Anti-microbial Action against Verotoxigenic<i>Escherichia coli</i>O157:H7 of Nitric Oxide Derived from Sodium Nitrite

Yoshikawa, Hiroshi; Suzuki, Takehito; Hisamatsu, Shin; Kato, Yukio; Sakata, Ryoichi; Nagata, Yasuo; Yoshimura, Tetsuhiko

doi:10.1271/bbb.68.1027

Cited by 23 publications

(10 citation statements)

References 17 publications

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“…In enteropathogenic strains of Escherichia coli, which are predominantly responsible for infantile diarrhoea, outer membrane proteins activate epithelial cell iNOS and induce NO production through activation of NF-κB and MAP kinases (Malladi et al, 2004). Furthermore, sodium nitrite, which is used as an additive in cured meats, exerts antimicrobial and thus preservative effects through generation of NO, which is toxic to verotoxigenic strains of E. coli (Morita et al, 2004). Thus, NO represents an important antimicrobial agent, of special significance to pathogens.…”

Section: Introductionmentioning

confidence: 99%

The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: Links with swarming motility

Stevanin

Read

Poole

2007

Gene

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Section: Introductionmentioning

confidence: 99%

The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: Links with swarming motility

Stevanin

Read

Poole

2007

Gene

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“…NO potentiates hydrogen peroxide (H 2 O 2 )-induced killing of E. coli in part through the generation of ONOO Ϫ (3,40). Nitrite exerts antimicrobial effects through generation of NO, which is toxic to STEC (35). Furthermore, NO suppresses Stx2 production by STEC through inhibition of stx 2 mRNA expression (51).…”

mentioning

confidence: 99%

Subtilase Cytotoxin Enhances Escherichia coli Survival in Macrophages by Suppression of Nitric Oxide Production through the Inhibition of NF-κB Activation

et al. 2012

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e Subtilase cytotoxin (SubAB), which is produced by certain strains of Shiga-toxigenic Escherichia coli (STEC), cleaves an endoplasmic reticulum (ER) chaperone, BiP/Grp78, leading to induction of ER stress and caspase-dependent apoptosis. SubAB alters the innate immune response. SubAB pretreatment of macrophages inhibited lipopolysaccharide (LPS)-induced production of both monocyte chemoattractant protein 1 (MCP-1) and tumor necrosis factor ␣ (TNF-␣). We investigated here the mechanism by which SubAB inhibits nitric oxide (NO) production by mouse macrophages. SubAB suppressed LPS-induced NO production through inhibition of inducible NO synthase (iNOS) mRNA and protein expression. Further, SubAB inhibited LPS-induced IB-␣ phosphorylation and nuclear localization of the nuclear factor-B (NF-B) p65/p50 heterodimer. Reporter gene and chromatin immunoprecipitation (ChIP) assays revealed that SubAB reduced LPS-induced NF-B p65/p50 heterodimer binding to an NF-B binding site on the iNOS promoter. In contrast to the native toxin, a catalytically inactivated SubAB mutant slightly enhanced LPS-induced iNOS expression and binding of NF-B subunits to the iNOS promoter. The SubAB effect on LPS-induced iNOS expression was significantly reduced in macrophages from NF-B1 (p50)-deficient mice, which lacked a DNA-binding subunit of the p65/p50 heterodimer, suggesting that p50 was involved in SubAB-mediated inhibition of iNOS expression. Treatment of macrophages with an NOS inhibitor or expression of SubAB by E. coli increased E. coli survival in macrophages, suggesting that NO generated by macrophages resulted in efficient killing of the bacteria and SubAB contributed to E. coli survival in macrophages. Thus, we hypothesize that SubAB might represent a novel bacterial strategy to circumvent host defense during STEC infection.

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“…Benedict (1980) and Tompkin (2005) found that nitrite has a bacteriostatic effect and in combination with salt inhibits food spoilage bacteria. Nitrous acid, which is in chemical equilibrium with nitrite in acid medium, has been shown to be reactive with several bacterial protein groups associated with cellular energy metabolism (Morita et al 2004;Woods et al 1981) or with membrane permeability (Buchman and Hansen 1987;Cammack et al 1999).…”

Section: Microbial Countsmentioning

confidence: 99%

Quality Attributes and Shelf Life of High-Pressure Preserved Beef as Affected by Pre-treatment Conditions

Giménez

Graiver

Califano

et al. 2017

Food Bioprocess Technol

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This study analyzed the effects of high hydrostatic pressure (HHP) and composition of the pre-treatment immersion step, on quality attributes (color and lipid oxidation) and shelf life based on microbial counts of a beef product, during cold storage at 0°C. Meat slices were immersed in a preservative solution containing sodium nitrite, ascorbic acid, and two different concentrations of NaCl (30 and 60 g/L); HHP of 400 and 600 MPa were applied. Results were compared with those of an untreated beef control. Color parameters of the HHP-treated beef were visually acceptable (a* > 14) in all tested cases, although they were affected by NaCl concentration and the applied pressure. HHP increased TBARS index, observing higher values at 600 than at 400 MPa; samples immersed in the solution containing 30 g/L NaCl presented higher TBARS values. However, in all cases, they remained below the detection limit of rancid meat products (<1 mg MDA/kg). Beef samples immersed in the solution with the highest concentration of NaCl (60 g/L) and subjected to 400 or 600 MPa maintained their microbial stability over 5 and 6 weeks, respectively, at 0°C; these shelf life values were higher than those observed in the samples treated with 30 g/ L NaCl.

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Anti-microbial Action against VerotoxigenicEscherichia coliO157:H7 of Nitric Oxide Derived from Sodium Nitrite

Cited by 23 publications

References 17 publications

The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: Links with swarming motility

The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: Links with swarming motility

Subtilase Cytotoxin Enhances Escherichia coli Survival in Macrophages by Suppression of Nitric Oxide Production through the Inhibition of NF-κB Activation

Quality Attributes and Shelf Life of High-Pressure Preserved Beef as Affected by Pre-treatment Conditions

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