Anti-MHC immunity detected prior to intentional alloimmunization

Abstract

Cell fusion was performed between spleen cells from young BALB/cBy (H-2d) mice which have never been immunized and SP2/0 mouse plasmacytoma cells. A monoclonal H-2 specific cytotoxic IgM antibody was obtained (By-1) which detected a new public biregional H-2 specificity, H-2.m210. The mcAb By-1 reacted strongly with H-2Kd, Dd, and H-2s antigens, gave weak cross-reactions with H-2Kk, Dq, H-2r, and H-2v antigens and was negative with H-2b, H-2f, H-2p, and H-2Ld antigens. A polymorphic reaction pattern was also observed on a panel of lymphocytes from B10.W strains. The intriguing finding on this reaction pattern was the reactivity on H-2d cells, including the syngeneic BALB/cBy and truly autologous cells. As shown by capping and immunoprecipitation experiments on H-2d cells and by studies on H-2d-transfected mouse L cells, the target molecules for McAb By-1 were H-2Kd and H-2Dd molecules. The BALB/cBy mouse, from whose spleen cells the McAb By-1 was obtained, survived after the fusion experiment, and serum was examined for the presence of cytotoxic H-2-specific antibodies during the rest of its life. At the time of the fusion, no autoreactive serum antibodies were found, but about 4 months later, we found in the serum of this mouse autoreactive H-2-specific cytotoxic IgM antibodies. The serum antibodies followed the same reaction pattern as that of the McAb By-1. As far as we know, this is the first report of autoreactive H-2-specific antibodies in serum of a mouse which has never been immunized and of the first natural autoreactive H-2-specific monoclonal antibody.

Induction of H‐2‐specific antibodies by injections of syngeneic Sendai virus‐coated cells

“…Furthermore, MHC-specific antibodies have been found in nonimmunized mice or in mice injected with syngeneic cells [18][19][20]. Infection, polyclonal stimulation [ 171 or modification of MHC antigens by viruses [16,211 have been suggested for explanation of the origin of these MHC-specific antibodies detected without prior alloimmunization.…”

Anti‐mhc Immunity Detected Prior to Intentional Alloimmunization

“…(1) With more data, information can be obtained about the B cell allo-repertoire prior to intential alloimmunization, about the postnatal development of a change in this repertoire and about its functional significance (Wolosin & Greenberg, 1981;Ivanyi et af., 1982;Lynes et al, 1984;Cerny-Provaznik et al, 1985a,c,d;Cerny-Provaznik & Ivanyi, 1985;Longenecker & Mosmann, 1980;Neu et al, 1984;Gunther et af., 1983;Kloting & Stark, 1985;Tongio el al., 1985).…”

“…Naturally occurring H-2-specific antibodies can be detected rather frequently in sera of non-alloimmunized mice by sufficiently sensitive techniques (Cerny-Provaznik et al, 1985a;Cerny-Provaznik & Ivanyi, 1985). In this report, we summarize our experiences with the preparation of monoclonal anti-H-2 antibodies obtained from hybridization experiments from non-alloimmunized mice.…”