Anti-invasive and anti-adhesive activities of a recombinant disintegrin, r-viridistatin 2, derived from the Prairie rattlesnake (Crotalus viridis viridis)

Anti-angiogenic activities of two recombinant disintegrins derived from the Mohave and Prairie rattlesnakes

“…These data are consistent with previous studies by Scarborough et al (1993), where they tested the ability of different RGDNP and RGDW peptides to inhibit the binding of vitronectin to α v β 3 integrin; their results showed that RGDNP-containing peptides inhibited the binding of vitronectin to α v β 3 integrin more effectively than the RGDW-containing peptides. r-Viridistatin 2 in our study is a monomeric, medium size disintegrin with 73 amino acids, and an RGDNP-motif region derived from the Prairie rattlesnake (Lucena et al, 2012). r-Mojastin 1 is a monomeric, medium size disintegrin with 71 amino acids, twelve cysteines and an RGDW-motif region derived from the Mohave rattlesnake (Sánchez et al, 2010) (Fig.…”

“…Recombinant mojastin 1 and recombinant viridistatin 2 were expressed in E. coli and further purified by two-step chromatography using the method of Sánchez et al (2010)and Lucena et al (2012), respectively.…”

“…Recently, we have described the cloning and functional characterization of another recombinant disintegrin derived from Crotalus viridis viridis called r-viridistatin 2, which showed potent anti-metastatic activities against five different human tumoral cell lines. r-Viridistatin 2, efficiently inhibited various functions of the tumoral cells such as adhesion, migration, invasion and lung tumor colonization, with different potency depending of the tumoral cell line used (Lucena et al, 2012). …”

Disintegrins of Crotalus simus tzabcan venom: Isolation, characterization and evaluation of the cytotoxic and anti-adhesion activities of tzabcanin, a new RGD disintegrin

“…Disintegrin-induced apoptosis in HUVEC cells has been documented with rhodostomin [48] as well as with accutin [49]; however, the ability of disintegrins to induce apoptosis varies between cancer cell lines. Lucena et al [29] found that the recombinant RGDN disintegrin, r-viridistatin, failed to induce apoptosis in SK-12 melanoma cells, and the disintegrin rhodostomin did not induce apoptosis of MDA-MB-231 cells [50]. Similarly, the homodimeric disintegrin contortrostatin was found to lack cytotoxicity toward MDA-MB-435 cells in vitro [22].…”

“…Differential potency may be cell line dependent; for example, colombistatin had a potent inhibitory effect (IC 50 ¼ 33 nM) on SK-Mel-28 cell adhesion to fibronectin, yet much higher concentrations (IC 50 ¼ 4.4 mM) of this disintegrin were needed to inhibit T24 cells from binding to fibronectin [23]. A recombinant form of viridistatin-2 also showed varying binding inhibition of numerous cell lines to fibronectin, with IC 50 values ranging from 11 to 4450 nM [29]. Further, although inhibition of cell adhesion was not as potent as other disintegrins, tzabcanin may exhibit additional anti-metastatic properties by binding different integrin receptors.…”