Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A

Oda, Ryo; Suardita, Ketut; Fujimoto, Katsumi; Pan, Hong; Yan, Weiqun; Shimazu, Atsushi; Shintani, Hideaki; Kato, Yukio

doi:10.1242/jcs.00393

Cited by 16 publications

(10 citation statements)

References 42 publications

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“…However, ConA and PNA exposure increased the size of the nodules and proteoglycan concentration. Earlier research found that ConA induced resting chondrocytes to convert to the hypertrophic phase [28] and produce proteoglycan [7]. Our data showed that PNA had the same effects in our mesenchymal cell cultures.…”

Section: Discussionsupporting

confidence: 84%

“…ConA induced the fibroblasts to form spherical cells with features characteristic of condrocytes, which were able to produce proteoglycans [7]. Some lectins induced an increase in the adhesion capacity of mesenchymal stem cells, chondrocytes and osteoblasts among other types of cell [24].…”

Section: Discussionmentioning

confidence: 99%

“…The distribution patterns of cell surface glycoconjugates are highly dynamic and related with structural modifications [5]. The uptake of several lectins such as concanavalin A (ConA), WGA and Ricinus communis agglutinin (RCA) by undifferentiated mesenchymal cells has been reported [6], and chondrocytes were also found to internalize membranebound ConA [7] and WGA [8].…”

Section: Introductionmentioning

confidence: 99%

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Lectins influence chondrogenesis and osteogenesis in limb bud mesenchymal cells

2011

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The role of cell surface glycoproteins in cell behavior can be characterized by their interactions with plant lectins. This study was designed to identify the effects of lectins on chondrogenesis and osteogenesis in limb bud mesenchymal cells in vitro. Limb bud mesenchymal cells from mouse embryos were cultured in high-density micromass culture. Wheat germ agglutinin (WGA), concanavalin A (ConA), peanut agglutinin (PNA), Dolichos biflorus agglutinin (DBA) and Ricinus communis agglutinin (RCA) were added separately to the culture media. Cells were cultured for 5 or 9 days, and cell viability was assayed by neutral red on day 5. The micromasses were stained with alcian blue, alizarin red S and Von Kossa stains, and alkaline phosphatase assays were also done. Dolichos biflorus agglutinin induced an increase in chondrogenesis, calcium precipitation and proteoglycan production. ConA and PNA did not affect chondrocyte differentiation but induced chondrocytes to produce more proteoglycan. Wheat germ agglutinin reduced chondrification and ossification but induced mesenchymal cells to store lipid droplets. Ricinus communis agglutinin 1 was toxic and significantly reduced cell survival. In conclusion, DBA was the most effective inducer of ossification and chondrification. Wheat germ agglutinin induced adipogenesis instead. These assays showed that lectins play important roles in limb bud development.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lectins influence chondrogenesis and osteogenesis in limb bud mesenchymal cells

2011

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“…For example, the disruption of the cytoskeletal structure of chondrocytes by staurosporine has been reported to induce morphological changes and enhance type II collagen expression [6]. The binding of concanavalin A to membrane-bound transferrin-like protein (MTf) on C3H10T1/2 cells, which is a murine cell line of mesenchymal stem cells, was reported to cause a cell-shape change from fibroblastic to spherical together with increases in the expression levels of aggrecan and type II collagen genes [7]. These reports suggested that the function of chondrocytes is correlated with their morphology.…”

Section: Analysis Of Two-dimensional (2d) Cell Morphologymentioning

confidence: 99%

Noninvasive quality estimation of adherent mammalian cells for transplantation

Takagi

2010

Biotechnol Bioproc E

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The noninvasive quality estimation of adherent mammalian cells for transplantation is reviewed. The quality and heterogeneity of cells should be estimated before transplantation because cultured cells are not homogeneous but heterogeneous. The estimation of cell quality should be performed noninvasively because most protocols of regenerative medicine are autologous cell system. The differentiation level and contamination of other cell lineage could be estimated by two-dimensional cell morphology analysis and tracking using a conventional phase contrast microscope. The noninvasive determination of the laser phase shift of a cell using a phase-shifting laser microscope, which might be more noninvasive, and more useful than the atomic force microscope and digital holographic microscope, was carried out to determine the three-dimensional cell morphology, and the estimation of the cell cycle phase of each adhesive cell and the mean proliferation activity of a cell population. Chemical analysis of the culture supernatant by conventional analytical methods such as ELISA was also useful to estimate the differentiation level of a cell population. Chemical analysis of cell membrane and intracellular components using a probe beam, an infrared beam, and Raman spectroscopy was useful for diagnosing the viability, apoptosis, and differentiation of each adhesive cell. © KSBB hÉóïçêÇëW=ÅÉää=éêçÅÉëëáåÖI=åçåáåî~ëáîÉI=èì~äáíó=ÅçåíêçäI=ãáÅêçëÅçéÉI=ä~ëÉêI=o~ã~å= = = = = CELL PROCESSING ENGINEERING AND CELL QUALITY ESTIMATIONCultivation engineering was established by many researchers aiming at the industrialization of the production of useful substances such as amino acids, antibiotics, and nucleic acids with the use of microbes. The research included medium design, reactor design, flask design, oxygen transfer theory, sensor design, modeling of microbial reactions, and optimization of microbial processes. Cultivation engineering for animal cells was developed from 1980 in order to industrialize the production of animal cell products such as interferon, interleukins, cytokines, tissue plasminogen activator, and therapeutic antibodies, which included the development of serum-free media, new aeration systems without sparging, and the designs of micro carriers for adhesion-dependent cells, reactors, and impellers design. The widespread use and industrialization of regenerative medicine have been highly

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“…The binding of concanavalin A to membrane-bound transferrin-like protein (MTf) on C3H10T1/2 cells, which is a murine cell line of MSCs, was reported to cause a cell-shape change from fibroblastic to spherical together with increases in the expression levels of aggrecan and type II collagen genes (Oda et al 2003). These reports suggested the notion that the function of chondrocytes is correlated with their morphology.…”

Section: Introductionmentioning

confidence: 99%

Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes

et al. 2008

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A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 lm 2 and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast-like to polygonal and the percentage of the large polygonal cells increased almost in proportion to aggrecan mRNA expression level during the differentiation culture of mesenchymal stem cells (MSCs) to chondrocytes. Approximately 80% of the large polygonal cells were negative for MSC marker (CD90, CD166) expression and the aggrecan mRNA expression level of the large polygonal cells was markedly higher than that of cells with other morphologies.

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Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A

Cited by 16 publications

References 42 publications

Lectins influence chondrogenesis and osteogenesis in limb bud mesenchymal cells

Lectins influence chondrogenesis and osteogenesis in limb bud mesenchymal cells

Noninvasive quality estimation of adherent mammalian cells for transplantation

Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes

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