2016
DOI: 10.1016/j.phymed.2016.05.002
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Anti-neuroinflammatory and neuroprotective effects of the Lindera neesiana fruit in vitro

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Cited by 31 publications
(40 citation statements)
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“…We purchased the C6 glioma cells from the Korean Cell Line Bank (Seoul, Korea), whereas the N2a cells were obtained from American Type Culture Collection (Manassas, VA, USA). All cell culture and maintenance procedure was done as described previously [38]. …”
Section: Methodsmentioning
confidence: 99%
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“…We purchased the C6 glioma cells from the Korean Cell Line Bank (Seoul, Korea), whereas the N2a cells were obtained from American Type Culture Collection (Manassas, VA, USA). All cell culture and maintenance procedure was done as described previously [38]. …”
Section: Methodsmentioning
confidence: 99%
“…3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was used to determine the cell viability of BV2 cells 24 h after the exposure with LPS in the presence or absence of different concentrations of Equol as described previously [38] with some modification. Supernatant or medium was removed from the treated plate and MTT solution was added in the cell and it was incubated for an hour.…”
Section: Methodsmentioning
confidence: 99%
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“…Acid Hydrolysis of 1b and 2 and Sugar Analysis Each compound (1b, 0.6 mg; 2, 1 mg) was refluxed with 1 N HCl (1 mL) at 90°C for 3 h. The hydrolysate was extracted with EtOAc, and the aqueous layer was neutralized using an Amberlite IRA-67 column to yield the sugar. The sugar was obtained from each H 2 …”
Section: Methodsmentioning
confidence: 99%
“…The released amount of nitrite ( NO2-), a soluble oxidation product of NO, was measured with a colorimetric assay using Griess reagent (1% sulfanilamide and 0.1% N -(1-naphthyl)-ethylenediamine dihydrochloride in 5% phosphoric acid (H 3 PO 4 )) (Subedi et al, 2016). BV-2 cells (5 × 10 4 cells/well, in a 96-well plate)were seeded overnight to attach the cells, then stimulated with either LPS/IFNγ (0.2 μg/ml and 0.2 ng/ml, respectively) in the presence or absence of PGG (25 to 200 μM) for 24 h. Control cells were treated with DMSO.…”
Section: Methodsmentioning
confidence: 99%