Anti-Neospora caninum antibodies in beef cattle from the northern region of Paraná state, Brazil

Abstract

The presence of anti-Neospora caninum antibodies in beef cattle slaughtered in the northern region of the state of Paraná, Brazil, was evaluated. A total of 401 blood samples were collected; 281 samples from the municipality of Rolândia and 120 from the municipality of Borrazópolis, between April 2015 and November 2016. Of the total samples, 289 were from females and 112 from males, aged one and a half to eight years. Indirect fluorescent antibody test (IFAT) was performed, using a cut-off of 1:100. Variables were tabulated for statistical analyses (Fisher’s exact test and chi-square tests, p≤0.05). The analysis showed that of the 401 samples, 37 were positive for N. caninum, indicating a prevalence of 9.2 %, and observed titers were 1:100 (16), 1:200 (14), and 1:400 (7). The variables sex, age, and location did not differ statistically (p>0.05). Our results showed a sero-occurrence of N. caninum in cattle slaughtered in the northern region of the state of Paraná.

Detecção De Anticorpos Anti-Neospora Caninum Por Ensaio Imunoenzimático Em Ovinos Do Município De São Roque No Estado De São Paulo

“…Pouco são os relatos de neosporose clínica, entretanto sabe-se que alguns surtos de aborto e desordens reprodutivas em ovinos e bovinos devem estar relacionados aos testes positivos frente a Neospora caninum (CABRAL et al, 2009;ROSA et al, 2011;MACEDO et al, 2013;WARLETA et al, 2014;MACEDO et al, 2017;GUERRA et al, 2019;SANTOS et al, 2020) Testes sorológicos indiretos como o ELISA e testes diretos como o PCR, são prérequisitos para confirmação do diagnóstico do agente infeccioso (ASADPOUR et al, 2012;PINHEIRO et al, 2015). A associação dos testes direto e indireto pode ser necessária tendo em vista que animais, mesmo livres de infecção, podem permanecer soropositivos por aproximadamente 6 meses (CARDOSO et al, 2012).…”