Anti-nociceptive and anti-inflammatory activities of (−)-α-bisabolol in rodents

Abstract

(-)-α-Bisabolol is an unsaturated, optically active sesquiterpene alcohol obtained by the direct distillation of essential oil from plants such as Vanillosmopsis erythropappa and Matricaria chamomilla. (-)-α-Bisabolol has generated considerable economic interest, as it possesses a delicate floral odour and has been shown to have antiseptic and gastroprotective activities. In this study, (-)-α-bisabolol was tested in standardised rodent models by gavage administration at doses of 100 and 200 mg/kg in the models of inflammation and 25 and 50 mg/kg in the models of nociception. In the inflammatory models of paw oedema induced by carrageenan and dextran, the mice treated with (-)-α-bisabolol showed smaller oedemas compared to animals treated only with the vehicle. (-)-α-Bisabolol was capable of reducing paw oedemas induced by 5-HT but not oedemas induced by histamine. (-)-α-Bisabolol demonstrated anti-nociceptive activity in the models of visceral nociception induced by acetic acid and in the second phase of the nociception test induced by the intraplantar administration of formalin. (-)-α-Bisabolol did not have any effect in a thermal nociception model using a hot plate but was able to diminish mechanical inflammatory hypernociception evoked by carrageenan. These findings suggest that the anti-nociceptive action of (-)-α-bisabolol is not linked to a central mechanism but instead is related to the inflammatory process. (-)-α-Bisabolol was able to decrease leukocyte migration, protein extravasations and the amount of TNF-α to the peritoneal cavity in response to carrageenan. Additionally, (-)-α-bisabolol reduced neutrophil degranulation in response to phorbol-myristate-acetate. We demonstrate, for the first time, the peripheral anti-inflammatory and anti-nociceptive activities of (-)-α-bisabolol.

Antinociceptive activity of carvacrol (5-isopropyl-2-methylphenol) in mice

“…The abdominal constriction response induced by acetic acid is a sensitive procedure to evaluate peripheral and central acting analgesics. It has previously been reported that reduction in the amount of constriction induced by acetic acid may be associated with several drugs by acting through different mechanisms, then being considered a nonselective antinociceptive test [21] . In general, acetic acid causes pain through the release of endogenous substances such as serotonin, histamine, prostaglandins (PGs), bradykinins and substance P [2,19] .…”

Synergistic Interaction of Matricaria Chamomilla Extract with Diclofenac and Indomethacin on Carrageenan‐Induced Paw Inflammation in Rats

“…In the same study, three terpenoids were the main constituents identified using 1 H‐nuclear magnetic resonance: α‐bisabolol, bisabolol oxide A, and guaiazulene [Ortiz et al, ]. It has been suggested that cyclooxygenase (COX) inhibition for chamomile or its constituents is a mechanism of action for their analgesic or anti‐inflammatory properties [Srivastava et al, ; Rocha et al, ; Ortiz et al, ]. COX inhibition by NSAIDs, such as diclofenac and indomethacin, induces analgesic, antipyretic, anti‐inflammatory, and gastropathic effects [Ortiz et al, ; Sostres et al, ].…”

α-bisabolol-loaded lipid-core nanocapsules reduce lipopolysaccharide-induced pulmonary inflammation in mice

“…In the same study, the molecule was able to significantly decrease the MPO activity in the peritoneal fluid of rats with induced peritonitis as well as in an in vitro assay of neutrophil degranulation by using phorbol-myristate-acetate. 55 MIP-2, also known as CXCL2, and KC were reported as being important chemoattractant factors to draw neutrophils to the site of inflammation. [56][57][58] Regarding the effects of α-bis on the production of proinflammatory cytokines, the free form was able to reduce the MIP-2 level only at the highest tested dose.…”