Anti-neoplastic effect of β-hydroxyisovalerylshikonin on a human choriocarcinoma cell line

Abstract

Abstract.β-hydroxyisovalerylshikonin (β-HiVS), a compound isolated from the traditional asian medicinal herb Lithospermum radix, is an aTP non-competitive inhibitor of protein-tyrosine kinases such as v-Src and eGFr, and has been shown to induce apoptosis in several human tumor cell lines. We investigated the effect of β-HiVS in the choriocarcinoma cell line, BeWo. BeWo cells were treated with various concentrations of β-HiVS, and changes in cell growth, the cell cycle, apoptosis, and related parameters were examined. an MTT assay showed that BeWo cells were sensitive to the growth inhibitory effect of β-HiVS. cell cycle analysis indicated that exposure to β-HiVS decreased the proportion of cells in the S phase and increased the proportion in the G 0 /G 1 phases of the cell cycle. Induction of apoptosis was confirmed by annexin V staining of externalized phosphatidylserine and by the loss of mitochondrial transmembrane potential. This induction occurred in conjunction with the altered expression of genes related to cell growth, malignant phenotype, and apoptosis. These results suggest that β-HiVS may serve as a therapeutic agent for the treatment of choriocarcinoma.

β-hydroxyisovaleryl-shikonin induces human cervical cancer cell apoptosis via PI3K/AKT/mTOR signaling

“…When 10 −6 M β-HIVS was used to treat HL-60 cells for 3 h, typical apoptotic characteristics were observed in the cells, including morphological changes, nuclear fragmentation, DNA ladder formation and caspase-3 activation, which indicates that β-HIVS induced tumor cell apoptosis through a caspase-3-dependent mechanism. Previous studies ( 28 , 31 ) verified that β-HIVS exerted inhibitory and proapoptotic effects on endometrial cancer, chorionic carcinoma and ovarian cancer cells, with IC 50 values ranging between 10 −6 and 10 −8 M.…”

Beta-Hydroxyisovalerylshikonin Inhibits the Growth of U266 Multiple Myeloma Cells by Triggering the Mitochondrial Pathway

“…Beta-HIVS is an ATP non-competitive inhibitor of protein-tyrosine kinases and is capable of inducing apoptosis in various lines of human tumor cells which has been documented by several publications [9][10][11][12][13][14][15][16]. Hashimoto et al, 2002 found that beta-HIVS was able to induce cell death more efficiently in DMS114 and NCI-H522 lung cancer cells [6].…”

Τεχνολογία Δευτερογενών Μεταβολιτών

“…Μία άλλη οδός για την καταπολέμηση του καρκίνου είναι και η αναστολή της αγγειογένεσης. Η σικονίνη έχει μελετηθεί ως πιθανός αναστολέας της αγγειογένεσης (Hisa et al, 1998) (Bhatia et al, 2011;Takai et al, 2010;Xiong et al, 2009). Ανάλογη έρευνα μελέτησε την αντικαρκινική δράση αλκαννίνης και εστέρων της, που απομονώθηκαν από ρίζες του φυτού Alkanna cappadocica, έναντι δώδεκα ανθρώπινων καρκινικών σειρών με ιδιαίτερα ενθαρρυντικά αποτε-λέσματα (Sevimli-Gur et al, 2010).…”