Anti-nociceptive and anti-inflammatory effects of Croton crassifolius ethanol extract

Zhao, Jie; Fang, Fang; Yu, Linzhong; Wang, Guoquan; Lei, Yang

doi:10.1016/j.jep.2012.04.050

Cited by 32 publications

(25 citation statements)

References 35 publications

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“…This test has often been used to assess central antinociceptive activity because of its several advantages, particularly its sensitivity to strong analgesics and the fact that tissue damage it imposes is limited (Zhao et al . ).…”

Section: Resultsmentioning

confidence: 99%

“…Substance P, glutamate and bradykinin are thought to participate in this phase, which is believed to be non-inflammatory pain. The second phase (15-30 min after infection of formalin) is thought to be a response associated to inflammatory pain, resulting in the release of several inflammatory mediators, including histamine, serotonin and prostaglandins (Silva et al, 2013;Almeida et al, 2013;Zhao et al, 2012;Hunskaar and Hole, 1987).…”

Section: Resultsmentioning

confidence: 99%

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3,6-Dimethoxy-6″,6″-Dimethyl-(7,8,2″,3″)-Chromeneflavone, a Flavonoid Isolated fromLonchocarpus AraripensisBenth. (Fabaceae), Reduces Nociceptive Behaviour in Mice

et al. 2015

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Lonchocarpus araripensis Benth. is largely distributed in the northeast region of Brazil. It is popularly known as 'sucupira'. Recent studies have shown that some species of Lonchocarpus have interesting pharmacological activities. In this study, we evaluated the antinociceptive effect of a flavone isolated from L. araripensis. The chemical examination resulted in the isolation of 3,6-dimethoxy-6″,6″-dimethyl-(7,8,2″,3″)-chromeneflavone (DDF). The structure of the compound was established by spectral analysis. Antinociceptive activity of DDF was evaluated by measuring nociception by acetic acid, formalin and hot plate tests. The rota rod test was used to evaluate motor coordination. The results demonstrated that DDF was able to prevent acetic-acid-writhing-induced nociception (p < 0.001) in mice. Furthermore, DDF produced a significant reduction of the nociceptive behaviour at the early and late phases of paw licking in the formalin test. Also, DDF produced an inhibition of the nociceptive behaviour during a hot-plate test. No alteration in motor coordination was observed. These results confirm the hypothesis that DDF reduces the nociceptive behaviour in mice, probably through central mechanisms, but without compromising the motor coordination of animals.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

3,6-Dimethoxy-6″,6″-Dimethyl-(7,8,2″,3″)-Chromeneflavone, a Flavonoid Isolated fromLonchocarpus AraripensisBenth. (Fabaceae), Reduces Nociceptive Behaviour in Mice

et al. 2015

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“…The intraperitoneal administration of agents that irritate serous membranes provokes a stereotyped behaviour in the mice which is characterized by abdominal contractions, movements of the body as a whole and twisting of the dorso-abdominal muscles [22] and a reduction in motor activity and motor incoordination [23]. It has been suggested that acetic acid injection into peritoneal cavity leads to increased levels of cyclooxygenases (COX) and lipoxygenase [24] and indirectly leads to the release of endogenous nociceptive mediators such as PGE 2 and PGF 2α [5,20,[25][26][27][28][29][30], serotonin [22,25,26,29,30], histamine [22,26,30,31], bradykinin [21,22,24], substance P [24,25], cytokines (TNF-α, IL-1β, IL-8) [21,24,25,29] and lipoxygenase products [20], which eventually excites the primary afferent nociceptors [25] that contribute to the development of inflammatory pain [22]. The data presented in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The second phase, commonly denominated late or inflammatory phase (from 15 a 30 min) [29] is associated with the release of local endogenous mediators (histamine, serotonin, prostaglandins and bradykinin) responsible for sensitization of primary and spinal sensory neurons and subsequent activation of the nociceptor [20,25,34]. It is well established that both phases of the formalin test can be inhibited by centrally acting drug, such as narcotics, whereas peripherally acting drugs, such as acetylsalicylic acid, only inhibit the second phase [21,22,29,30]. As presented in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The carrageenan-induced paw oedema test is a well-researched and highly reproducible model, and is therefore frequently chosen for evaluating the acute anti-inflammatory actions of natural products [15,28,29,37]. In general, the development of oedema in the rat hind paw following injection of carrageenan has been described as a biphasic event [5,28,[38][39][40], in which various mediators operate in sequence to produce this inflammatory response [28].…”

Section: Discussionmentioning

confidence: 99%

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Antinociceptive and Anti-inflammatory Activities of Methanol Extract of Ormosia coccinea (Aubl.) Jacks in vivo

Díaz¹,

Cruz²,

Gupta³

et al. 2017

JPP

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Abstract:The present study was conducted to evaluate the antinociceptive and anti-inflammatory activities of methanol extract of rachis of Ormosia coccinea (Aubl.) Jacks (MEOC) using animal models of nociception and inflammation. The antinociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Oral administration of MEOC (500 mg/kg) produced significant (p < 0.05) antinociceptive effects when tested in mice using acetic acid-induced abdominal writhing test and on the inflammatory phase of the formalin test. It was also demonstrated that MEOC had no significant effect on the response latency time to the heat stimulus in the thermal model of the hot plate test. The anti-inflammatory activity of the extract was assessed using carrageenan, histamine and serotonin induced oedema in rat paw. The oral administration of MEOC showed maximum inhibition (64.29%) at 1 h on carrageenan edema, but it did not modify the edema induced by histamine and serotonin. The present results suggest that MEOC has a peripheral antinociceptive and anti-inflammatory action.

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Characterization of the chemical constituents of Croton crassifolius Geisel extract and plasma pharmacokinetics of 6 terpenoids after oral administration by UPLC‐Q/TOF‐MS

Shen,

Zhong,

Jiang

et al. 2024

Phytochemical Analysis

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IntroductionCroton crassifolius Geisel. (CCG) is a traditional Chinese medicine widely used in South China. It has various pharmacological effects and is often used in treating rheumatoid arthritis and gastric and duodenal ulcers. However, the chemical characteristics and its effective constituents are still scarcely studied.ObjectiveTo determine the phytochemical profile of the CCG extract and to investigate the chemical characteristics of terpenoids extracted from rat plasma following oral administration of CCG extract based on UPLC‐Q/TOF‐MS. Moreover, six terpenoids in CCG were quantified, and in vivo pharmacokinetic behavior after oral CCG extract was further explored.ResultsIn total, 56 terpenoids were tentatively identified in the CCG extract and 16 terpenoids were detected in rat plasma after oral CCG extract. In addition, the contents of six terpenoids in CCG were clarified. The plasma quantification method of six terpenoids was further established, validated, and confirmed to have good sensitivity and specificity. The six analytes exhibited excellent linearity in respective concentration ranges (r ≥ 0.998). The intra‐day and inter‐day precisions relative standard deviation (RSD, %) were less than 11.27%, and the accuracies ranged from −7.06% to 9.91%. Stability, extraction recovery, and matrix effect in plasma were within the required limits (RSD < 15%).ConclusionA total of 56 terpenoids were identified in CCG and 16 prototype components in plasma after oral CCG. The validated quantitative method was successfully applied to the simultaneous determination of six major terpenoids in plasma. The pharmacokinetic parameters are clarified, which can guide the clinical application of CCG.

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Anti-nociceptive and anti-inflammatory effects of Croton crassifolius ethanol extract

Cited by 32 publications

References 35 publications

3,6-Dimethoxy-6″,6″-Dimethyl-(7,8,2″,3″)-Chromeneflavone, a Flavonoid Isolated fromLonchocarpus AraripensisBenth. (Fabaceae), Reduces Nociceptive Behaviour in Mice

3,6-Dimethoxy-6″,6″-Dimethyl-(7,8,2″,3″)-Chromeneflavone, a Flavonoid Isolated fromLonchocarpus AraripensisBenth. (Fabaceae), Reduces Nociceptive Behaviour in Mice

Antinociceptive and Anti-inflammatory Activities of Methanol Extract of Ormosia coccinea (Aubl.) Jacks in vivo

Characterization of the chemical constituents of Croton crassifolius Geisel extract and plasma pharmacokinetics of 6 terpenoids after oral administration by UPLC‐Q/TOF‐MS

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