Anti-L-selectin oligonucleotide ligands recognize CD62L-positive leukocytes: Binding affinity and specificity of univalent and bivalent ligands

Tenascin-C Aptamers Are Generated Using Tumor Cells and Purified Protein

“…Indeed, this occurred with the initial U251 cell selections that identified an ssDNA aptamer against TN-C, as well as the identification of a new 42-kDa trypanosome protein (54). A disadvantage of cell selections is that slow convergence of cell SELEX pools is caused by higher background binding of nucleic acids to cells (55) than to purified proteins. Furthermore, selection for high affinity may be compromised because concentration of the target protein(s) is unknown.…”

Polyvalent nucleic acid aptamers and modulation of their activity: a focus on the thrombin binding aptamer

“…Among the possible ligands, the engineering of multivalent nucleic acid aptamers for enhancing their performances is clearly attractive, and found many applications ranging from diagnostics (Borbas et al, 2007) to drug delivery (Wullner et al, 2008;Mallikaratchy et al, 2011) or the development of novel receptor agonists or antagonists (Ringquist & Parma, 1998). In particular, the multivalent version of aptamers targeting cell surface receptors has been demonstrated to efficiently modulate immune response in vivo, representing a valuable alternative to antibodies for cancer immunotherapy (Santulli-Marotto et al, 2003;Dollins et al, 2008;McNamara et al, 2008).…”

Aptamers for In Vivo Imaging

“…Making use of the SELEX process, RNA aptamers were generated binding to human L-selectin for use in imaging of inflammation (Ringquist and Parma, 1998). In vitro tests demonstrated that these aptamers preferentially recognize intracellular L-selectin with a high specificity and sensitivity.…”