Anti-melanogenic Activity of Auraptene via ERK-mediated MITF Downregulation

Kim, Min-Gon; Kim, Sang Suk; Park, Kyung-Jin; An, Hyun Joo; Choi, Young Hun; Lee, Nam Ho; Hyun, Chang‐Gu

doi:10.3390/cosmetics4030034

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Recent studies have indicated that 200 nM α‐MSH is normally used to stimulate B16F10 melanoma cells. Therefore, we used 200 nM α‐MSH to stimulate B16F10 melanoma cells 13–15 . Further experiments were conducted using these concentrations.…”

Section: Resultsmentioning

confidence: 99%

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Ligularia fischeri ethanol extract: An inhibitor of alpha‐melanocyte‐stimulating hormone‐stimulated melanogenesis in B16F10 melanoma cells

Lim

Joo

Choi

et al. 2022

J of Cosmetic Dermatology

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Background: Ligularia fischeri is a perennial herb isolated from plants of the Asteraceae family. Ligularia fischeri is distributed throughout Korea, Japan, eastern Siberia, and China. Aims:The aim of this study is to examine the intracellular inhibitory effect of Ligularia fischeri ethanol extract on melanin synthesis and expression of tyrosinase and tyrosinase-related protein 1 and 2. In addition, we analyzed the mitogen-activated protein kinase signaling pathway and microphthalmia-associated transcription factor in alpha-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells. Methods:To assess the inhibition of melanogenesis in alpha-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells, the expression of melanogenesisrelated genes was investigated by quantitative real-time polymerase chain reaction, while western blotting was performed to determine protein expression levels. Results:We confirmed that the ethanol extract of Ligularia fischeri inhibited melanin synthesis in vitro by decreasing tyrosinase and tyrosinase-related protein 1 and 2 expression. Furthermore, we revealed that tyrosinase expression was regulated by the suppression of microphthalmia-associated transcription factor expression and activation of extracellular signal-regulated kinase phosphorylation. The ethanol extract of Ligularia fischeri inhibited melanogenesis by activating extracellular signal-regulated kinase phosphorylation and suppressing microphthalmia-associated transcription factor and tyrosinase expression.Conclusions: Ligularia fischeri ethanol extract may be used as an effective skin whitening agent in functional cosmetics.

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Section: Resultsmentioning

confidence: 99%

“…Therefore, we used 200 nM α‐MSH to stimulate B16F10 melanoma cells. 13 , 14 , 15 Further experiments were conducted using these concentrations. Even when B16F10 melanoma cells were treated with α‐MSH and LFE, there was no significant difference compared with the non‐LFE‐treated group (Figure 1B ).…”

Section: Resultsmentioning

confidence: 99%

Ligularia fischeri ethanol extract: An inhibitor of alpha‐melanocyte‐stimulating hormone‐stimulated melanogenesis in B16F10 melanoma cells

Lim

Joo

Choi

et al. 2022

J of Cosmetic Dermatology

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“…During our ongoing screening program designed to identify modulators of skin inflammation and melanogenesis from coumarin and its derivatives, we reported that 8-methoxycoumarin increased melanogenesis via the MAPK signaling pathway [ 8 ]. In addition, we identified that auraptene, the most abundant naturally occurring geranyloxycoumarin, possesses anti-melanogenic activity through ERK-mediated MITF downregulation [ 9 ]. Furthermore, we reported that 7,8-dimethoxycoumarin stimulates melanogenesis via MAPK-mediated MITF upregulation and attenuates the expression of IL-6, IL-8, and CCL2/MCP-1 in TNF-α-treated HaCaT cells [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

4-Hydroxy-7-Methoxycoumarin Inhibits Inflammation in LPS-activated RAW264.7 Macrophages by Suppressing NF-κB and MAPK Activation

Kang

Hyun

2020

Molecules

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Coumarins are natural products with promising pharmacological activities owing to their anti-inflammatory, antioxidant, antiviral, anti-diabetic, and antimicrobial effects. Coumarins are present in many plants and microorganisms and have been widely used as complementary and alternative medicines. To date, the pharmacological efficacy of 4-hydroxy-7-methoxycoumarin (4H-7MTC) has not been reported yet. Therefore, in this study, we investigated the anti-inflammatory effects of 4H-7MTC in LPS-stimulated RAW264.7 cells as well as its mechanisms of action. Cells were treated with various concentrations of 4H-7MTC (0.3, 0.6, 0.9, and 1.2 mM) and 40 μM L-N6-(1-iminoethyl)-L-lysine (L-NIL) were used as controls. LPS-stimulated RAW264.7 cells showed that 4H-7MTC significantly reduced nitric oxide (NO) and prostaglandin E2 (PGE2) production without cytotoxic effects. In addition, 4H-7MTC strongly decreased the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2). Furthermore, 4H-7MTC reduced the production of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6. We also found that 4H-7MTC strongly exerted its anti-inflammatory actions by downregulating nuclear factor kappa B (NF-κB) activation by suppressing inhibitor of nuclear factor kappa B alpha (IκBα) degradation in macrophages. Moreover, 4H-7MTC decreased phosphorylation of extracellular signal-regulated kinase (ERK1/2) and c-Jun N-terminal kinase/stress-activated protein kinase (JNK), but not that of p38 MAPK. These results suggest that 4H-7MTC may be a good candidate for the treatment or prevention of inflammatory diseases such as dermatitis, psoriasis, and arthritis. Ultimately, this is the first report describing the effective anti-inflammatory activity of 4H-7MTC.

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Induction of Melanogenesis by Fosfomycin in B16F10 Cells Through the Upregulation of P-JNK and P-p38 Signaling Pathways

2020

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Fosfomycin disodium salt (FDS), which is a water-soluble extract, is a bactericidal drug used to inhibit the synthesis of cells. Moreover, it has been found to be effective in the treatment of urinary tract infections. The present study was conducted to investigate the melanogenesis-stimulating effect of FDS in B16F10 cells. Several experiments were performed on B16F10 cells: the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, the melanin content assay, the cellular tyrosinase activity assay, and Western blotting. FDS upregulated the activity of tyrosinase in a dose-dependent manner at a wide concentration range of 0–1 mg/mL, which showed no cytotoxicity. It also increased the melanin content and the activity of the microphthalmia-associated transcription factor (MITF), tyrosinase related protein 1 (TRP-1), and tyrosinase related protein 2 (TRP-2) enzymes in a dose-dependent manner. Western blotting results showed that FDS clearly upregulated the phosphorylation of c-Jun N-terminal kinases (JNK) and p38 pathways. These data are clear evidence of the melanogenesis-inducing effect of FDS in B16F10 murine melanoma cells.

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Anti-melanogenic Activity of Auraptene via ERK-mediated MITF Downregulation

Cited by 4 publications

References 23 publications

Ligularia fischeri ethanol extract: An inhibitor of alpha‐melanocyte‐stimulating hormone‐stimulated melanogenesis in B16F10 melanoma cells

Ligularia fischeri ethanol extract: An inhibitor of alpha‐melanocyte‐stimulating hormone‐stimulated melanogenesis in B16F10 melanoma cells

4-Hydroxy-7-Methoxycoumarin Inhibits Inflammation in LPS-activated RAW264.7 Macrophages by Suppressing NF-κB and MAPK Activation

Induction of Melanogenesis by Fosfomycin in B16F10 Cells Through the Upregulation of P-JNK and P-p38 Signaling Pathways

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