Anti-<i>Anisakis</i> Antibodies in the Clinical Course of Crohn’s Disease

Abstract

Background: Human anisakidosis is a parasitic disease whose intestinal form shares several characterictics with Crohn’s disease (CD), like the ileocolonic location predominantly and the presence of granuloma. Methods: 73 CD patients diagnosed according to Lennard-Jones’ criteria were studied. The disease activity was measured by means of Harvey-Bradshaw Index. Different clinical variables were analyzed. Antigenic extract of Anisakis simplex larvae extracted from blue whiting was prepared. Determination of IgG, IgM, IgA and total specific immunoglobulin against A. simplex were carried out in sera by enzyme-linked immunosorbent assay. Immunorecognition patterns of these sera were realized by immunoblotting analysis. Results were compared with prevalence of antibodies anti-Anisakis in 251 healthy controls. Results: 29% of CD patients showed detectable specific total Ig (G+M+A) against A. simplex (95% CI, 19–41). For IgG, IgM and IgA the percentages were 44, 18 and 53% (95% CI, 32–56, 10–26, and 41–65) respectively. In positive specific total Ig cases CD was localized mainly in ileum. In IgG-, IgM- and IgA-positive cases the location was predominantly ileocolonic. CD activity index was higher for groups with positive IgA (2.86 vs. 5.55; p < 0.05). Conclusion: Patients with CD have high prevalence of specific immunoglobulin against A. simplex. Specific IgA are associated to higher activity index of CD.

Anisakis sensitization in different population groups and public health impact: A systematic review

“…Immunoblotting technique was performed along with IgE detection by means of previously cited tests in 4 studies on general asymptomatic population [ 30 , 14 , 48 , 49 ]; 4 studies on symptomatic population with allergies to any kind of allergen [ 14 , 30 , 31 , 50 ]; 1 study on patients presenting to hospital for controls [ 51 ] and 2 studies on patients with digestive system disorders [ 32 , 49 ].…”

Urticaria and silent parasitism by Ascaridoidea: Component-resolved diagnosis reinforces the significance of this association

“…The use of specific IgG antibodies to assess the anisakiasis seroprevalence and its possible association with other diseases, such as urticaria [50], Crohn's disease, gastrointestinal cancer, and others [59], has been reported in very few studies, and reference values for specific IgG antibodies against Anisakis antigens had not yet been defined. In our study, the establishment of normal values of specific IgG to Anisakis allowed to discriminate between individuals without urticaria and patients with urticaria, with special mention to the patients with the chronic urticaria.…”