Anti-obese effect of iodine-enriched yolk in cultured adipocytes

Abstract

This study was to investigate the effect of iodine-enriched yolk extract on fat accumulation in vitro in cultured 3T3-L1 adipocyte for confirming the function as the functional food. The extracts of iodineenriched and ordinary yolk at the doses of 5, 10, 50 μg/mL were treated after adipocyte differentiation and their effects in intracellular fat accumulation were compared by the analyzing the oil-red staining, GPDH activity and adipogenic-associated genes. Iodine-enriched yolk with dose of 50 μg/mL significantly inhibited the fat accumulation in adipocytes compared with ordinary yolk. In addition, adipogenic-associated genes, i.e. PPAR-γ2, adipogenin, leptin and adiponectin, was significantly inhibited by treatment of iodine-enriched yolk. However, iodide ion (I-) treatment did not affect the fat accumulation in cultured adipocytes. These results indicate that the iodine bounded components including iodine peptide, not iodide ion, in iodine-enriched yolk may inhibit the fat accumulation in cultured adipocytes.

Selenium and Selenoproteins in Adipose Tissue Physiology and Obesity

“…In view of the key role of iodine in thyroid hormone synthesis and metabolism, the reviewed data may be indicative of the potential involvement of iodine in adipokine regulation and obesity [213]. Particularly, iodine as a constituent of iodine-enriched yolk significantly reduced adipocyte hypertrophy associated with down-regulation of PPARγ, adipogenin, and leptin expressions [214]. Taken together, these findings support the role of Se and iodine interplay in insulin resistance and DM2 both pathogenetically associated with obesity [215].…”