Anti-Inflammatory Property of Biomaterial Carotenoids Production by Rhodobacter Sphaeroides WL-APD911

Abstract

A Rhodobacter sphaeroides WL-APD911 with the capability of producing the end products of neurosporene and ξ-carotene, rather than the normal end products of spheroidene, was isolated from hundreds of strains by using chemical mutagenesis. The strain WL-APD911 grew well in LB medium, and the colored carotenoids were produced within 3 days of incubation at 30°C. The Rs-M of colored carotenoid extracts obtained from strain WL-APD911 by using methanol extraction inhibited the NO production and iNOS expression by LPS-induced RAW 264.7 cells. This probability of reason for the Rs-M as the best source was to prompt the anti-inflammation and anti-oxidation in nutraceutical products. Finally, the carotenoid extracts (Rs-M) with neurosporene and ξ-carotene was called lycogene to be a newly biomaterial.

The Extract of Rhodobacter sphaeroides Inhibits Melanogenesis through the MEK/ERK Signaling Pathway

“…Meanwhile, neurosporene itself has the ability to protect irradiation with UV-B [10]. Lycogen™ inhibited NO production and iNOS expression in activated macrophages [3] and was capable of improving colonic damage in DSS-induced colitis [4]. Further work is warranted to elucidate the active ingredient(s) in Lycogen™ for hypopigmentation.…”

“…Some probiotics have immunomodulatory activities and modulate the symptoms of several diseases. Previously, we found that the extracts of Rhodobacter sphaeroides (Lycogen™) inhibited nitric oxide production and inducible nitric-oxide synthase expression in activated macrophages [3]. Meanwhile, the effect of Lycogen™, a potent anti-inflammatory agent, was evaluated in mice with dextran sodium sulfate (DSS)-induced colitis.…”

Extract from a mutant Rhodobacter sphaeroides as an enriched carotenoid source

“…A mutant strain capable of producing colored carotenoids, in contrast to the colorless phenotype of the wild type, was isolated from among hundreds of strains using a chemical mutagen sodium azide (NaN 3 ) ( 18 ) ( Fig. 1 ).…”

“…Lycogen™ has attracted significant attention because of its promising biotechnological potential as demonstrated in our previous studies ( 18 – 20 ). For example, Wu and Liu reported that R. sphaeroides (WL-APD911) grew well in LB medium, and the extract Lycogen™ had the ability to inhibit NO production and iNOS expression in LPS-induced RAW 264.7 cells.…”

“…Rhodobacter sphaeroides is one kind of photosynthetic bacterium, containing crtC gene and producing carotenoids. Lycogen™ is the methanol extract of a mutant strain, R. sphaeroides (WL-APD911) whose crtC gene has been altered by random chemical mutagenesis, leading to the production of a new carotenoid ( 18 ).…”