Anti-Müllerian hormone and Oestradiol as markers of future reproductive success in juvenile gilts

Serum Concentrations of AMH and E2 and Ovarian and Uterine Traits in Gilts

“…After thawing, serum samples were diluted 1:2 in PBS, and AMH was quantified using a competitive inhibition ELISA kit (CEA228Po: Cloud-Clone Corp, TX, USA) using monoclonal antibodies specific for porcine AMH. In-house validation of this kit was performed as described previously [18]. The minimum detectable dose for the assay kit was 135.8 pg/mL.…”

“…Circulating oestradiol (E2) is another candidate hormone that could be indicative of gilts with high reproductive potential. We previously demonstrated that circulating E2 levels in juvenile gilts aged 60, 80 or 100 days of age were positively associated with the probability of stillbirth and negatively associated with the number of piglets born alive in parities one to three [18]. This period was chosen because this is a critical window for ovarian development in pigs [19] and the levels at 80 days of age were found to be the most useful measure.…”

Evaluation of Ovarian Reserve by Using AMH Test before Collection the Oocytes for ICSI Technique

“…The study showed significant increases in the level of AMH (4.02 ± 0.17) of group I (˂ 4 years old) comparative with (2.22 ± 0.54) to the group II (> 4 years old) at the (<0.01), table (1), and this result relation with the number of oocytes obtained from ovarian samples of animals after slaughtering, it was found that the number and quality of oocytes increased in the group I (29 oocytes from 15 ovarian samples , compared with group II (16 oocytes from15ovarian samples , table (2). This result explains the decrease in the ovarian reservoir of follicles with increase the age in the doe as a result of the decrease in the level of AMH in the serum, which is considered an indicator for knowing the ovarian reservoir from the follicles and determining the productive age of the ovaries related to the association of AMH with age or parity seems contradictory in cattle [18] and compatible with [17] reported there were greater conception rates in primiparous than multiparous heifers and similar fertility. While with the study of [19] there was not any significant correlation between AMH and parity, in other studies concentrations of AMH in second and third lactation cattle were greater than in cows with their first and fourth or more lactations [5] .…”

“…The AMH test is considered one of the important tests that must be taken before oocyte collection to reduce the effort and costs involved in conducting any assisted reproduction technique that requires collecting the oocytes from the donor. The AMH concentration is closely correlated with the size of the ovarian primordial follicle reserves [3] and [18] . A highly significant (HS) difference between the two groups for the AMH at P-value <0.01…”

Relationship among ovarian follicular status, developmental competence of oocytes, and anti‐Müllerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts

“…Therefore, AMH is also considered a useful endocrine marker to assess reproductive performance in the fields of obstetrics and gynecology (van Rooij et al., , , ). In pigs, it has been demonstrated that AMH appears for the first time during follicular development in the fusiform granulosa cells of recruited primordial follicles and continues to be present in granulosa cells up to the antral stage (Almeida et al., ), indicating that plasma AMH levels may be an effective marker of reproductive performance, including litter size (Steel, Athorn, & Grupen, ).…”