Anti-inflammatory property of quercetin through downregulation of ICAM-1 and MMP-9 in TNF-α-activated retinal pigment epithelial cells

Cheng, Shu-Chen; Wu, Yi-Hong; Huang, Wen‐Chung; Pang, Jong‐Hwei S.; Huang, Ting‐Hsiang; Cheng, Ching‐Yi

doi:10.1016/j.cyto.2019.01.001

Cited by 60 publications

(47 citation statements)

References 49 publications

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“…We identified 10 hub genes associated with neovascularization: TNF, VEGFA, PIK3CB, TGFB1, EDN1, MMP9, TLR4, PDGFB, MMP2, and THBS1. TNF, tumour necrosis factor, activates angiogenic factors, such as endothelin (EDN) and matrix metalloproteinases (MMPs), to mediate the progression of angiogenesis [33]. The overexpression of EDN1 may lead to the aberrant haemodynamics in the early DR. [34] The results of our study suggest that TNF and EDN1 jointly participate in the processes of cell proliferation, regulation of MAP kinase activity, and TNF signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of angiogenesis-related genes and pathways in early diabetic retinopathy

Lhamo

Zou

et al. 2020

BMC Med Genomics

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Background Angiogenesis is an important parameter in the development of diabetic retinopathy (DR), and it is indicative of an early stage evolving into a late phase. Therefore, examining the role of angiogenic factors in early DR is crucial to understanding the mechanism of neovascularization. Methods The present study identified hub genes and pathways associated with angiogenesis in early DR using bioinformatics analysis. Genes from published literature and Gene Expression Omnibus (GEO) were collected and analysed. Results We collected 73 genes from 70 published studies in PubMed, which were referred to as DR-related gene set 1 (DRgset1). The gene expression profile of GSE12610 was downloaded, and 578 differentially expressed genes (DEGs) between diabetic and normal samples were identified. DEGs and DRgset1 were further combined to create DR-related gene set 2 (DRgset2). After an enrichment analysis, we identified 12 GO terms and 2 pathways associated with neovascularization in DRgset1, and 8 GO terms and 2 pathways in DRgset2. We found 39 new genes associated with angiogenesis and verified 8 candidate angiogenesis-related genes in DR cells using real-time PCR: PIK3CB, ALDH3A1, ITGA7, FGF23, THBS1, COL1A1, MAPK13, and AIF1. We identified 10 hub genes associated with neovascularization by constructing a protein-protein interaction (PPI) network: TNF, VEGFA, PIK3CB, TGFB1, EDN1, MMP9, TLR4, PDGFB, MMP2, and THBS1. Conclusions The present study analysed angiogenesis-related genes and pathways in early DR in a comprehensive and systematic manner. PIK3CB, ALDH3A1, ITGA7, FGF23, THBS1, COL1A1, MAPK13, and AIF1 may be the candidate genes to further explore the mechanisms of angiogenesis in early DR. TNF, PIK3CB, TGFB1, EDN1, MMP9, TLR4, PDGFB, MMP2, and THBS1 may be new targets for early neovascularization therapy in the future.

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Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of angiogenesis-related genes and pathways in early diabetic retinopathy

Lhamo

Zou

et al. 2020

BMC Med Genomics

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“…It has anti-inflammatory, antioxidant, anti-apoptotic, and anticancer properties [13]. Studies reported by Cheng et al [14] showed that quercetin could be used in the treatment of human retinal inflammatory diseases. Chlorogenic acid is present mainly in coffee and tea, as well as in grape wines, tropical fruits, cabbages, and root vegetables [15].…”

Section: Mode Of Inhibition Ki [Mm] Vmax [δAu/min]mentioning

confidence: 99%

“…Thyroid peroxidase, also called thyroperoxidase (TPO, EC 1.11.1. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] or iodide peroxidase, is an enzyme participating in the synthesis of thyroid hormones. In the human body, it is encoded by the TPO gene located on chromosome 2p25 [1].…”

Section: Introductionmentioning

confidence: 99%

Thyroid Peroxidase Activity is Inhibited by Phenolic Compounds—Impact of Interaction

et al. 2019

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The aim of this study was to estimate the mode of thyroid peroxidase (TPO) inhibition by polyphenols: Chlorogenic acid, rosmarinic acid, quercetin, and rutin. All the tested polyphenols inhibited TPO; the IC50 values ranged from 0.004 mM to 1.44 mM (for rosmarinic acid and rutin, respectively). All these pure phytochemical substances exhibited different modes of TPO inhibition. Rutin and rosmarinic acid showed competitive, quercetin—uncompetitive and chlorogenic acid—noncompetitive inhibition effect on TPO. Homology modeling was used to gain insight into the 3D structure of TPO and molecular docking was applied to study the interactions of the inhibitors with their target at the molecular level. Moreover, the type and strength of mutual interactions between the inhibitors (expressed as the combination index, CI) were analyzed. Slight synergism, antagonism, and moderate antagonism were found in the case of the combined addition of the pure polyphenols. Rutin and quercetin as well as rutin and rosmarinic acid acted additively (CI = 0.096 and 1.06, respectively), while rutin and chlorogenic acid demonstrated slight synergism (CI = 0.88) and rosmarinic acid with quercetin and rosmarinic acid with chlorogenic acid showed moderate antagonism (CI = 1.45 and 1.25, respectively). The mixture of chlorogenic acid and quercetin demonstrated antagonism (CI = 1.79). All the polyphenols showed in vitro antiradical ability against 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), ABTS. The highest ability (expressed as IC50) was exhibited by rosmarinic acid (0.12 mM) and the lowest value was ascribed to quercetin (0.45 mM).

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“…The increasing interest in anti-inflammatory foods has also boosted research on natural functional pigments with anti-inflammatory effects because such pigments can be used as food coloring agents, in addition. The best-known examples of natural functional pigments with anti-inflammatory properties are quercetin [ 16 , 17 ], curcumin [ 18 , 19 ], anthocyanin [ 20 , 21 ], and Monascus pigments [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Anti-Inflammatory Monascus Pigment Derivatives

Choe

Song

Shin

et al. 2020

Foods

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The prevention and treatment of chronic inflammation using food-derived compounds are desirable from the perspectives of marketing and safety. Monascus pigments, widely used as food additives, can be used as a chronic inflammation treatment. Orange Monascus pigments were produced by submerged fermentation in a 5 L bioreactor, and multiple orange Monascus pigment derivatives with anti-inflammatory activities were synthesized using aminophilic reaction. A total of 41 types of pigment derivatives were produced by incorporating amines and amino acids into the orange pigments. One derivative candidate that inhibited nitric oxide (NO) production in Raw 264.7 cells and exhibited low cell cytotoxicity was identified via in vitro assay. The 2-amino-4 picoline derivative inhibited NO production of 48.4%, and exhibited cell viability of 90.6%. Expression of inducible NO synthase, an important enzyme in the NO synthesis pathway, was suppressed by such a derivative in a dose-dependent manner. Therefore, this derivative has potential as a functional food colorant with anti-inflammatory effects.

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Anti-inflammatory property of quercetin through downregulation of ICAM-1 and MMP-9 in TNF-α-activated retinal pigment epithelial cells

Cited by 60 publications

References 49 publications

Comprehensive analysis of angiogenesis-related genes and pathways in early diabetic retinopathy

Comprehensive analysis of angiogenesis-related genes and pathways in early diabetic retinopathy

Thyroid Peroxidase Activity is Inhibited by Phenolic Compounds—Impact of Interaction

Production and Characterization of Anti-Inflammatory Monascus Pigment Derivatives

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