Anti-inflammatory sesquiterpenoids from the Traditional Chinese Medicine Salvia plebeia : Regulates pro-inflammatory mediators through inhibition of NF-κB and Erk1/2 signaling pathways in LPS-induced Raw264.7 cells

Zou, Yi-Hong; Zhao, Liang; Xu, You-Kai; Bao, Jing‐Mei; Liu, Xin; Zhang, Junsheng; Li, Wei; Ahmed, Abrar; Yin, Sheng; Tang, Gui‐Hua

doi:10.1016/j.jep.2017.08.034

Cited by 68 publications

(38 citation statements)

References 52 publications

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“…As shown in ▶ Fig. 5, compound 5 exhibited about a 2-fold more potent inhibition on NF-κB activation (IC 50 = 11.0 µM) than the positive control pyrrolidine dithiocarbamate (PDTC), a well-known NF-κB inhibitor (IC 50 = 20.9 µM) [25], while compound 1 (IC 50 = 17.9 µM) was comparable to PDTC. This study demonstrated that the effects of 1 and 5 on the production of NO, TNF-α, and IL-6 in LPS-induced RAW264.7 macrophages were associated with the inhibition of NF-κB transcriptional activity.…”

Section: Resultsmentioning

confidence: 97%

“…Compounds 1-19 were evaluated for their inhibitory effects on NO production in LPS-activated RAW264.7 macrophages. Quercetin, a well-known natural NO inhibitor, was used as the positive control (IC 50 = 15.8 µM) [25]. As shown in ▶ Table 1, compounds 1-6, 10-13, and 19 exhibited pronounced inhibitory activity with IC 50 values ranging from 2.78 to 10.6 µM, while the others with IC 50 values greater than 25 µM were considered to be inactive.…”

Section: Resultsmentioning

confidence: 99%

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Anti-inflammatory Ingenane Diterpenoids from the Roots of Euphorbia kansui

et al. 2018

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Bioassay-guided fractionation of the ethanolic extract of the roots of led to the isolation of two new ingenane diterpenoids, euphorkans A (1: ) and B (2: ), together with 16 known analogues (3: - 18: ). Their structures were determined by combined spectral and chemical methods. All the isolates were evaluated for their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophage cells. Compounds 1: - 6: and 10: - 13: exhibited pronounced inhibitory activity with IC values in the range of 2.78 - 10.6 µM, and were more potent than the positive control, quercetin (IC = 15.8 µM). Compounds 1: and 5: were selected for further assays toward the key inflammation mediators TNF- and IL-6, and showed a significant inhibition in a dose-dependent manner. The preliminary mechanistic study revealed that 1: and 5: inhibited NF-B activity, which may exert a role in their anti-inflammatory activity.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory Ingenane Diterpenoids from the Roots of Euphorbia kansui

et al. 2018

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“…Studies have shown that inhibition of NF-kB activity have great benefits in the treatment of inflammatory diseases (including AD) [32][33][34]. Combined with the 'Ab hypothesis' and the 'inflammatory response theory', we propose that the mechanism of VTP on AD may be mediated through the NF-kB/IkB-a signalling pathway.…”

Section: Effect Of Vtp On the Mrna Level Of The Nf-kb/ikb-a Signalingmentioning

confidence: 86%

“…Aggregation of Ab plays an important role in the pathogenesis of AD [22,23]. In this study, the animal model of AD was established using Ab [25][26][27][28][29][30][31][32][33][34][35] , the core fragment of Ab 1-42 [24]. The Water maze test showed that the learning and memory ability of the model group decreased significantly, and AD-like manifestations were found.…”

Section: Effect Of Vtp On the Mrna Level Of The Nf-kb/ikb-a Signalingmentioning

confidence: 99%

Effect of polysaccharides from Vitis vinifera L. on NF-κB/IκB-α signal pathway and inflammatory factors in Alzheimer's model rats

Wang

et al. 2018

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the mechanism of action underlying the effect of polysaccharide from Vitis vinifera L. (VTP) on the nuclear factor kappa B/inhibitor kappa B alpha (NF-kB/IkB-a) signaling pathway and related inflammatory factors in hippocampus of rats with Alzheimer's disease (AD). Amyloid-b 25-35 was injected into the hippocampus to establish AD model rats, and the rats were administered with donepezil and VTP. The levels of interleukin-1b, interleukin-6 and tumour necrosis factor-a in the serum were determined by enzyme-linked immunosorbent assay. The phosphorylation level, protein and gene expression of NF-kBp65 and IkB-a in hippocampus were detected by immunohistochemistry, western blot and real-time quantitative polymerase chain reaction (PCR), respectively. VTP effectively improved the learning and memory ability of AD rats. Transmission electron microscopy indicated that VTP reduced the toxic effects of amyloid-b 25-35 on neurons in AD rats. Enzyme-linked immunosorbent assay showed that VTP inhibited the expression of interleukin-1b, interleukin-6 and tumour necrosis factor-a in a dosedependent manner. Compared with the model group, the expression levels of NF-kBp65 in the nucleus of the VTP group decreased, which was consistent with the Western blotting results. The expression of p-IkB-a and IkB-a, and, the mRNA level of NF-kBp65 and IkB-a in VTP group were significantly decreased compared to that in the model group, indicating that VTP has a therapeutic effect on AD. Its mechanism may be related to the inhibition of inflammatory response.

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“…The excessive production of pro-inflammatory mediators and cytokines, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumour necrosis factor-a (TNF-a), interleukin (IL)-1b, and IL-6, by macrophages, can cause various chronic inflammatory diseases [33]. Therefore, we investigated whether GNP-CK-CopA3 affected the gene and protein expression of those factors.…”

Section: Mrna and Protein Expression Of Pro-inflammatory Factorsmentioning

confidence: 99%

Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophages

Liu

Perumalsamy

Kang

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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Probiotic Gluconacetobacter strains are intestinal microbes with beneficial effects on human health. Recently, researchers have used these strains to biosynthesize metal and non-metal nanoparticles for treating various chronic diseases. Despite their importance in nanotechnology, gold nanoparticles (AuNPs) biosynthesized by Gluconacetobacter species have not been clearly identified for treating inflammation and inflammation-associated diseases. While ginsenoside CK has strong pharmaceutical activity, it also has strong cytotoxicity and hydrophobicity which is hurdle to make formulation. Peptide-nanoparticle hybrids are gaining increasing attention for their potential biomedical applications, including human inflammatory diseases. Herein, we developed peptide CopA3 surface conjugated and ginsenoside compound K (CK) loaded gold nanoparticles (GNP-CK-CopA3), which intracellularly synthesised by the probiotic Gluconacetobacter liquefaciens kh-1, to target lipopolysaccharide (LPS)-activated RAW264.7 macrophages. The synthetic GNP-CK-CopA3 was characterised by various instrumental techniques. The results of our cellular uptake and MTT assays exhibited obvious drug intracellular delivery without significant cytotoxicity. In addition, pre-treatment with GNP-CK-CopA3 significantly ameliorated LPS-induced nitric oxide (NO) and reactive oxygen species (ROS) production and suppressed the mRNA and protein expression of pro-inflammatory cytokines in macrophages. Furthermore, GNP-CK-CopA3 efficiently inhibited the activation of the nuclear factor-jB (NF-jB) and mitogen-activating protein kinase (MAPK) signalling pathways. Taken together, our findings highlight the potential of using peptide-nanoparticle hybrids in the development of anti-inflammatory approaches and providing the experimental foundation for further application.

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Anti-inflammatory sesquiterpenoids from the Traditional Chinese Medicine Salvia plebeia : Regulates pro-inflammatory mediators through inhibition of NF-κB and Erk1/2 signaling pathways in LPS-induced Raw264.7 cells

Cited by 68 publications

References 52 publications

Anti-inflammatory Ingenane Diterpenoids from the Roots of Euphorbia kansui

Anti-inflammatory Ingenane Diterpenoids from the Roots of Euphorbia kansui

Effect of polysaccharides from Vitis vinifera L. on NF-κB/IκB-α signal pathway and inflammatory factors in Alzheimer's model rats

Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophages

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