Anti-Inflammatory Potential of Hecogenin in Experimental Animals: Possible Involvement of Inflammatory Cytokines and Myeloperoxidase

Abstract

Hecogenin is a steroidal sapogenin plays important role in treatment of variety of inflammatory diseases. We have investigated the anti-inflammatory effects of Hecogenin (50 µg/animal) (HG), Fluticasone (50 µg/animal) (FC) and Hecogenin+Fluticasone (HG+FC) combination (25 µg/animal, each) on various inflammatory models. The anti-inflammatory effect of HG, FC and HG+FC combination was studied on % inhibition of dry weight of granuloma tissue, Δ ear weight, myeloperoxidase assay, serum pro-inflammatory cytokines, colon weight to length ratio, macroscopic lesions, adhesion score, diarrhoea score and histopathological analysis of ear and colon tissue on Cotton pellets induced granuloma in rats, Croton oil induced ear edema in mice and TNBS induced granuloma in rats. Topical administration of HG and its combination with FC showed significant decrease (p<0.001) in the % inhibition of dry weight of granuloma tissue, Δ ear weight, myeloperoxidase level, serum pro-inflammatory cytokines levels, colon weigh to length ratio as compared with Cotton pellets treated with acetone groups and Croton oil treated animals. Further histopathological analysis of ear tissue showed significant decrease in dermal thickness and epidermal hyperplasia and colon tissue showed reduction of edema, infiltration of inflammatory cells and normalization of crypt structure compared to DC animals. Thus, the findings of present study suggest the possible role of HG in the treatment of inflammation by reducing the dose of FC in combination with HG.

Saponins and Sapogenins of Agave With Respect to Diverse Pharmacological Role of Hecogenin

“…Further, it also suppressed the myeloperoxidase and serum levels of Tumour necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) in cotton pellets induced granuloma model in rat. The result was further supported by histopathological analysis of ear tissue that showed significant decrease in dermal thickness and epidermal hyperplasia of ear tissue thus confirming its anti-inflammatory activity [16] ( fig. 3).…”

“…In addition to this, hecogenin exhibits variety of important biological activities in the pharmaceutical industry [12,13]. The steroidal sapogenins (hecogenin) from Agave genus were reported to have a number of pharmacological activities such as anti-inflammatory, analgesic, antiarthritis, gastroprotective, anticancer and larvicidal activity [14][15][16][17][18]. Overall, the results of numerous pharmacological studies have been linking the possible use of hecogenin as a novel multi-target based therapeutic agent against abundant long-lasting disease conditions.…”

Identification of hub genes and small-molecule compounds related to intracerebral hemorrhage with bioinformatics analysis

“…Although the two aforementioned drugs have been reported to be associated with the treatment of ICH, the mechanisms underlying their therapeutic actions still require further research. In addition, we found that hecogenin has an anti-inflammatory effect that may contribute to the treatment of ICH (Ingawale, Mandlik & Patel, 2019; Ingawale & Patel, 2016; Ingawale & Patel, 2018).…”

Peer Review #1 of "Identification of hub genes and small-molecule compounds related to intracerebral hemorrhage with bioinformatics analysis (v0.2)"

“…Although the two aforementioned drugs have been reported to be associated with the treatment of ICH, the mechanisms underlying their therapeutic actions still require further research. In addition, we found that hecogenin has an antiinflammatory effect that may contribute to the treatment of ICH (Ingawale et al 2019;Ingawale & Patel 2016;Ingawale & Patel 2018).…”