Anti-mitogenic action of opioid peptides on epidermal growth factor-stimulated uterine cells

Környei, J.; Oszter, Angéla; Kovács, Kálmán; Vértes, Zsuzsanna; Komlósi, Katalin; Göcze, Péter M.; Vértes, Marietta

doi:10.1016/s0014-2999(01)00790-7

Cited by 2 publications

(19 citation statements)

References 57 publications

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“…Samples were placed in 10 ml HBSS containing 0.1% collagenase and 0.005% DNaseI and incubated at 37 8 for 30 min, and the dispersed cells were digested with 0.01% trypsin and 0.005% DNaseI at 37 8 for 5 min. Pellets were washed three times with HBSS and cultured in enriched DMEM (high glucose) containing 10% fetal bovine serum, 2% antibioticantimycotic solution and 0.004% kanamycin (Kornyei et al 2001). All chemicals were purchased from SigmaAldrich.…”

Section: Primary Cultures Of Rat Endometrial Cellsmentioning

confidence: 99%

Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Pohóczky

Kun

Szalontai

et al. 2015

Journal of Molecular Endocrinology

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Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors expressed predominantly in sensory nerves are activated by inflammatory stimuli and mediate inflammation and pain. Although they have been shown in the human endometrium, their regulation and function are unknown. Therefore, we investigated their estrogen-and progesterone-dependent alterations in the rat endometrium in comparison with the estrogenregulated inflammatory cytokine macrophage migration inhibitory factor (MIF). Four-week-old (sexually immature) and four-month-old (sexually mature) female rats were treated with the non-selective estrogen receptor (ER) agonist diethylstilboestrol (DES), progesterone and their combination, or ovariectomized. RT-PCR and immunohistochemistry were performed to determine mRNA and protein expression levels respectively. Channel function was investigated with ratiometric [Ca 2C ] i measurement in cultured primary rat endometrial cells. Both TRP receptors and MIF were detected in the endometrium at mRNA and protein levels, and their localizations were similar. Immunostaining was observed in the immature epithelium, while stromal, glandular and epithelial positivity were observed in adults. Functionally active TRP receptor proteins were shown in endometrial cells by activation-induced calcium influx. In adults, Trpa1 and Trpv1 mRNA levels were significantly up-regulated after DES treatment. TRPA1 increased after every treatment, but TRPV1 remained unchanged following the combined treatment and ovariectomy. In immature rats, DES treatment resulted in increased mRNA expression of both channels and elevated TRPV1 immunopositivity. MIF expression changed in parallel with TRPA1/TRPV1 in most cases. DES up-regulated Trpa1, Trpv1 and Mif mRNA levels in endometrial cell cultures, but 17b-oestradiol having ERa-selective potency increased only the expression of Trpv1. We provide the first evidence for TRPA1/TRPV1 expression and their estrogen-induced up-regulation in the rat endometrium in correlation with the MIF. Journal of Molecular EndocrinologyResearch K POHÓ CZKY and others TRPV1 and TRPA1 in the rat endometrium 56:2 135-149

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Section: Primary Cultures Of Rat Endometrial Cellsmentioning

confidence: 99%

Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Pohóczky

Kun

Szalontai

et al. 2015

Journal of Molecular Endocrinology

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“…The number of rats varied from 15 to 46 depending on the age of the animals and the size of the experiment. To compare with previous studies (Környei et al . 1997, 2001), some experiments were performed with rats from the CFY strain as well.…”

Section: Methodsmentioning

confidence: 96%

“…Rat uterine cells were dispersed by subsequent limited enzymatic digestions with trypsin, then collagenase as described previously (Környei et al . 1997, 2001) for 60‐day‐old animals.…”

Section: Methodsmentioning

confidence: 99%

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Developmental changes in the inhibition of cultured rat uterine cell proliferation by opioid peptides

Vértes

et al. 2003

Cell Proliferation

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Opioid peptides are negative regulators of cell proliferation in several organs including the uterus. In the present study, the ontogeny of the direct inhibitory action of opioid peptides on the proliferation of cultured rat uterine cells was investigated. Uteri of 7, 14, 21, 28, 35 and 60-day-old rats were removed in a sterile way. Tissue blocks were dispersed by limited digestions with trypsin and collagenase. Cells were cultured in enriched Dulbecco's modified Eagle's medium (DMEM). Treatments were present during the entire culture period. Cell densities of the monolayers were determined by counting the cells following trypsinization and trypan blue exclusion. Rat uterine mixed cell cultures grew to confluence within 10 days. The average population doubling time gradually increased with the age of animals. Epidermal growth factor (EGF) increased cell densities of cultures from all age groups. The oestradiol (E2)-responsiveness appeared at 21 days of age. The effect of [D-Met2-Pro5]-enkephalinamide (ENK) was biphasic. ENK and [Met5]-enkephalin (OGF) decreased cell densities of both unstimulated and EGF-stimulated cultures from 7-day-old rats to the same extent. ENK failed to act in 14-day-old animals. From 21 days of age on, the E2- or EGF-stimulated proliferation was inhibited only by ENK and DAMGO, while 30 nm DPDPE, Dynorhin-A, OGF, [Leu5]-enkephalin, beta-endorphin, and morphiceptin were ineffective. The half-inhibitory concentration of ENK was 0.3 nm. The effects of ENK were prevented by concomitant treatment with naloxone. Our novel data demonstrate two different phases of the inhibitory action of opioid peptides on rat uterine cell proliferation during ontogeny with an insensitive interval in between.

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Anti-mitogenic action of opioid peptides on epidermal growth factor-stimulated uterine cells

Cited by 2 publications

References 57 publications

Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Developmental changes in the inhibition of cultured rat uterine cell proliferation by opioid peptides

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