Anti-Kappa/Lambda Immunobead for Detection of Human B Lymphocytes

Loren, Alan; Hendricks, Robert L.; Yokoyama, Mitsuo

doi:10.3109/08820137809068729

Cited by 6 publications

(2 citation statements)

References 7 publications

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“…The mean value and its (SD) of E-active and E-total rosettes with SRBC of 25.6% (10.6%) and 50.4% T (10.5%), respectively, was verified in this study [7], As shown in figure 1, the percentage of the EA rosettes using SRBC sensitized with different dilutions of hemolysin, even at ex ceedingly low concentrations, was largely unaffected although there was some fluctua tion (18-28%).…”

Section: Resultsmentioning

confidence: 84%

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Dose Response with Sensitizing Antibody in EA Rosette Formation

Loren¹,

Horita²,

Tseng³

et al. 1980

Int Arch Allergy Immunol

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With the exception of the EA rosette test using SRBC, all other assay systems using BRBC and HRBC showed a significant dose response in rosette-forming cell count when the sensitizing antibody was further diluted. The percent of rosette-forming cell counts of the EA assay with SRBC, however, was unaffected by dilutions of antiserum even at exceedingly high levels. The present results suggest using red cells, which are nonreactive with T lymphocytes for the assay of B lymphocytes, and the use of antibody for sensitization of red blood cells must be adjusted for the optimal dilution necessary for proper EA rosette formation.

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Section: Resultsmentioning

confidence: 84%

“…The normal pro cedure for the E-active assay requires 60 min of incubation which yields approxi mately 25.6% RFC [7]. Since all our incu bations were 15 min, this would explain the relative consistency of the percent RFC in the SRBC-EA rosette assay of approximate ly 22%.…”

Section: Discussionmentioning

confidence: 92%