Anti-Mycobacterium Tuberculosis Strain H37rv and Iron Chelation Activity of Sappan Wood Extract (Caesalpinia Sappan L.) in Vitro

Abstract

Objective: The objective of this study is to determine anti-Mycobacterium tuberculosis (MTB) strain H37Rv and iron chelation activities of sappan wood extract (SWE).Methods: The evaluation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) by proportion methods. Whereas the iron chelate in Lowenstein–Jensen (LJ) medium as the indicator of Mycobacterium growth and SWE effect.Results: The SWE has bacteriocidal to MTB of 10−3 and 10−5 dilutions in of all concentrations (250, 500, 750, 1000, 2000, 4000, 8000, and 16000 part per millions [ppm]) also bacteriostatic in concentration 50 and 100 ppm.Conclusion: The SWE at 100 ppm could inhibit 87% of the MTB in 10−3 and 10−5 dilutions, respectively, also to reduce to growth the colony of MTB, and has chelating effects of iron expression of LJ medium and MTB.

Antituberculosis Activity and Iron Chelation Ability of Brazilin Isolated from Caesalpinia Sappan L.

“…This result showed Sappan wood has iron chelating activity. The ability of brazilin compounds inhibits Mtb growth also confirmed by previous research, according to Safitri [27]. Sappan wood extract at a concentration of 100 ppm showed an inhibitory ability of MTB strain H37Rv growth at week six.…”

“…It is indicated that Mtb growth requires iron as a nutrient. Anti-tubercular properties of Brazilin are thought to be due to the iron chelation properties of Brazilin, which may be caused by the presence of multiple OH groups and the catechol structure as an iron-binding site [27].…”

“…In addition, several natural polyphenols have synergistic properties when combined with general chemotherapy [38]. Brazilin is a flavonoid; it has antimicrobial potency because the catechol group inhibits energy metabolism by binding the iron for Mtb energy metabolism [27]. Other phenolic compounds, such as kaempferol, hesperetin, and biochanin, can inhibit the growth of other bacteria such as Escherichia coli by inhibiting the metabolism of liposomes [39].…”

Spirocyclic derivatives as antioxidants: a review

“… 94 In Indonesia, its material has long been used in folk medicine to treat tuberculosis, diarrhea, dysentery, skin infections, anemia, detoxification, syphilis treatment, an antiseptic to stop bleeding, and pain due to blood circulation disorders. 95 …”

“…94 In Indonesia, its material has long been used in folk medicine to treat tuberculosis, diarrhea, dysentery, skin infections, anemia, detoxi-cation, syphilis treatment, an antiseptic to stop bleeding, and pain due to blood circulation disorders. 95 The investigation of the chemical components of C. sappan wood resulted in the isolation of several structural types of phenolic components, including xanthones, coumarins, chalcones, avones, homoisoavonoids, and brasilin. 96 Scien-tic studies validated most of C. sappan L.'s folkloric uses as an AO, including its antibacterial, anti-inammatory, antiphotoaging, hypoglycemic vasorelaxant, hepatoprotective, iron-chelating agent, and anti-acne activities.…”