Anti-Inflammatory Properties of Flavone di-C-Glycosides as Active Principles of Camellia Mistletoe, Korthalsella japonica

Abstract

The chemical components and biological activity of Camellia mistletoe, Korthalsella japonica (Loranthaceae) are relatively unknown compared to other mistletoe species. Therefore, we investigated the phytochemical properties and biological activity of this parasitic plant to provide essential preliminary scientific evidence to support and encourage its further pharmaceutical research and development. The major plant components were chromatographically isolated using high-performance liquid chromatography and their structures were elucidated using tandem mass spectrometry and nuclear magnetic resonance anlysis. Furthermore, the anti-inflammatory activity of the 70% ethanol extract of K. japonica (KJ) and its isolated components was evaluated using a nitric oxide (NO) assay and western blot analysis for inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Three flavone di-C-glycosides, lucenin-2, vicenin-2, and stellarin-2 were identified as major components of KJ, for the first time. KJ significantly inhibited NO production and reduced iNOS and COX-2 expression in lipopolysaccharide-stimulated RAW 264.7 cells at 100 μg/mL while similar activity were observed with isolated flavone C-glycosides. In conclusion, KJ has a simple secondary metabolite profiles including flavone di-C-glycosides as major components and has a strong potential for further research and development as a source of therapeutic anti-inflammatory agents.

Phenolic distribution, antioxidant activity, and enzyme inhibitory properties of eggplant ( Solanum aethiopicum ) cultivated in two different locations within Nigeria

“…This could be due to the differences in temperature of two regions where samples were obtained. Interestingly, previous reports have highlighted numbers of beneficial health roles of these compounds, namely; antioxidative, antihypertensive, antidiabeti,c and hypocholesterolemic activities among others (Kim, Yun, Lim, Kim, & Jang, ; Oyeleye et al, ). It is important to note that chlorogenic acid is one of most abundant phenolic compounds present in S. aethiopicum .…”

TET-2 up-regulation is associated with the anti-inflammatory action of Vicenin-2

“…Flavone-C-glycosides are a class of naturally-occurring compounds known to have diverse anti-inflammatory actions [1]. Vicenin-2, a C-glycoside that is present in many plant sources, has been shown to exert potent anti-inflammatory effects both in vitro [2] and in an experimental colitis model [3].…”

Inhibitory Activities of Camellia Mistletoe (K. Japonica) Extracts on Pancreatic Lipase, Tyrosinase and Cancer Cell Proliferation

“…Mistletoe extracts are used for the treatment of many diseases such as cancer, hypertension, and diabetes [3][4][5]. Camellia mistletoe, Korthalsella japonica (family Santalaceae), is a semiparasitic plant which is distributed in Jeju Island, Korea.…”