Anti-mycobacterial natural products from the Canadian medicinal plant Juniperus communis

Carpenter, Caitlyn D.; O'Neill, Taryn; Picot, Nadia; Johnson, John A.; Robichaud, Gilles A.; Webster, Duncan; Gray, Christopher A.

doi:10.1016/j.jep.2012.07.035

Cited by 69 publications

(30 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). The MRA was also successfully employed in the bioassay-guided fractionation of the anti-mycobacterial constituents of three of these plant extracts (A. nudicaulis (Li et al, 2012), H. maximum (O'Neill et al, 2013) and J. communis (Carpenter et al, 2012)).…”

Section: Resultsmentioning

confidence: 99%

“…Natural products have a proven history as drug leads (Newman and Cragg, 2012) and have high potential to become or inspire new anti-mycobacterial agents (Copp, 2003;Copp and Pearce, 2007;Garcia et al, 2012;Salomon and Schmidt, 2012). An example is the investigation of traditionally used Canadian medicinal plants and their endophytic fungi for anti-mycobacterial natural products (Webster et al, 2010;Carpenter et al, 2012;Li et al, 2012;Gray et al, 2013;O'Neill et al, 2013). For this study, a fluorometric bioassay based on the reduction of resazurin was envisaged both for initial screening and bioassay-guided fractionation of plant and fungal extracts, because it has been reported to be a simple, accurate and reliable bioassay for evaluation of mycobacterial growth (Primm and Franzblau, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimisation of the Microplate Resazurin Assay for Screening and Bioassay‐guided Fractionation of Phytochemical Extracts against Mycobacterium tuberculosis

O'Neill

Colquhoun

et al. 2014

Phytochemical Analysis

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimisation of the Microplate Resazurin Assay for Screening and Bioassay‐guided Fractionation of Phytochemical Extracts against Mycobacterium tuberculosis

O'Neill

Colquhoun

et al. 2014

Phytochemical Analysis

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some alkaloids, flavonoids, glycosides, tannins, phenolic compounds, and steroids from JCo berries and leaves can be extracted using chloroform and methanol [23]. The pharmacological activities of these chemical constituents include anti-inflammatory [24,25], antifungal [26], analgesic [27], hepatoprotective [23], antimicrobial, anti-mycobacterial [28,29], and neuroprotective [22] effects. In a recent study, Benzina et al demonstrated that deoxypodophyllotoxin extracted from JCo induced apoptosis in breast cancer cells [30].…”

Section: Introductionmentioning

confidence: 99%

Juniperus Communis Extract Exerts Antitumor Effects in Human Glioblastomas Through Blood-Brain Barrier

Цай

Tsai

Chang

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Herbal materials derived from Juniperus communis (JCo) possess anticancer activity. In this study, we evaluated the efficacy of a JCo berry extract in suppressing glioblastoma growth. Methods: The effects of JCo extract on the viability of normal and glioma cell lines was analyzed using a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The synergistic therapeutic effect of JCo extract and temozolomide (TMZ) on glioma cells was examined by MTT analysis. Flow cytometry analysis, the terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) test, and western blotting were performed to identify the apoptotic pathway. To determine the in vivo efficacy of the JCo extract, rats were injected with 5 × 10⁴ rat glioma RG2 cells in the back skin and brain hemisphere and then received a subcutaneous injection in the back skin that contained either JCo extract or vehicle. Finally, blood and histologic examinations were performed to evaluate JCo toxicity. Results: The IC₅₀ values of JCo extract were 57–69 µg/mL and 49–67 µg/mL in the glioblastoma cell lines after 24 and 48 h, respectively. However, in non-tumor cell lines, the respective IC₅₀ values of JCo extract were 76–105 µg/mL and 77–108 µg/mL. The JCo extract had a stronger cytotoxicity and a larger range of IC₅₀ values in glioma than in normal cells as compared to those effects caused by temozolomide (TMZ). In addition, the results of flow cytometry analysis, TUNEL test, and western blotting revealed that the JCo extract induced glioma cell cycle arrest through intrinsic and extrinsic apoptotic pathways. In the in vivo studies, a significant reduction of tumor size in JCo-treated rats, as measured by animal MRI, demonstrated that the JCo extract effectively inhibited glioma cell growth and successfully penetrated the blood-brain barrier. The immunohistochemical (IHC) staining detected positive signals of PCNA, VEGFR-1, and VEGFR -2 in 44.49%, 5.88%, and 5.85% of JCo-treated glioma cells, respectively. However, positive signals of PCNA, VEGFR-1, and VEGFR-2 were detected in 73.08%, 9.67%, and 11.70% of vehicle-treated glioma cells, respectively. The IHC examination of PCNA and VEGFR-1 and -2 indicated that JCo extract significantly decreased the degree of neovascularization. However, no significant differences in serum levels of blood cell count and hepatic enzymes, renal function index, and the histologic appearance of vital organs were detected between the JCo and vehicle-treated rats. Conclusion: The JCo extract penetrated the blood-brain barrier and significantly induced glioma cell apoptosis by reducing neovascularization via suppression of the PI3K/AKT/mTOR pathway. Furthermore, JCo extract was less cytotoxic to non-neoplastic vital organs than TMZ.

show abstract

“…A salt solution was made next by adding malachite green 2% solution. After adding the salt solution and malachite solution to the eggs, they were placed on a hot plate so they could become mixed [10].…”

Section: Preparing Levin-stein Johnson Vitro Containing Capparis Spinmentioning

confidence: 99%

“…This Capparaceae have 30 genera and 650 species, and they grow mainly in warm and temperate areas and grow like shrubs [10]. Their bushes are crawling with long life and downy, and they have ramified branches with 1-1.5 m length.…”

Section: Introductionmentioning

confidence: 99%

Anti-Mycobacterial Activity of Capparis Spinosa L. Extract Against Clinical Isolates of Mycobacterium Tuberculosis

Ehsanifar

Kazemipoor

Fouladi

2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: Tuberculosis (TB) has been one of the biggest global health issues for decades. Emerging forms of the disease; however, after many years since the discovery of the last effective treatment for this disease, the emerging forms of it have made scientists worried about the disease. Most herbs have anti-microbial properties; however, there was no research on the impact of fruit Capparis spinosa on TB. This study was conducted to evaluate the anti-microbial effects of bacterial C. spinosa. Methods:In this study, methanolic extract at maximum efficiency to maintain existing substances was prepared by the freeze dryer device and to determine the minimum inhibitory concentration of bacteria's growth. The proportional method was used on 7 clinical samples and one reference sample H37RV with its six concentrations (80-50-25-10-1-0/2 mg/ml). In addition, to determine the effects of plant extracts in combination with rifampin, all samples were tested by the supplement combination or cover combination of these two materials. All tests were performed twice. The methanol extract of the plant at the concentration of 25 mg/mL in all samples has inhibitory effect on the growth. Results:In study, clinical samples with a lethal dose of RIFAMPIN, it was shown that none of the samples have drug resistance. The results have revealed that the methanol extract has an anti-mycobacterial effect. Conclusion:The results of this study indicate that Capparis spinosa possess considerable anti-mycobacterial properties in the laboratory. These results can be used as a background for further future studies on this species.

show abstract

Anti-mycobacterial natural products from the Canadian medicinal plant Juniperus communis

Cited by 69 publications

References 34 publications

Optimisation of the Microplate Resazurin Assay for Screening and Bioassay‐guided Fractionation of Phytochemical Extracts against Mycobacterium tuberculosis

Optimisation of the Microplate Resazurin Assay for Screening and Bioassay‐guided Fractionation of Phytochemical Extracts against Mycobacterium tuberculosis

Juniperus Communis Extract Exerts Antitumor Effects in Human Glioblastomas Through Blood-Brain Barrier

Anti-Mycobacterial Activity of Capparis Spinosa L. Extract Against Clinical Isolates of Mycobacterium Tuberculosis

Contact Info

Product

Resources

About