Anti-Müllerian hormone as a diagnostic tool for ovarian remnant syndrome in bitches

Abstract

Ovariohysterectomy is a surgical procedure performed for sterilization. If part or all of an ovary remains after the surgical procedure, ovarian remnant syndrome (ORS) occurs. After revascularization of the remaining ovarian tissue, the sexual cycle of bitches and queens continues. The aim of this study was to investigate the usefulness of serum anti-Müllerian hormone (AMH) measurement for the diagnosis of ORS in bitches. Forty-six female dogs were divided into four groups: the prepubertal, unspayed, spayed, and ORS groups. The serum AMH, progesterone, and estradiol concentrations of the bitches in all groups were measured by enzyme-linked immunosorbent assay. The stage in the sexual cycle of each bitch was determined based on vaginal cytology findings and serum estradiol and progesterone levels. The mean serum AMH concentration of the unspayed bitches (4.26 ± 0.82 ng/ml) was similar to that of the bitches with ORS (4.40 ± 1.09 ng/ml). However, the difference between the mean serum AMH level of the bitches with ORS and the spayed bitches (0.28 ± 0.09 ng/ml) was significant. In conclusion, the present study has demonstrated that the serum AMH level is substantially lower in spayed than in both intact bitches and bitches with ORS. According to this result, measurement of the serum AMH level is a useful tool for diagnosis of ORS in bitches.

Evcil Kedilerde Yaş İlişkili Anti-Müllerian Hormon Değişimlerinin Değerlendirilmesi

“…To study cyclic variations of serum AMH levels in cats, sampling from higher number of animals are required for demonstration of AMH dynamics. Although, serum AMH concentrations are decreased after OHE in bitches [8] when compared to our results, these levels were non-detectable ten days after OHE (<0.08 ng/mL). This indicates that AMH might be a better indicator for detecting the presence of ovaries in cats than dogs.…”

“…Recently, AMH was suggested as an important indicator for the detection of presence of the ovaries in animals with unknown history and diagnosis of ORS cases as well in veterinary medicine. In a previous study [7] , serum AMH levels were decreased after OHE in cat and also in bitch [6,8] . In the present study, similar to Axner et al [7] and others [6,8] , mean serum AMH levels (3.15 ng/mL) before OHE was dramatically decreased three days after OHE and dropped down to non-detectable concentrations at ten days after OHE in all cats (below 0.08 ng/mL).…”

“…In females, AMH is produced only in ovaries by the granulosa cells of growing follicles. Therefore the measurement of AMH levels plays an important role for the diagnosis the presence or absence of the ovaries which have increasingly gain popularity [6][7][8] .…”

“…In recent years, the practicability of serum AMH levels to diagnose the presence of the ovaries in female cats is discussed in some studies [6][7][8] but additional studies are required to establish the references ranges of serum AMH levels in domestic cat.…”

Anti‐Muellerian hormone concentration in bitches with histopathologically diagnosed ovarian tumours and cysts

“…(), who examined AMH for determining the reproductive status and the AMH values of bitches during the period from late anoestrus to pro‐oestrus, respectively. Other authors used a canine‐specific ELISA tests and measured much higher AMH values in bitches (Hollinshead, Walker, & Hanlon, ; Turna Yilmaz et al., ). Bitches without pathological findings of the ovaries in the present study had AMH values from 0.12 to 0.99 ng/ml, which is in the range of intact bitches older than 6 months described by Place et al.…”