Anti-?-inhibin

Fetsch, A M T Patricia; Powers, Celeste N.; Zakowski, Maureen F.; Abati, Andrea

doi:10.1002/(sici)1097-0142(19990625)87:3<168::aid-cncr11>3.0.co;2-v

Cited by 53 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anecdotally, we have seen a few cases of adrenal-renal fusion initially interpreted as CC-RCC on biopsy. Although there are many previous studies of immunohistochemical markers used for the diagnosis of RCC, 2-6, 9-12, 16, 25, 27, 33, 34, 36, 41, 42, 44, 45, 50, 53, 54 it is our experience that overlapping immunophenotypes are more commonly seen in routine practice than would be expected from the reported literature. Also, the appropriate diagnostic threshold for regarding a stain as “positive” has not been fully addressed in this setting.…”

Section: Discussionmentioning

confidence: 72%

“…Clinically, the occurrence of steroid-inactive adrenal cortical lesions may further confound histologic interpretation. 8, 30, 40 While earlier studies have compared immunostaining results in this setting, 2-6, 9-12, 16, 25, 27, 33, 34, 36, 41, 42, 44, 45, 50, 53, 54 our goal was to characterize the immunoprofile of primary lesions of the adrenal cortex and metastatic CC-RCC using tissue microarray technology to simulate the small biopsy samples obtained in routine practice, with a focus on the effect of varying intensity thresholds on sensitivity and specificity. In addition, the expression profile with anti-carbonic anhydrase IX (CAIX), anti-human kidney injury molecule-1 (hKIM-1), anti-hepatocyte nuclear factor-1b (HNF-1b), anti-PAX-8, and anti-T cell immunoglobulin mucin-1 (TIM-1), which are proposed markers of renal cell carcinoma 7, 13, 21, 23, 29, 47 that to our knowledge have not been fully characterized in adrenal cortical lesions, was studied to determine diagnostic utility in this differential diagnostic setting.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemical Distinction of Primary Adrenal Cortical Lesions From Metastatic Clear Cell Renal Cell Carcinoma

Sangoi

Fujiwara

West

et al. 2011

American Journal of Surgical Pathology

117

View full text Add to dashboard Cite

The diagnosis of metastatic clear cell renal cell carcinoma (CC-RCC) can be difficult because of its morphologic heterogeneity and the increasing use of small image-guided biopsies that yield scant diagnostic material. This is further complicated by the degree of morphologic and immunophenotypic overlap with non-renal neoplasms and tissues, such as adrenal cortex. In this study, a detailed immunoprofile of 63 adrenal cortical lesions, which included 54 cortical neoplasms, was compared with 185 metastatic CC-RCC using traditional [anti-calretinin, CD10, anti-chromogranin, anti-EMA, anti-inhibin, anti-melanA, anti-cytokeratins (AE1/AE3 and AE1/CAM5.2), anti-renal cell carcinoma marker (RCCma), and anti-synaptophysin)] and novel [anti-carbonic anhydrase-IX (CAIX), anti-hepatocyte nuclear factor-1b (HNF-1b), anti-human kidney injury molecule-1 (hKIM-1), anti-PAX-2, anti-PAX-8, anti-steroidogenic factor-1 (SF-1), and anti-T cell immunoglobulin mucin-1 (TIM-1)] antibodies. Tissue microarray methodology was used to simulate small image-guided biopsies. Staining extent and intensity were scored semiquantitatively for each antibody. In comparing different intensity thresholds required for a ‘‘positive’’ result, ≥2+ was identified as optimal for diagnostic sensitivity/specificity. For the distinction of adrenal cortical lesions from metastatic CC-RCC, immunoreactivity for the adrenal cortical antigens SF-1 (86% adrenal; 0% CC-RCC), calretinin (89% adrenal; 10% CC-RCC), inhibin (86% adrenal; 9% CC-RCC), and melanA (86% adrenal; 10% CC-RCC) and the renal epithelial antigens hKIM-1 (0% adrenal; 83% CC-RCC), PAX-8 (0% adrenal; 83% CC-RCC), HNF-1b (0% adrenal; 76% CC-RCC), EMA (0% adrenal; 78% CC-RCC), and CAIX (3% adrenal; 87% CC-RCC) had the most potential utility. The use of the novel renal epithelial markers hKIM-1 (clone AKG7) and/or PAX-8, and the adrenocortical marker SF-1 in an immunohistochemical panel for distinguishing adrenal cortical lesions from metastatic CC-RCC offers improved diagnostic sensitivity and specificity.

show abstract

Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Distinction of Primary Adrenal Cortical Lesions From Metastatic Clear Cell Renal Cell Carcinoma

Sangoi

Fujiwara

West

et al. 2011

American Journal of Surgical Pathology

117

View full text Add to dashboard Cite

show abstract

“…INHA immunoreactivity has been detected in adrenal cortical adenomas and carcinomas. Earlier studies showed positive staining for INHA in hyperplastic tissues and adrenocortical carcinomas (De Jong et al 1990), which was later supported by data from larger studies in patients with adrenal cortical neoplasia (Arola et al 1998, McCluggage et al 1998, Pelkey et al 1998, Fetsch et al 1999, Munro et al 1999. Similar to ovarian cancer, a subgroup of adrenal cortical carcinomas showed loss of INHA immunoreactivity (Munro et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Epigenetic regulation of inhibin alpha-subunit gene in prostate cancer cell lines

Balanathan¹,

Ball²,

Wang³

et al. 2004

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Inhibin was first identified as a gonad-derived regulator of pituitary FSH; however, it has subsequently been shown to be a tumour suppressor in the gonad and adrenal glands. Whereas non-malignant regions of human primary prostate carcinomas express inhibin -subunit (INHA), malignant tissues lack INHA transcript and protein, which is consistent with epigenetic regulation of the inhibin -subunit gene (INHA) promoter. This study investigated whether methylation of the INHA promoter was responsible for inactivation of INHA transcription and translation in the prostate cancer cell lines, LNCaP, DU145 and PC3. Methylation of the promoter was revealed by bisulphite genomic sequencing and use of inhibitors of methylation and histone deacetylation resulted in reactivation of the INHA transcription and translation. Significant (P<0·05) downregulation of a luciferase reporter gene downstream from a methylated INHA promoter compared with unmethylated INHA promoter occurred in vitro. The data demonstrate that promoter methylation is associated with downregulation of the INHA gene in prostate cancer cell lines, which is consistent with its tumour suppressive role. Therefore INHA has a significant role in prostate tumorigenesis.

show abstract

“…[910] Alfa-inhibin antibody shows an intense and specific immunostaining pattern for cells of adrenal origin, even in paucicellular samples, and there is a scope for widespread clinical utility of this marker by cytopathologists. [3]…”

Section: Discussionmentioning

confidence: 99%

“…To date, the immunohistochemical distinction of ACC from RCC is based on a panel of antibodies that include vimentin, cytokeratins and epithelial membrane antigen (EMA). [3]…”

Section: Introductionmentioning

confidence: 99%

Cytodiagnosis of myxoid adrenocortical carcinoma and role of immunocytochemistry to differentiate it from renal cell carcinoma

Mondal

Dasgupta

Mandal

et al. 2014

J Cytol

View full text Add to dashboard Cite

Adrenocortical carcinoma (ACC) is a rare malignancy and cytodiagnosis of this tumor is not routinely encountered by a cytopathologist. Here, we report a case of ACC initially diagnosed by computed tomography (CT)-guided fine needle aspiration cytology (FNAC) with the help of immunocytochemistry. A 48-year-old lady presented with flank pain and abdominal mass for the last 6 months. A CT scan of her abdomen revealed a large mass arising from the upper part of the left kidney. CT-guided FNAC was performed. Cytologic smears showed pleomorphic large cells arranged discretely and in small aggregates against a myxoid background. The cells had a high nucleocytoplasmic ratio, anisonucleosis and conspicuous nucleoli. Based on cytomorphology, differential diagnoses of ACC and renal cell carcinoma (RCC) were made. On immunocytochemistry, the tumor cells were synaptophysin, inhibin, vimentin and Melan-A positive but cytokeratin and epithelial membrane antigen negative. Thus, a cytodiagnosis of myxoid ACC was made and histopathologic examination was suggested. Subsequent histologic examination and immunohistochemistry proved the case to be myxoid ACC.

show abstract

Anti-?-inhibin

Cited by 53 publications

References 21 publications

Immunohistochemical Distinction of Primary Adrenal Cortical Lesions From Metastatic Clear Cell Renal Cell Carcinoma

Immunohistochemical Distinction of Primary Adrenal Cortical Lesions From Metastatic Clear Cell Renal Cell Carcinoma

Epigenetic regulation of inhibin alpha-subunit gene in prostate cancer cell lines

Cytodiagnosis of myxoid adrenocortical carcinoma and role of immunocytochemistry to differentiate it from renal cell carcinoma

Contact Info

Product

Resources

About