseases ( Badri et al, 2016 ; Feehan and Gilroy, 2019 ; Holanda et al, 2023 ).…”

The baicalein amorphous solid dispersion to enhance the dissolution and bioavailability and effects on growth performance, meat quality, antioxidant capacity and intestinal flora in Taihang chickens

“…Baicalein ( BAI ),which is a natural flavonoid derived from the traditional Chinese herb, Scutellaria baicalensis (aka Huangqin) ( Markowski et al, 2023 ), has antioxidant ( Bayram et al, 2023 ), anti-inflammatory ( Holanda et al, 2022 ), antibacterial ( Yun et al, 2012 )and other pharmacological effects. The total antioxidant capacity ( T-AOC ) activity, total superoxide dismutase ( T-SOD ) and glutathione peroxidase ( GSH-Px ) level in liver of broilers was significantly increased by inclusion of BAI, suggested that dietary supplementation of BAI can improve the antioxidant ability of broilers ( Zhou et al, 2019 ).…”