Anti-Inflammatory Study of Anredera Cordifolia Leaves and Centella Asiatica Herbs and Its Combinations Using Human Red Blood Cell-Membrane Stabilization Method

Abstract

Objective: Inflammation is body reactions in response to tissue injury and infection. In 2011, non-steroidal anti-inflammatory drug (NSAID) was thehighest demand drug in Indonesia. However, long-term treatment using NSAID can cause several side effects to cardiovascular and digestive system.This research aimed to investigate anti-inflammatory properties of binahong leaves (Anredera cordifolia) and pegagan herbs (Centella asiatica).Methods: Ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs was evaluated for its anti-inflammatory properties using human redblood cell (RBC) – membrane stabilization assay. The extract concentrations used in this study was 100, 200, 400, and 800-ppm, and apigenin andasiaticoside concentration were 1, 2, 3, 4, 5, 6, 10, and 100 ppm. Diclofenac natrium (DN) was used as a standard drug.Results: The results showed that A. cordifolia extract (ACE) alone, C. asiatica extract (CAE) alone, and the combination of ACE and CAE could inhibit thehemolysis of RBC in hypotonic solution. The optimum concentration for ACE alone was 100 ppm; for CAE alone was 400 ppm; and for the combinationof ACE and CAE was 50 ppm and 50 ppm, respectively. Apigenin and asiaticoside in concentration of 1-10 ppm showed more than 97% inhibition ofhemolysis. DN as a standard drug showed optimum inhibition at concentration of 400 ppm.Conclusion: The ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs showed anti-inflammatory activity, both as a single treatment or ascombinations, and apigenin and asiaticoside were responsible for anti-inflammatory activity in C. asiatica.Keywords: Anti-inflammation, Human red blood cell – membrane stabilization, Anredera cordifolia, Centella asiatica.

Effect of Diabetes Condition on Topical Treatment of Binahong Leaf Fraction in Wound Healing Process

“…Madecassol® berasal dari ekstrak tanaman Centella asiatica (pegagan) yang mengandung zat aktif asiaticoside, madecassic acid, dan asiatic acid yang berperan dalam penyembuhan luka (Shukla et al, 1999). Binahong dan pegagan keduanya memiliki aktivitas sebagai antiinflamasi yang juga berperan dalam mekanisme penyembuhan luka (Sutrisno et al, 2016 Kelompok perlakuan: kontrol normal (non diabetes), kontrol negatif (diabetes + aplikasi topikal biocream®), kontrol positif (diabetes + aplikasi topikal madecassol), aplikasi topikal 5 jenis fraksi daun binahong: FHDB (fraksi heksan), FKDB (fraksi kloroform), FEADB (fraksi etil asetat), FEDB (fraksi etanol), FADB (fraksi air) konsentrasi 5% dan 10% dengan basis biocream®.…”

In vitro anti-inflammatory activity of aqueous, ethanolic and ethereal extracts of rhizomes, leaves and stems of Anredera vesicaria

“…These results are comparable with the anti-inflammatory activity found in other plants of the same genus. 11 The membrane stabilizer effect could be attributed to the presence of oleanolic acid, 4 a triterpenoid with proved wound-healing 12 and anti-inflammatory properties. [13][14][15] The quantitative factors studied and their interactions on the interest responses were also studied (Figure 1).…”

Anti-Inflammatory Properties of Pavetta Crassicaulis Bremek. Leaf and Flower Crude Extracts and Its Pure Compounds Collected From Western Ghats, Karnataka, India

“…Some of the researchers used different methods of anti-inflammatory activity such as croton oil-induced ear edema in rats [80,81], protein denaturation bioassay [82][83][84][85][86], membrane stabilization assay using human red blood cell [87,88], but most of the researchers followed carrageenan-induced rat paw edema [89][90][91][92][93] to evaluated antiinflammatory activity.…”